Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03820 and BWI76_RS03825 are separated by 295 nucleotides BWI76_RS03825 and BWI76_RS03830 are separated by 19 nucleotides BWI76_RS03830 and BWI76_RS03835 are separated by 378 nucleotides
BWI76_RS03820: BWI76_RS03820 - carbon starvation protein A, at 788,913 to 791,063
_RS03820
BWI76_RS03825: BWI76_RS03825 - 4-hydroxyphenylacetate 3-monooxygenase, at 791,359 to 791,871
_RS03825
BWI76_RS03830: BWI76_RS03830 - 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, at 791,891 to 793,453
_RS03830
BWI76_RS03835: BWI76_RS03835 - 4-hydroxyphenylacetate catabolism regulatory protein HpaA, at 793,832 to 794,722
_RS03835
Position (kb) 791
792
793
794 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 790.962 kb on + strand at 790.972 kb on + strand at 791.031 kb on + strand at 791.039 kb on - strand at 791.129 kb on - strand at 791.234 kb on + strand at 791.442 kb on - strand, within BWI76_RS03825 at 791.475 kb on - strand, within BWI76_RS03825 at 791.493 kb on - strand, within BWI76_RS03825 at 791.504 kb on - strand at 791.530 kb on + strand, within BWI76_RS03825 at 791.652 kb on - strand, within BWI76_RS03825 at 791.652 kb on - strand, within BWI76_RS03825 at 791.835 kb on - strand at 791.854 kb on + strand at 792.365 kb on - strand, within BWI76_RS03830 at 792.539 kb on + strand, within BWI76_RS03830 at 792.703 kb on - strand, within BWI76_RS03830 at 792.727 kb on + strand, within BWI76_RS03830 at 792.738 kb on - strand, within BWI76_RS03830 at 792.803 kb on - strand, within BWI76_RS03830 at 792.818 kb on - strand, within BWI76_RS03830 at 792.851 kb on + strand, within BWI76_RS03830 at 792.954 kb on + strand at 793.098 kb on + strand, within BWI76_RS03830 at 793.218 kb on - strand, within BWI76_RS03830 at 793.264 kb on + strand, within BWI76_RS03830 at 793.269 kb on + strand, within BWI76_RS03830 at 793.272 kb on - strand, within BWI76_RS03830 at 793.350 kb on - strand at 793.381 kb on + strand at 793.389 kb on - strand at 793.400 kb on + strand at 793.421 kb on + strand at 793.571 kb on - strand at 793.590 kb on - strand at 793.628 kb on + strand at 794.012 kb on + strand, within BWI76_RS03835 at 794.108 kb on - strand, within BWI76_RS03835 at 794.159 kb on + strand, within BWI76_RS03835 at 794.411 kb on + strand, within BWI76_RS03835
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 790,962 + +0.3 790,972 + +0.9 791,031 + -0.1 791,039 - +0.3 791,129 - +0.4 791,234 + -0.7 791,442 - BWI76_RS03825 0.16 +1.3 791,475 - BWI76_RS03825 0.23 -0.4 791,493 - BWI76_RS03825 0.26 +1.5 791,504 - +0.7 791,530 + BWI76_RS03825 0.33 +3.2 791,652 - BWI76_RS03825 0.57 +1.3 791,652 - BWI76_RS03825 0.57 -0.2 791,835 - -0.2 791,854 + -2.6 792,365 - BWI76_RS03830 0.30 +0.1 792,539 + BWI76_RS03830 0.41 -1.3 792,703 - BWI76_RS03830 0.52 +0.8 792,727 + BWI76_RS03830 0.53 -2.9 792,738 - BWI76_RS03830 0.54 -0.0 792,803 - BWI76_RS03830 0.58 -1.4 792,818 - BWI76_RS03830 0.59 +0.8 792,851 + BWI76_RS03830 0.61 +0.2 792,954 + -1.3 793,098 + BWI76_RS03830 0.77 -0.7 793,218 - BWI76_RS03830 0.85 +0.9 793,264 + BWI76_RS03830 0.88 -2.0 793,269 + BWI76_RS03830 0.88 +0.1 793,272 - BWI76_RS03830 0.88 +1.0 793,350 - -3.3 793,381 + +0.7 793,389 - -0.3 793,400 + +0.6 793,421 + +0.5 793,571 - +0.2 793,590 - +0.6 793,628 + +1.5 794,012 + BWI76_RS03835 0.20 +0.6 794,108 - BWI76_RS03835 0.31 +0.7 794,159 + BWI76_RS03835 0.37 -0.8 794,411 + BWI76_RS03835 0.65 +1.6
Or see this region's nucleotide sequence