Experiment: K. M5a1 KLEB23 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01770 and BWI76_RS01775 are separated by 105 nucleotides BWI76_RS01775 and BWI76_RS01780 are separated by 138 nucleotides
BWI76_RS01770: BWI76_RS01770 - transcriptional regulatory protein RtcR, at 361,143 to 362,723
_RS01770
BWI76_RS01775: BWI76_RS01775 - hypothetical protein, at 362,829 to 363,032
_RS01775
BWI76_RS01780: BWI76_RS01780 - SPFH domain / Band 7 family protein, at 363,171 to 364,298
_RS01780
Position (kb) 362
363
364 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 361.874 kb on - strand, within BWI76_RS01770 at 361.929 kb on - strand, within BWI76_RS01770 at 361.965 kb on - strand, within BWI76_RS01770 at 362.224 kb on - strand, within BWI76_RS01770 at 362.307 kb on - strand, within BWI76_RS01770 at 362.307 kb on - strand, within BWI76_RS01770 at 362.374 kb on - strand, within BWI76_RS01770 at 362.439 kb on + strand at 362.460 kb on - strand, within BWI76_RS01770 at 362.460 kb on - strand, within BWI76_RS01770 at 362.460 kb on - strand, within BWI76_RS01770 at 362.584 kb on + strand at 362.632 kb on - strand at 362.689 kb on - strand at 362.916 kb on - strand, within BWI76_RS01775 at 363.055 kb on - strand at 363.055 kb on - strand at 363.055 kb on - strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.066 kb on + strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.074 kb on - strand at 363.240 kb on - strand at 363.272 kb on + strand at 363.374 kb on + strand, within BWI76_RS01780 at 363.377 kb on - strand, within BWI76_RS01780 at 363.419 kb on - strand, within BWI76_RS01780 at 363.521 kb on + strand, within BWI76_RS01780 at 363.617 kb on + strand, within BWI76_RS01780 at 363.635 kb on + strand, within BWI76_RS01780 at 363.974 kb on + strand, within BWI76_RS01780 at 364.030 kb on + strand, within BWI76_RS01780
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 10 remove 361,874 - BWI76_RS01770 0.46 +0.3 361,929 - BWI76_RS01770 0.50 -2.2 361,965 - BWI76_RS01770 0.52 -0.6 362,224 - BWI76_RS01770 0.68 +0.3 362,307 - BWI76_RS01770 0.74 -1.0 362,307 - BWI76_RS01770 0.74 -3.6 362,374 - BWI76_RS01770 0.78 +1.0 362,439 + +0.3 362,460 - BWI76_RS01770 0.83 +2.0 362,460 - BWI76_RS01770 0.83 +0.9 362,460 - BWI76_RS01770 0.83 -0.5 362,584 + +1.1 362,632 - +0.5 362,689 - -0.5 362,916 - BWI76_RS01775 0.43 -2.2 363,055 - -0.4 363,055 - -1.4 363,055 - +0.1 363,066 + +0.5 363,066 + +3.8 363,066 + -1.2 363,066 + -4.4 363,066 + +0.1 363,074 - +0.3 363,074 - -0.9 363,074 - -3.0 363,074 - -0.4 363,240 - -0.1 363,272 + -0.4 363,374 + BWI76_RS01780 0.18 -4.3 363,377 - BWI76_RS01780 0.18 -2.2 363,419 - BWI76_RS01780 0.22 -0.8 363,521 + BWI76_RS01780 0.31 +0.0 363,617 + BWI76_RS01780 0.40 +0.1 363,635 + BWI76_RS01780 0.41 -0.2 363,974 + BWI76_RS01780 0.71 -1.8 364,030 + BWI76_RS01780 0.76 -2.4
Or see this region's nucleotide sequence