Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05775 and BWI76_RS05780 overlap by 4 nucleotides BWI76_RS05780 and BWI76_RS05785 overlap by 4 nucleotides BWI76_RS05785 and BWI76_RS05790 overlap by 4 nucleotides BWI76_RS05790 and BWI76_RS05795 are separated by 551 nucleotides
BWI76_RS05775: BWI76_RS05775 - hypothetical protein, at 1,216,908 to 1,217,228
_RS05775
BWI76_RS05780: BWI76_RS05780 - hypothetical protein, at 1,217,225 to 1,217,452
_RS05780
BWI76_RS05785: BWI76_RS05785 - CI repressor, at 1,217,449 to 1,218,000
_RS05785
BWI76_RS05790: BWI76_RS05790 - phage DNA-binding protein, at 1,217,997 to 1,218,263
_RS05790
BWI76_RS05795: BWI76_RS05795 - hypothetical protein, at 1,218,815 to 1,219,552
_RS05795
Position (kb)
1217
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1 at 1217.187 kb on + strand, within BWI76_RS05775 at 1217.187 kb on + strand, within BWI76_RS05775 at 1217.190 kb on - strand, within BWI76_RS05775 at 1217.692 kb on - strand, within BWI76_RS05785 at 1217.760 kb on - strand, within BWI76_RS05785 at 1217.812 kb on - strand, within BWI76_RS05785 at 1218.149 kb on + strand, within BWI76_RS05790 at 1218.309 kb on + strand at 1218.354 kb on - strand at 1218.422 kb on - strand at 1218.422 kb on - strand at 1218.756 kb on + strand at 1218.859 kb on + strand at 1218.920 kb on - strand, within BWI76_RS05795 at 1218.934 kb on - strand, within BWI76_RS05795 at 1219.129 kb on + strand, within BWI76_RS05795 at 1219.137 kb on - strand at 1219.192 kb on - strand, within BWI76_RS05795 at 1219.233 kb on - strand, within BWI76_RS05795
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,217,187 + BWI76_RS05775 0.87 -1.5 1,217,187 + BWI76_RS05775 0.87 -0.5 1,217,190 - BWI76_RS05775 0.88 -1.7 1,217,692 - BWI76_RS05785 0.44 -0.6 1,217,760 - BWI76_RS05785 0.56 -1.0 1,217,812 - BWI76_RS05785 0.66 -2.1 1,218,149 + BWI76_RS05790 0.57 -2.1 1,218,309 + -0.1 1,218,354 - -1.8 1,218,422 - -1.1 1,218,422 - -0.7 1,218,756 + -0.2 1,218,859 + -1.8 1,218,920 - BWI76_RS05795 0.14 +0.1 1,218,934 - BWI76_RS05795 0.16 +0.1 1,219,129 + BWI76_RS05795 0.43 -0.3 1,219,137 - -0.1 1,219,192 - BWI76_RS05795 0.51 -2.2 1,219,233 - BWI76_RS05795 0.57 +0.2
Or see this region's nucleotide sequence