Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26930 and BWI76_RS26935 are separated by 167 nucleotides BWI76_RS26935 and BWI76_RS26940 are separated by 93 nucleotides BWI76_RS26940 and BWI76_RS26945 are separated by 108 nucleotides BWI76_RS26945 and BWI76_RS26950 are separated by 3 nucleotides
BWI76_RS26930: BWI76_RS26930 - biotin sulfoxide reductase, at 5,585,308 to 5,587,638
_RS26930
BWI76_RS26935: BWI76_RS26935 - OmpA family lipoprotein, at 5,587,806 to 5,588,468
_RS26935
BWI76_RS26940: BWI76_RS26940 - transcriptional regulator, at 5,588,562 to 5,589,578
_RS26940
BWI76_RS26945: BWI76_RS26945 - hypothetical protein, at 5,589,687 to 5,590,436
_RS26945
BWI76_RS26950: BWI76_RS26950 - 2-dehydro-3-deoxygluconokinase, at 5,590,440 to 5,591,378
_RS26950
Position (kb) 5588
5589
5590 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5587.643 kb on + strand at 5587.669 kb on + strand at 5587.877 kb on + strand, within BWI76_RS26935 at 5587.877 kb on + strand, within BWI76_RS26935 at 5587.877 kb on + strand, within BWI76_RS26935 at 5587.885 kb on - strand, within BWI76_RS26935 at 5587.885 kb on - strand, within BWI76_RS26935 at 5587.947 kb on + strand, within BWI76_RS26935 at 5587.948 kb on + strand, within BWI76_RS26935 at 5587.956 kb on - strand, within BWI76_RS26935 at 5587.956 kb on - strand, within BWI76_RS26935 at 5588.049 kb on - strand, within BWI76_RS26935 at 5588.067 kb on - strand, within BWI76_RS26935 at 5588.196 kb on - strand, within BWI76_RS26935 at 5588.280 kb on + strand, within BWI76_RS26935 at 5588.419 kb on + strand at 5588.424 kb on + strand at 5588.503 kb on + strand at 5588.564 kb on + strand at 5588.737 kb on + strand, within BWI76_RS26940 at 5588.845 kb on + strand, within BWI76_RS26940 at 5588.882 kb on + strand, within BWI76_RS26940 at 5588.897 kb on - strand, within BWI76_RS26940 at 5588.925 kb on - strand, within BWI76_RS26940 at 5589.053 kb on + strand, within BWI76_RS26940 at 5589.152 kb on - strand, within BWI76_RS26940 at 5589.172 kb on + strand, within BWI76_RS26940 at 5589.176 kb on + strand, within BWI76_RS26940 at 5589.186 kb on + strand, within BWI76_RS26940 at 5589.194 kb on - strand, within BWI76_RS26940 at 5589.194 kb on - strand, within BWI76_RS26940 at 5589.236 kb on - strand, within BWI76_RS26940 at 5589.410 kb on - strand, within BWI76_RS26940 at 5589.442 kb on + strand, within BWI76_RS26940 at 5589.450 kb on - strand, within BWI76_RS26940 at 5589.611 kb on + strand at 5589.687 kb on - strand at 5589.757 kb on + strand at 5589.821 kb on - strand, within BWI76_RS26945 at 5589.978 kb on - strand, within BWI76_RS26945 at 5590.209 kb on - strand, within BWI76_RS26945 at 5590.250 kb on + strand, within BWI76_RS26945 at 5590.263 kb on + strand, within BWI76_RS26945 at 5590.403 kb on - strand at 5590.562 kb on - strand, within BWI76_RS26950 at 5590.577 kb on + strand, within BWI76_RS26950
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 5,587,643 + -0.1 5,587,669 + -0.7 5,587,877 + BWI76_RS26935 0.11 +0.5 5,587,877 + BWI76_RS26935 0.11 +0.7 5,587,877 + BWI76_RS26935 0.11 -1.5 5,587,885 - BWI76_RS26935 0.12 +1.2 5,587,885 - BWI76_RS26935 0.12 +0.3 5,587,947 + BWI76_RS26935 0.21 +0.9 5,587,948 + BWI76_RS26935 0.21 +0.8 5,587,956 - BWI76_RS26935 0.23 -2.6 5,587,956 - BWI76_RS26935 0.23 +1.3 5,588,049 - BWI76_RS26935 0.37 +0.7 5,588,067 - BWI76_RS26935 0.39 +0.9 5,588,196 - BWI76_RS26935 0.59 +0.7 5,588,280 + BWI76_RS26935 0.71 -0.8 5,588,419 + -0.1 5,588,424 + +1.5 5,588,503 + +0.1 5,588,564 + +0.6 5,588,737 + BWI76_RS26940 0.17 +1.4 5,588,845 + BWI76_RS26940 0.28 +1.3 5,588,882 + BWI76_RS26940 0.31 +0.6 5,588,897 - BWI76_RS26940 0.33 -0.2 5,588,925 - BWI76_RS26940 0.36 +1.4 5,589,053 + BWI76_RS26940 0.48 +1.9 5,589,152 - BWI76_RS26940 0.58 +4.4 5,589,172 + BWI76_RS26940 0.60 +1.3 5,589,176 + BWI76_RS26940 0.60 -0.1 5,589,186 + BWI76_RS26940 0.61 +0.4 5,589,194 - BWI76_RS26940 0.62 -3.3 5,589,194 - BWI76_RS26940 0.62 +0.4 5,589,236 - BWI76_RS26940 0.66 +1.5 5,589,410 - BWI76_RS26940 0.83 +0.8 5,589,442 + BWI76_RS26940 0.87 +2.1 5,589,450 - BWI76_RS26940 0.87 +1.2 5,589,611 + +1.6 5,589,687 - -0.7 5,589,757 + -1.7 5,589,821 - BWI76_RS26945 0.18 +0.9 5,589,978 - BWI76_RS26945 0.39 -0.6 5,590,209 - BWI76_RS26945 0.70 -0.0 5,590,250 + BWI76_RS26945 0.75 +1.2 5,590,263 + BWI76_RS26945 0.77 +0.9 5,590,403 - -0.8 5,590,562 - BWI76_RS26950 0.13 -3.4 5,590,577 + BWI76_RS26950 0.15 -1.2
Or see this region's nucleotide sequence