Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01775

Experiment: K. M5a1 KLEB23 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01770 and BWI76_RS01775 are separated by 105 nucleotidesBWI76_RS01775 and BWI76_RS01780 are separated by 138 nucleotides BWI76_RS01770: BWI76_RS01770 - transcriptional regulatory protein RtcR, at 361,143 to 362,723 _RS01770 BWI76_RS01775: BWI76_RS01775 - hypothetical protein, at 362,829 to 363,032 _RS01775 BWI76_RS01780: BWI76_RS01780 - SPFH domain / Band 7 family protein, at 363,171 to 364,298 _RS01780 Position (kb) 362 363 364Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 361.874 kb on - strand, within BWI76_RS01770at 361.929 kb on - strand, within BWI76_RS01770at 361.965 kb on - strand, within BWI76_RS01770at 362.224 kb on - strand, within BWI76_RS01770at 362.307 kb on - strand, within BWI76_RS01770at 362.307 kb on - strand, within BWI76_RS01770at 362.374 kb on - strand, within BWI76_RS01770at 362.439 kb on + strandat 362.460 kb on - strand, within BWI76_RS01770at 362.460 kb on - strand, within BWI76_RS01770at 362.460 kb on - strand, within BWI76_RS01770at 362.584 kb on + strandat 362.632 kb on - strandat 362.689 kb on - strandat 362.916 kb on - strand, within BWI76_RS01775at 363.055 kb on - strandat 363.055 kb on - strandat 363.055 kb on - strandat 363.066 kb on + strandat 363.066 kb on + strandat 363.066 kb on + strandat 363.066 kb on + strandat 363.066 kb on + strandat 363.074 kb on - strandat 363.074 kb on - strandat 363.074 kb on - strandat 363.074 kb on - strandat 363.240 kb on - strandat 363.272 kb on + strandat 363.374 kb on + strand, within BWI76_RS01780at 363.377 kb on - strand, within BWI76_RS01780at 363.419 kb on - strand, within BWI76_RS01780at 363.521 kb on + strand, within BWI76_RS01780at 363.617 kb on + strand, within BWI76_RS01780at 363.635 kb on + strand, within BWI76_RS01780at 363.974 kb on + strand, within BWI76_RS01780at 364.030 kb on + strand, within BWI76_RS01780

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10
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361,874 - BWI76_RS01770 0.46 +0.3
361,929 - BWI76_RS01770 0.50 -2.2
361,965 - BWI76_RS01770 0.52 -0.6
362,224 - BWI76_RS01770 0.68 +0.3
362,307 - BWI76_RS01770 0.74 -1.0
362,307 - BWI76_RS01770 0.74 -3.6
362,374 - BWI76_RS01770 0.78 +1.0
362,439 + +0.3
362,460 - BWI76_RS01770 0.83 +2.0
362,460 - BWI76_RS01770 0.83 +0.9
362,460 - BWI76_RS01770 0.83 -0.5
362,584 + +1.1
362,632 - +0.5
362,689 - -0.5
362,916 - BWI76_RS01775 0.43 -2.2
363,055 - -0.4
363,055 - -1.4
363,055 - +0.1
363,066 + +0.5
363,066 + +3.8
363,066 + -1.2
363,066 + -4.4
363,066 + +0.1
363,074 - +0.3
363,074 - -0.9
363,074 - -3.0
363,074 - -0.4
363,240 - -0.1
363,272 + -0.4
363,374 + BWI76_RS01780 0.18 -4.3
363,377 - BWI76_RS01780 0.18 -2.2
363,419 - BWI76_RS01780 0.22 -0.8
363,521 + BWI76_RS01780 0.31 +0.0
363,617 + BWI76_RS01780 0.40 +0.1
363,635 + BWI76_RS01780 0.41 -0.2
363,974 + BWI76_RS01780 0.71 -1.8
364,030 + BWI76_RS01780 0.76 -2.4

Or see this region's nucleotide sequence