Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10705

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10695 and BWI76_RS10700 are separated by 245 nucleotidesBWI76_RS10700 and BWI76_RS10705 are separated by 25 nucleotidesBWI76_RS10705 and BWI76_RS10710 are separated by 212 nucleotides BWI76_RS10695: BWI76_RS10695 - aldehyde dehydrogenase PuuC, at 2,260,130 to 2,261,617 _RS10695 BWI76_RS10700: BWI76_RS10700 - XRE family transcriptional regulator, at 2,261,863 to 2,262,420 _RS10700 BWI76_RS10705: BWI76_RS10705 - Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) (from data), at 2,262,446 to 2,263,210 _RS10705 BWI76_RS10710: BWI76_RS10710 - Glutamate--putrescine ligase (EC 6.3.1.11) (from data), at 2,263,423 to 2,264,841 _RS10710 Position (kb) 2262 2263 2264Strain fitness (log2 ratio) -3 -2 -1 0 1at 2261.470 kb on + strandat 2261.516 kb on + strandat 2261.696 kb on + strandat 2262.144 kb on - strand, within BWI76_RS10700at 2262.196 kb on - strand, within BWI76_RS10700at 2262.321 kb on + strand, within BWI76_RS10700at 2262.329 kb on - strand, within BWI76_RS10700at 2262.473 kb on + strandat 2262.543 kb on + strand, within BWI76_RS10705at 2262.563 kb on + strand, within BWI76_RS10705at 2262.639 kb on - strand, within BWI76_RS10705at 2262.796 kb on - strand, within BWI76_RS10705at 2262.916 kb on + strand, within BWI76_RS10705at 2262.928 kb on + strand, within BWI76_RS10705at 2263.134 kb on + strandat 2263.506 kb on + strandat 2263.506 kb on + strandat 2264.061 kb on + strand, within BWI76_RS10710at 2264.127 kb on + strand, within BWI76_RS10710at 2264.135 kb on + strand, within BWI76_RS10710

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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2,261,470 + +0.7
2,261,516 + +0.4
2,261,696 + -0.1
2,262,144 - BWI76_RS10700 0.50 -2.3
2,262,196 - BWI76_RS10700 0.60 +1.1
2,262,321 + BWI76_RS10700 0.82 +1.2
2,262,329 - BWI76_RS10700 0.84 +0.7
2,262,473 + +1.0
2,262,543 + BWI76_RS10705 0.13 -0.3
2,262,563 + BWI76_RS10705 0.15 -3.3
2,262,639 - BWI76_RS10705 0.25 -3.4
2,262,796 - BWI76_RS10705 0.46 -2.7
2,262,916 + BWI76_RS10705 0.61 -1.0
2,262,928 + BWI76_RS10705 0.63 +0.1
2,263,134 + -1.2
2,263,506 + -0.4
2,263,506 + +1.7
2,264,061 + BWI76_RS10710 0.45 -0.4
2,264,127 + BWI76_RS10710 0.50 -0.1
2,264,135 + BWI76_RS10710 0.50 -1.9

Or see this region's nucleotide sequence