Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27255 and BWI76_RS27260 are separated by 214 nucleotides BWI76_RS27260 and BWI76_RS27265 are separated by 9 nucleotides
BWI76_RS27255: BWI76_RS27255 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29) (from data) , at 5,656,513 to 5,657,706
_RS27255
BWI76_RS27260: BWI76_RS27260 - ADP-L-glycero-D-mannoheptose-6-epimerase, at 5,657,921 to 5,658,853
_RS27260
BWI76_RS27265: BWI76_RS27265 - ADP-heptose--LPS heptosyltransferase II, at 5,658,863 to 5,659,921
_RS27265
Position (kb)
5657
5658
5659 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5657.029 kb on - strand, within BWI76_RS27255 at 5657.029 kb on - strand, within BWI76_RS27255 at 5657.134 kb on - strand, within BWI76_RS27255 at 5657.137 kb on + strand, within BWI76_RS27255 at 5657.153 kb on + strand, within BWI76_RS27255 at 5657.275 kb on - strand, within BWI76_RS27255 at 5657.277 kb on + strand, within BWI76_RS27255 at 5657.305 kb on + strand, within BWI76_RS27255 at 5657.313 kb on - strand, within BWI76_RS27255 at 5657.313 kb on - strand, within BWI76_RS27255 at 5657.394 kb on - strand, within BWI76_RS27255 at 5657.447 kb on - strand at 5657.529 kb on + strand, within BWI76_RS27255 at 5657.629 kb on - strand at 5657.663 kb on + strand at 5657.663 kb on + strand at 5657.671 kb on - strand at 5657.671 kb on - strand at 5657.673 kb on - strand at 5657.751 kb on + strand at 5657.758 kb on + strand at 5657.759 kb on - strand at 5657.759 kb on - strand at 5657.759 kb on - strand at 5657.759 kb on - strand at 5657.814 kb on + strand at 5657.815 kb on + strand at 5657.815 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 5,657,029 - BWI76_RS27255 0.43 -1.2 5,657,029 - BWI76_RS27255 0.43 -0.3 5,657,134 - BWI76_RS27255 0.52 -0.4 5,657,137 + BWI76_RS27255 0.52 -2.9 5,657,153 + BWI76_RS27255 0.54 +0.4 5,657,275 - BWI76_RS27255 0.64 -1.1 5,657,277 + BWI76_RS27255 0.64 +1.4 5,657,305 + BWI76_RS27255 0.66 -0.4 5,657,313 - BWI76_RS27255 0.67 +0.3 5,657,313 - BWI76_RS27255 0.67 -0.3 5,657,394 - BWI76_RS27255 0.74 -1.3 5,657,447 - +1.7 5,657,529 + BWI76_RS27255 0.85 -0.0 5,657,629 - -2.1 5,657,663 + -1.2 5,657,663 + +0.7 5,657,671 - +0.0 5,657,671 - -0.2 5,657,673 - +0.5 5,657,751 + -1.9 5,657,758 + +0.2 5,657,759 - +0.1 5,657,759 - -0.1 5,657,759 - +1.1 5,657,759 - +1.1 5,657,814 + +0.2 5,657,815 + +1.0 5,657,815 + +1.0
Or see this region's nucleotide sequence