Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26275 and BWI76_RS26280 are separated by 121 nucleotides BWI76_RS26280 and BWI76_RS26285 are separated by 23 nucleotides BWI76_RS26285 and BWI76_RS26290 overlap by 4 nucleotides
BWI76_RS26275: BWI76_RS26275 - gamma-glutamyltransferase, at 5,438,258 to 5,440,000
_RS26275
BWI76_RS26280: BWI76_RS26280 - hypothetical protein, at 5,440,122 to 5,440,442
_RS26280
BWI76_RS26285: BWI76_RS26285 - glycerophosphodiester phosphodiesterase, at 5,440,466 to 5,441,206
_RS26285
BWI76_RS26290: BWI76_RS26290 - glycerol-3-phosphate ABC transporter ATP-binding protein, at 5,441,203 to 5,442,273
_RS26290
Position (kb) 5440
5441 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5439.138 kb on - strand, within BWI76_RS26275 at 5439.140 kb on + strand, within BWI76_RS26275 at 5439.140 kb on + strand, within BWI76_RS26275 at 5439.148 kb on - strand, within BWI76_RS26275 at 5439.148 kb on - strand, within BWI76_RS26275 at 5439.212 kb on + strand, within BWI76_RS26275 at 5439.231 kb on + strand, within BWI76_RS26275 at 5439.267 kb on + strand, within BWI76_RS26275 at 5439.417 kb on - strand, within BWI76_RS26275 at 5439.503 kb on + strand, within BWI76_RS26275 at 5439.540 kb on - strand, within BWI76_RS26275 at 5439.572 kb on - strand, within BWI76_RS26275 at 5439.678 kb on + strand, within BWI76_RS26275 at 5439.686 kb on - strand, within BWI76_RS26275 at 5439.742 kb on + strand, within BWI76_RS26275 at 5439.854 kb on + strand at 5439.897 kb on + strand at 5439.897 kb on + strand at 5439.905 kb on - strand at 5439.942 kb on - strand at 5439.963 kb on + strand at 5440.096 kb on - strand at 5440.112 kb on + strand at 5440.115 kb on - strand at 5440.178 kb on + strand, within BWI76_RS26280 at 5440.197 kb on - strand, within BWI76_RS26280 at 5440.218 kb on - strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.293 kb on + strand, within BWI76_RS26280 at 5440.601 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.635 kb on - strand, within BWI76_RS26285 at 5440.698 kb on + strand, within BWI76_RS26285 at 5440.943 kb on - strand, within BWI76_RS26285 at 5441.105 kb on + strand, within BWI76_RS26285 at 5441.113 kb on - strand, within BWI76_RS26285
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 5,439,138 - BWI76_RS26275 0.50 +1.5 5,439,140 + BWI76_RS26275 0.51 -4.2 5,439,140 + BWI76_RS26275 0.51 -0.3 5,439,148 - BWI76_RS26275 0.51 -1.2 5,439,148 - BWI76_RS26275 0.51 -0.1 5,439,212 + BWI76_RS26275 0.55 -2.9 5,439,231 + BWI76_RS26275 0.56 +0.6 5,439,267 + BWI76_RS26275 0.58 -3.8 5,439,417 - BWI76_RS26275 0.66 +0.0 5,439,503 + BWI76_RS26275 0.71 -0.4 5,439,540 - BWI76_RS26275 0.74 -2.1 5,439,572 - BWI76_RS26275 0.75 -1.9 5,439,678 + BWI76_RS26275 0.81 -1.7 5,439,686 - BWI76_RS26275 0.82 -0.5 5,439,742 + BWI76_RS26275 0.85 -1.3 5,439,854 + -0.9 5,439,897 + -0.5 5,439,897 + -3.3 5,439,905 - -4.0 5,439,942 - -1.3 5,439,963 + +1.4 5,440,096 - -1.7 5,440,112 + -0.9 5,440,115 - +0.3 5,440,178 + BWI76_RS26280 0.17 -3.1 5,440,197 - BWI76_RS26280 0.23 -3.5 5,440,218 - BWI76_RS26280 0.30 -1.5 5,440,293 + BWI76_RS26280 0.53 -4.0 5,440,293 + BWI76_RS26280 0.53 -1.0 5,440,601 - BWI76_RS26285 0.18 -1.2 5,440,635 - BWI76_RS26285 0.23 -1.5 5,440,635 - BWI76_RS26285 0.23 +0.8 5,440,698 + BWI76_RS26285 0.31 -2.2 5,440,943 - BWI76_RS26285 0.64 -0.9 5,441,105 + BWI76_RS26285 0.86 -2.1 5,441,113 - BWI76_RS26285 0.87 +2.4
Or see this region's nucleotide sequence