Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08100 and BWI76_RS08105 overlap by 1 nucleotides BWI76_RS08105 and BWI76_RS08110 are separated by 163 nucleotides BWI76_RS08110 and BWI76_RS08115 are separated by 356 nucleotides
BWI76_RS08100: BWI76_RS08100 - glutamate/aspartate transporter permease GltK, at 1,717,092 to 1,717,766
_RS08100
BWI76_RS08105: BWI76_RS08105 - glutamate/aspartate ABC transporter permease GltJ, at 1,717,766 to 1,718,506
_RS08105
BWI76_RS08110: BWI76_RS08110 - glutamate/aspartate ABC transporter substrate-binding protein, at 1,718,670 to 1,719,581
_RS08110
BWI76_RS08115: BWI76_RS08115 - apolipoprotein N-acyltransferase, at 1,719,938 to 1,721,476
_RS08115
Position (kb)
1718
1719
1720 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1717.699 kb on + strand at 1717.856 kb on + strand, within BWI76_RS08105 at 1717.883 kb on + strand, within BWI76_RS08105 at 1717.906 kb on - strand, within BWI76_RS08105 at 1718.024 kb on - strand, within BWI76_RS08105 at 1718.034 kb on - strand, within BWI76_RS08105 at 1718.122 kb on + strand, within BWI76_RS08105 at 1718.210 kb on - strand, within BWI76_RS08105 at 1718.244 kb on - strand, within BWI76_RS08105 at 1718.333 kb on + strand, within BWI76_RS08105 at 1718.416 kb on + strand, within BWI76_RS08105 at 1718.703 kb on - strand at 1719.091 kb on - strand, within BWI76_RS08110 at 1719.115 kb on + strand, within BWI76_RS08110 at 1719.115 kb on + strand, within BWI76_RS08110 at 1719.377 kb on + strand, within BWI76_RS08110
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,717,699 + -0.7 1,717,856 + BWI76_RS08105 0.12 -1.4 1,717,883 + BWI76_RS08105 0.16 +1.0 1,717,906 - BWI76_RS08105 0.19 -0.6 1,718,024 - BWI76_RS08105 0.35 -0.1 1,718,034 - BWI76_RS08105 0.36 -0.4 1,718,122 + BWI76_RS08105 0.48 -1.5 1,718,210 - BWI76_RS08105 0.60 +0.7 1,718,244 - BWI76_RS08105 0.65 -0.3 1,718,333 + BWI76_RS08105 0.77 +0.8 1,718,416 + BWI76_RS08105 0.88 -1.6 1,718,703 - +1.8 1,719,091 - BWI76_RS08110 0.46 -0.6 1,719,115 + BWI76_RS08110 0.49 -1.3 1,719,115 + BWI76_RS08110 0.49 -1.8 1,719,377 + BWI76_RS08110 0.78 -2.7
Or see this region's nucleotide sequence