Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04995 and BWI76_RS05000 are separated by 118 nucleotides BWI76_RS05000 and BWI76_RS05005 overlap by 4 nucleotides BWI76_RS05005 and BWI76_RS05010 are separated by 51 nucleotides BWI76_RS05010 and BWI76_RS05015 are separated by 61 nucleotides
BWI76_RS04995: BWI76_RS04995 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,047,849 to 1,048,640
_RS04995
BWI76_RS05000: BWI76_RS05000 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,048,759 to 1,049,238
_RS05000
BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632
_RS05005
BWI76_RS05010: BWI76_RS05010 - tRNA glutamyl-Q synthetase, at 1,050,684 to 1,051,565
_RS05010
BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082
_RS05015
Position (kb)
1049
1050
1051 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1048.361 kb on - strand, within BWI76_RS04995 at 1048.377 kb on - strand, within BWI76_RS04995 at 1048.451 kb on + strand, within BWI76_RS04995 at 1048.477 kb on - strand, within BWI76_RS04995 at 1048.482 kb on + strand, within BWI76_RS04995 at 1049.808 kb on - strand, within BWI76_RS05005 at 1050.599 kb on - strand at 1050.692 kb on - strand at 1050.692 kb on - strand at 1050.761 kb on + strand at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.852 kb on - strand, within BWI76_RS05010 at 1051.312 kb on - strand, within BWI76_RS05010 at 1051.541 kb on + strand at 1051.571 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 1,048,361 - BWI76_RS04995 0.65 +0.5 1,048,377 - BWI76_RS04995 0.67 -1.4 1,048,451 + BWI76_RS04995 0.76 -1.6 1,048,477 - BWI76_RS04995 0.79 +0.1 1,048,482 + BWI76_RS04995 0.80 +1.3 1,049,808 - BWI76_RS05005 0.41 -1.0 1,050,599 - +0.8 1,050,692 - -0.5 1,050,692 - -0.5 1,050,761 + -2.8 1,050,844 + BWI76_RS05010 0.18 +0.7 1,050,844 + BWI76_RS05010 0.18 -3.1 1,050,852 - BWI76_RS05010 0.19 +0.3 1,051,312 - BWI76_RS05010 0.71 +1.6 1,051,541 + +0.4 1,051,571 + -0.1
Or see this region's nucleotide sequence