Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27300

Experiment: K. M5a1 KLEB26 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27295 and BWI76_RS27300 overlap by 4 nucleotidesBWI76_RS27300 and BWI76_RS27305 are separated by 2 nucleotides BWI76_RS27295: BWI76_RS27295 - putative lipopolysaccharide heptosyltransferase III, at 5,665,162 to 5,666,238 _RS27295 BWI76_RS27300: BWI76_RS27300 - glucuronic acid transferase, at 5,666,235 to 5,667,362 _RS27300 BWI76_RS27305: BWI76_RS27305 - glycosyl transferase, at 5,667,365 to 5,668,459 _RS27305 Position (kb) 5666 5667 5668Strain fitness (log2 ratio) -2 -1 0 1at 5665.589 kb on + strand, within BWI76_RS27295at 5667.523 kb on + strand, within BWI76_RS27305at 5667.642 kb on + strand, within BWI76_RS27305at 5667.840 kb on + strand, within BWI76_RS27305at 5667.848 kb on - strand, within BWI76_RS27305at 5667.963 kb on + strand, within BWI76_RS27305at 5668.026 kb on - strand, within BWI76_RS27305at 5668.074 kb on - strand, within BWI76_RS27305at 5668.110 kb on + strand, within BWI76_RS27305at 5668.111 kb on + strand, within BWI76_RS27305at 5668.135 kb on + strand, within BWI76_RS27305at 5668.143 kb on - strand, within BWI76_RS27305at 5668.176 kb on - strand, within BWI76_RS27305at 5668.176 kb on - strand, within BWI76_RS27305at 5668.325 kb on + strand, within BWI76_RS27305at 5668.345 kb on + strand, within BWI76_RS27305at 5668.362 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1
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5,665,589 + BWI76_RS27295 0.40 +1.2
5,667,523 + BWI76_RS27305 0.14 -0.3
5,667,642 + BWI76_RS27305 0.25 -2.1
5,667,840 + BWI76_RS27305 0.43 -1.2
5,667,848 - BWI76_RS27305 0.44 -1.2
5,667,963 + BWI76_RS27305 0.55 -0.6
5,668,026 - BWI76_RS27305 0.60 +1.1
5,668,074 - BWI76_RS27305 0.65 +0.5
5,668,110 + BWI76_RS27305 0.68 +0.0
5,668,111 + BWI76_RS27305 0.68 -1.9
5,668,135 + BWI76_RS27305 0.70 +1.2
5,668,143 - BWI76_RS27305 0.71 +1.4
5,668,176 - BWI76_RS27305 0.74 +0.3
5,668,176 - BWI76_RS27305 0.74 +0.6
5,668,325 + BWI76_RS27305 0.88 +0.5
5,668,345 + BWI76_RS27305 0.89 +0.2
5,668,362 + +0.2

Or see this region's nucleotide sequence