Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26005 and BWI76_RS26010 are separated by 31 nucleotides BWI76_RS26010 and BWI76_RS26015 overlap by 11 nucleotides BWI76_RS26015 and BWI76_RS26020 are separated by 108 nucleotides BWI76_RS26020 and BWI76_RS26025 are separated by 277 nucleotides
BWI76_RS26005: BWI76_RS26005 - glutamine amidotransferase, at 5,375,157 to 5,375,720
_RS26005
BWI76_RS26010: BWI76_RS26010 - cell filamentation protein Fic, at 5,375,752 to 5,376,354
_RS26010
BWI76_RS26015: BWI76_RS26015 - DUF2559 domain-containing protein, at 5,376,344 to 5,376,511
_RS26015
BWI76_RS26020: BWI76_RS26020 - peptidylprolyl isomerase A, at 5,376,620 to 5,377,189
_RS26020
BWI76_RS26025: BWI76_RS26025 - MFS transporter TsgA, at 5,377,467 to 5,378,651
_RS26025
Position (kb) 5376
5377 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5375.367 kb on - strand, within BWI76_RS26005 at 5375.781 kb on - strand at 5375.781 kb on - strand at 5375.850 kb on - strand, within BWI76_RS26010 at 5375.870 kb on - strand, within BWI76_RS26010 at 5375.982 kb on - strand, within BWI76_RS26010 at 5376.018 kb on + strand, within BWI76_RS26010 at 5376.137 kb on - strand, within BWI76_RS26010 at 5376.275 kb on + strand, within BWI76_RS26010 at 5376.299 kb on + strand at 5376.307 kb on - strand at 5376.388 kb on - strand, within BWI76_RS26015 at 5376.602 kb on - strand at 5376.626 kb on - strand at 5376.662 kb on - strand at 5376.679 kb on + strand, within BWI76_RS26020 at 5376.730 kb on - strand, within BWI76_RS26020 at 5376.751 kb on - strand, within BWI76_RS26020 at 5376.825 kb on - strand, within BWI76_RS26020 at 5376.830 kb on + strand, within BWI76_RS26020 at 5376.830 kb on + strand, within BWI76_RS26020 at 5376.830 kb on + strand, within BWI76_RS26020 at 5377.089 kb on - strand, within BWI76_RS26020 at 5377.233 kb on - strand at 5377.282 kb on + strand at 5377.291 kb on - strand at 5377.437 kb on - strand at 5377.448 kb on - strand at 5377.464 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 5,375,367 - BWI76_RS26005 0.37 +0.7 5,375,781 - +1.2 5,375,781 - -0.7 5,375,850 - BWI76_RS26010 0.16 -1.1 5,375,870 - BWI76_RS26010 0.20 +0.9 5,375,982 - BWI76_RS26010 0.38 +0.9 5,376,018 + BWI76_RS26010 0.44 -2.7 5,376,137 - BWI76_RS26010 0.64 -4.1 5,376,275 + BWI76_RS26010 0.87 +1.1 5,376,299 + +0.0 5,376,307 - -1.7 5,376,388 - BWI76_RS26015 0.26 -1.6 5,376,602 - +2.0 5,376,626 - -1.9 5,376,662 - -0.6 5,376,679 + BWI76_RS26020 0.10 +0.9 5,376,730 - BWI76_RS26020 0.19 +1.7 5,376,751 - BWI76_RS26020 0.23 +0.5 5,376,825 - BWI76_RS26020 0.36 -0.5 5,376,830 + BWI76_RS26020 0.37 -0.3 5,376,830 + BWI76_RS26020 0.37 -1.6 5,376,830 + BWI76_RS26020 0.37 -2.6 5,377,089 - BWI76_RS26020 0.82 +0.3 5,377,233 - -1.1 5,377,282 + -1.0 5,377,291 - +0.7 5,377,437 - -2.4 5,377,448 - +0.9 5,377,464 + +0.7
Or see this region's nucleotide sequence