Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04005 and BWI76_RS04010 are separated by 108 nucleotides BWI76_RS04010 and BWI76_RS04015 overlap by 10 nucleotides BWI76_RS04015 and BWI76_RS04020 are separated by 284 nucleotides
BWI76_RS04005: BWI76_RS04005 - primosomal protein DnaI, at 829,219 to 829,758
_RS04005
BWI76_RS04010: BWI76_RS04010 - hypothetical protein, at 829,867 to 830,340
_RS04010
BWI76_RS04015: BWI76_RS04015 - membrane protein, at 830,331 to 831,107
_RS04015
BWI76_RS04020: BWI76_RS04020 - 4-hydroxybenzoate 3-monooxygenase, at 831,392 to 832,576
_RS04020
Position (kb) 830
831
832 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 829.789 kb on - strand at 829.807 kb on - strand at 829.888 kb on - strand at 829.991 kb on - strand, within BWI76_RS04010 at 830.052 kb on + strand, within BWI76_RS04010 at 830.106 kb on - strand, within BWI76_RS04010 at 830.152 kb on - strand, within BWI76_RS04010 at 830.228 kb on + strand, within BWI76_RS04010 at 830.236 kb on - strand, within BWI76_RS04010 at 830.297 kb on + strand at 830.297 kb on + strand at 830.305 kb on - strand at 830.305 kb on - strand at 830.383 kb on - strand at 830.566 kb on - strand, within BWI76_RS04015 at 830.566 kb on - strand, within BWI76_RS04015 at 830.625 kb on + strand, within BWI76_RS04015 at 830.763 kb on + strand, within BWI76_RS04015 at 830.778 kb on - strand, within BWI76_RS04015 at 830.926 kb on - strand, within BWI76_RS04015 at 830.934 kb on - strand, within BWI76_RS04015 at 830.950 kb on - strand, within BWI76_RS04015 at 830.973 kb on - strand, within BWI76_RS04015 at 831.008 kb on + strand, within BWI76_RS04015 at 831.063 kb on - strand at 831.117 kb on - strand at 831.197 kb on - strand at 831.382 kb on + strand at 831.474 kb on + strand at 831.488 kb on - strand at 831.509 kb on - strand at 831.509 kb on - strand at 831.546 kb on + strand, within BWI76_RS04020 at 831.625 kb on + strand, within BWI76_RS04020 at 831.659 kb on + strand, within BWI76_RS04020 at 831.996 kb on - strand, within BWI76_RS04020 at 832.095 kb on - strand, within BWI76_RS04020
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 829,789 - +0.3 829,807 - -0.2 829,888 - -1.8 829,991 - BWI76_RS04010 0.26 -0.6 830,052 + BWI76_RS04010 0.39 +0.6 830,106 - BWI76_RS04010 0.50 -0.3 830,152 - BWI76_RS04010 0.60 -0.8 830,228 + BWI76_RS04010 0.76 -2.3 830,236 - BWI76_RS04010 0.78 -0.4 830,297 + -1.4 830,297 + -0.9 830,305 - -2.2 830,305 - -2.1 830,383 - -2.3 830,566 - BWI76_RS04015 0.30 +0.3 830,566 - BWI76_RS04015 0.30 +0.8 830,625 + BWI76_RS04015 0.38 +3.1 830,763 + BWI76_RS04015 0.56 +1.1 830,778 - BWI76_RS04015 0.58 -0.4 830,926 - BWI76_RS04015 0.77 +1.2 830,934 - BWI76_RS04015 0.78 +2.1 830,950 - BWI76_RS04015 0.80 -0.4 830,973 - BWI76_RS04015 0.83 +0.7 831,008 + BWI76_RS04015 0.87 +1.2 831,063 - +0.6 831,117 - +0.3 831,197 - +0.8 831,382 + -1.5 831,474 + -0.2 831,488 - +0.5 831,509 - -1.4 831,509 - +0.3 831,546 + BWI76_RS04020 0.13 -1.5 831,625 + BWI76_RS04020 0.20 -3.2 831,659 + BWI76_RS04020 0.23 -0.4 831,996 - BWI76_RS04020 0.51 -1.9 832,095 - BWI76_RS04020 0.59 -0.9
Or see this region's nucleotide sequence