Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10695 and BWI76_RS10700 are separated by 245 nucleotides BWI76_RS10700 and BWI76_RS10705 are separated by 25 nucleotides BWI76_RS10705 and BWI76_RS10710 are separated by 212 nucleotides
BWI76_RS10695: BWI76_RS10695 - aldehyde dehydrogenase PuuC, at 2,260,130 to 2,261,617
_RS10695
BWI76_RS10700: BWI76_RS10700 - XRE family transcriptional regulator, at 2,261,863 to 2,262,420
_RS10700
BWI76_RS10705: BWI76_RS10705 - Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) (from data) , at 2,262,446 to 2,263,210
_RS10705
BWI76_RS10710: BWI76_RS10710 - Glutamate--putrescine ligase (EC 6.3.1.11) (from data) , at 2,263,423 to 2,264,841
_RS10710
Position (kb)
2262
2263
2264 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2261.470 kb on + strand at 2261.516 kb on + strand at 2261.696 kb on + strand at 2262.144 kb on - strand, within BWI76_RS10700 at 2262.196 kb on - strand, within BWI76_RS10700 at 2262.321 kb on + strand, within BWI76_RS10700 at 2262.329 kb on - strand, within BWI76_RS10700 at 2262.473 kb on + strand at 2262.543 kb on + strand, within BWI76_RS10705 at 2262.563 kb on + strand, within BWI76_RS10705 at 2262.639 kb on - strand, within BWI76_RS10705 at 2262.796 kb on - strand, within BWI76_RS10705 at 2262.916 kb on + strand, within BWI76_RS10705 at 2262.928 kb on + strand, within BWI76_RS10705 at 2263.134 kb on + strand at 2263.506 kb on + strand at 2263.506 kb on + strand at 2264.061 kb on + strand, within BWI76_RS10710 at 2264.127 kb on + strand, within BWI76_RS10710 at 2264.135 kb on + strand, within BWI76_RS10710
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 2,261,470 + +0.7 2,261,516 + +0.4 2,261,696 + -0.1 2,262,144 - BWI76_RS10700 0.50 -2.3 2,262,196 - BWI76_RS10700 0.60 +1.1 2,262,321 + BWI76_RS10700 0.82 +1.2 2,262,329 - BWI76_RS10700 0.84 +0.7 2,262,473 + +1.0 2,262,543 + BWI76_RS10705 0.13 -0.3 2,262,563 + BWI76_RS10705 0.15 -3.3 2,262,639 - BWI76_RS10705 0.25 -3.4 2,262,796 - BWI76_RS10705 0.46 -2.7 2,262,916 + BWI76_RS10705 0.61 -1.0 2,262,928 + BWI76_RS10705 0.63 +0.1 2,263,134 + -1.2 2,263,506 + -0.4 2,263,506 + +1.7 2,264,061 + BWI76_RS10710 0.45 -0.4 2,264,127 + BWI76_RS10710 0.50 -0.1 2,264,135 + BWI76_RS10710 0.50 -1.9
Or see this region's nucleotide sequence