Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06065 and BWI76_RS06070 are separated by 11 nucleotides BWI76_RS06070 and BWI76_RS06075 overlap by 4 nucleotides BWI76_RS06075 and BWI76_RS06080 overlap by 4 nucleotides BWI76_RS06080 and BWI76_RS06085 are separated by 82 nucleotides
BWI76_RS06065: BWI76_RS06065 - taurine ABC transporter substrate-binding protein, at 1,274,898 to 1,275,860
_RS06065
BWI76_RS06070: BWI76_RS06070 - taurine transporter ATP-binding subunit, at 1,275,872 to 1,276,639
_RS06070
BWI76_RS06075: BWI76_RS06075 - taurine transporter subunit, at 1,276,636 to 1,277,463
_RS06075
BWI76_RS06080: BWI76_RS06080 - taurine dioxygenase, at 1,277,460 to 1,278,311
_RS06080
BWI76_RS06085: BWI76_RS06085 - hypothetical protein, at 1,278,394 to 1,280,112
_RS06085
Position (kb)
1276
1277
1278 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1275.767 kb on + strand at 1276.057 kb on - strand, within BWI76_RS06070 at 1276.071 kb on - strand, within BWI76_RS06070 at 1276.071 kb on - strand, within BWI76_RS06070 at 1276.143 kb on - strand, within BWI76_RS06070 at 1276.221 kb on + strand, within BWI76_RS06070 at 1276.606 kb on - strand at 1276.708 kb on + strand at 1276.735 kb on + strand, within BWI76_RS06075 at 1277.315 kb on + strand, within BWI76_RS06075 at 1277.902 kb on - strand, within BWI76_RS06080 at 1278.216 kb on + strand, within BWI76_RS06080 at 1278.224 kb on - strand, within BWI76_RS06080 at 1278.257 kb on + strand at 1278.460 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,275,767 + -0.3 1,276,057 - BWI76_RS06070 0.24 -2.2 1,276,071 - BWI76_RS06070 0.26 +2.2 1,276,071 - BWI76_RS06070 0.26 +0.6 1,276,143 - BWI76_RS06070 0.35 -3.9 1,276,221 + BWI76_RS06070 0.45 +1.2 1,276,606 - +0.9 1,276,708 + -0.1 1,276,735 + BWI76_RS06075 0.12 -0.4 1,277,315 + BWI76_RS06075 0.82 -4.7 1,277,902 - BWI76_RS06080 0.52 +0.0 1,278,216 + BWI76_RS06080 0.89 +0.7 1,278,224 - BWI76_RS06080 0.90 +1.9 1,278,257 + -0.7 1,278,460 + +0.3
Or see this region's nucleotide sequence