Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05060 and BWI76_RS05065 overlap by 4 nucleotides BWI76_RS05065 and BWI76_RS05070 overlap by 4 nucleotides BWI76_RS05070 and BWI76_RS05075 overlap by 1 nucleotides BWI76_RS05075 and BWI76_RS05080 are separated by 20 nucleotides
BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271
_RS05060
BWI76_RS05065: BWI76_RS05065 - type II secretion system protein GspI, at 1,061,268 to 1,061,633
_RS05065
BWI76_RS05070: BWI76_RS05070 - type II secretion system protein GspH, at 1,061,630 to 1,062,142
_RS05070
BWI76_RS05075: BWI76_RS05075 - type II secretion system protein GspG, at 1,062,142 to 1,062,564
_RS05075
BWI76_RS05080: BWI76_RS05080 - type II secretion protein F, at 1,062,585 to 1,063,790
_RS05080
Position (kb) 1062
1063 Strain fitness (log2 ratio) -2
-1
0
1 at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.252 kb on - strand at 1061.451 kb on - strand, within BWI76_RS05065 at 1061.780 kb on - strand, within BWI76_RS05070 at 1062.031 kb on - strand, within BWI76_RS05070 at 1062.129 kb on - strand at 1062.201 kb on + strand, within BWI76_RS05075 at 1062.687 kb on - strand at 1062.687 kb on - strand at 1062.687 kb on - strand at 1062.845 kb on + strand, within BWI76_RS05080 at 1062.942 kb on - strand, within BWI76_RS05080 at 1063.081 kb on + strand, within BWI76_RS05080 at 1063.193 kb on + strand, within BWI76_RS05080 at 1063.279 kb on - strand, within BWI76_RS05080 at 1063.339 kb on - strand, within BWI76_RS05080 at 1063.339 kb on - strand, within BWI76_RS05080 at 1063.410 kb on - strand, within BWI76_RS05080
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,061,244 + -2.2 1,061,244 + +0.0 1,061,244 + -0.5 1,061,252 - -0.5 1,061,451 - BWI76_RS05065 0.50 -2.0 1,061,780 - BWI76_RS05070 0.29 -0.8 1,062,031 - BWI76_RS05070 0.78 -1.6 1,062,129 - -0.3 1,062,201 + BWI76_RS05075 0.14 -1.6 1,062,687 - -0.4 1,062,687 - +0.3 1,062,687 - +0.1 1,062,845 + BWI76_RS05080 0.22 +1.3 1,062,942 - BWI76_RS05080 0.30 +0.2 1,063,081 + BWI76_RS05080 0.41 -0.6 1,063,193 + BWI76_RS05080 0.50 +0.7 1,063,279 - BWI76_RS05080 0.58 -0.2 1,063,339 - BWI76_RS05080 0.63 -0.0 1,063,339 - BWI76_RS05080 0.63 +0.3 1,063,410 - BWI76_RS05080 0.68 -0.0
Or see this region's nucleotide sequence