Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10825 and BWI76_RS10830 are separated by 233 nucleotides BWI76_RS10830 and BWI76_RS10835 are separated by 50 nucleotides BWI76_RS10835 and BWI76_RS10840 are separated by 155 nucleotides
BWI76_RS10825: BWI76_RS10825 - deferrochelatase/peroxidase EfeB, at 2,287,428 to 2,288,708
_RS10825
BWI76_RS10830: BWI76_RS10830 - phosphate starvation-inducible protein PhoH, at 2,288,942 to 2,290,006
_RS10830
BWI76_RS10835: BWI76_RS10835 - hypothetical protein, at 2,290,057 to 2,290,350
_RS10835
BWI76_RS10840: BWI76_RS10840 - membrane protein, at 2,290,506 to 2,291,294
_RS10840
Position (kb)
2288
2289
2290
2291 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2288.336 kb on - strand, within BWI76_RS10825 at 2288.580 kb on + strand, within BWI76_RS10825 at 2288.675 kb on + strand at 2288.911 kb on - strand at 2288.981 kb on - strand at 2288.982 kb on + strand at 2289.081 kb on - strand, within BWI76_RS10830 at 2289.090 kb on + strand, within BWI76_RS10830 at 2289.189 kb on + strand, within BWI76_RS10830 at 2289.234 kb on - strand, within BWI76_RS10830 at 2289.537 kb on - strand, within BWI76_RS10830 at 2289.668 kb on + strand, within BWI76_RS10830 at 2289.708 kb on - strand, within BWI76_RS10830 at 2289.997 kb on + strand at 2290.057 kb on + strand at 2290.065 kb on - strand at 2290.082 kb on - strand at 2290.159 kb on + strand, within BWI76_RS10835 at 2290.566 kb on + strand at 2290.618 kb on - strand, within BWI76_RS10840 at 2290.642 kb on + strand, within BWI76_RS10840 at 2290.642 kb on + strand, within BWI76_RS10840 at 2290.652 kb on + strand, within BWI76_RS10840 at 2290.846 kb on + strand, within BWI76_RS10840
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,288,336 - BWI76_RS10825 0.71 -1.4 2,288,580 + BWI76_RS10825 0.90 -0.3 2,288,675 + -0.3 2,288,911 - -0.1 2,288,981 - +0.8 2,288,982 + -4.0 2,289,081 - BWI76_RS10830 0.13 -0.9 2,289,090 + BWI76_RS10830 0.14 -2.2 2,289,189 + BWI76_RS10830 0.23 -1.7 2,289,234 - BWI76_RS10830 0.27 +5.4 2,289,537 - BWI76_RS10830 0.56 -0.6 2,289,668 + BWI76_RS10830 0.68 -0.6 2,289,708 - BWI76_RS10830 0.72 -1.3 2,289,997 + -1.4 2,290,057 + -0.8 2,290,065 - -0.1 2,290,082 - -1.9 2,290,159 + BWI76_RS10835 0.35 -2.2 2,290,566 + -0.5 2,290,618 - BWI76_RS10840 0.14 -0.3 2,290,642 + BWI76_RS10840 0.17 -1.3 2,290,642 + BWI76_RS10840 0.17 +1.3 2,290,652 + BWI76_RS10840 0.19 -2.7 2,290,846 + BWI76_RS10840 0.43 +0.1
Or see this region's nucleotide sequence