Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05005

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04995 and BWI76_RS05000 are separated by 118 nucleotidesBWI76_RS05000 and BWI76_RS05005 overlap by 4 nucleotidesBWI76_RS05005 and BWI76_RS05010 are separated by 51 nucleotidesBWI76_RS05010 and BWI76_RS05015 are separated by 61 nucleotides BWI76_RS04995: BWI76_RS04995 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,047,849 to 1,048,640 _RS04995 BWI76_RS05000: BWI76_RS05000 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,048,759 to 1,049,238 _RS05000 BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632 _RS05005 BWI76_RS05010: BWI76_RS05010 - tRNA glutamyl-Q synthetase, at 1,050,684 to 1,051,565 _RS05010 BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082 _RS05015 Position (kb) 1049 1050 1051Strain fitness (log2 ratio) -3 -2 -1 0 1at 1048.361 kb on - strand, within BWI76_RS04995at 1048.377 kb on - strand, within BWI76_RS04995at 1048.451 kb on + strand, within BWI76_RS04995at 1048.477 kb on - strand, within BWI76_RS04995at 1048.482 kb on + strand, within BWI76_RS04995at 1049.808 kb on - strand, within BWI76_RS05005at 1050.599 kb on - strandat 1050.692 kb on - strandat 1050.692 kb on - strandat 1050.761 kb on + strandat 1050.844 kb on + strand, within BWI76_RS05010at 1050.844 kb on + strand, within BWI76_RS05010at 1050.852 kb on - strand, within BWI76_RS05010at 1051.312 kb on - strand, within BWI76_RS05010at 1051.541 kb on + strandat 1051.571 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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1,048,361 - BWI76_RS04995 0.65 +0.5
1,048,377 - BWI76_RS04995 0.67 -1.4
1,048,451 + BWI76_RS04995 0.76 -1.6
1,048,477 - BWI76_RS04995 0.79 +0.1
1,048,482 + BWI76_RS04995 0.80 +1.3
1,049,808 - BWI76_RS05005 0.41 -1.0
1,050,599 - +0.8
1,050,692 - -0.5
1,050,692 - -0.5
1,050,761 + -2.8
1,050,844 + BWI76_RS05010 0.18 +0.7
1,050,844 + BWI76_RS05010 0.18 -3.1
1,050,852 - BWI76_RS05010 0.19 +0.3
1,051,312 - BWI76_RS05010 0.71 +1.6
1,051,541 + +0.4
1,051,571 + -0.1

Or see this region's nucleotide sequence