Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25230 and BWI76_RS25235 are separated by 209 nucleotides BWI76_RS25235 and BWI76_RS25240 are separated by 14 nucleotides
BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212
_RS25230
BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399
_RS25235
BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400
_RS25240
Position (kb) 5247
5248
5249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5246.538 kb on - strand, within BWI76_RS25230 at 5246.629 kb on + strand, within BWI76_RS25230 at 5246.740 kb on + strand, within BWI76_RS25230 at 5246.845 kb on - strand, within BWI76_RS25230 at 5246.909 kb on + strand, within BWI76_RS25230 at 5246.947 kb on - strand, within BWI76_RS25230 at 5246.968 kb on + strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5246.976 kb on - strand, within BWI76_RS25230 at 5247.247 kb on - strand at 5247.379 kb on - strand at 5247.432 kb on - strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.553 kb on + strand, within BWI76_RS25235 at 5247.605 kb on - strand, within BWI76_RS25235 at 5247.633 kb on + strand, within BWI76_RS25235 at 5247.699 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand at 5247.998 kb on + strand, within BWI76_RS25235 at 5248.166 kb on + strand, within BWI76_RS25235 at 5248.283 kb on + strand, within BWI76_RS25235 at 5248.369 kb on + strand at 5248.369 kb on + strand at 5248.377 kb on - strand at 5248.409 kb on + strand at 5248.489 kb on + strand at 5248.489 kb on + strand at 5248.694 kb on - strand, within BWI76_RS25240 at 5248.730 kb on - strand, within BWI76_RS25240 at 5248.858 kb on - strand, within BWI76_RS25240 at 5248.882 kb on + strand, within BWI76_RS25240 at 5248.907 kb on - strand, within BWI76_RS25240 at 5248.917 kb on + strand, within BWI76_RS25240 at 5249.215 kb on - strand, within BWI76_RS25240 at 5249.395 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 5,246,538 - BWI76_RS25230 0.17 -2.8 5,246,629 + BWI76_RS25230 0.28 -2.9 5,246,740 + BWI76_RS25230 0.42 +0.7 5,246,845 - BWI76_RS25230 0.55 +0.6 5,246,909 + BWI76_RS25230 0.63 +1.2 5,246,947 - BWI76_RS25230 0.67 +1.1 5,246,968 + BWI76_RS25230 0.70 +1.2 5,246,976 - BWI76_RS25230 0.71 +1.6 5,246,976 - BWI76_RS25230 0.71 +1.0 5,246,976 - BWI76_RS25230 0.71 +0.3 5,247,247 - -0.3 5,247,379 - -0.0 5,247,432 - -0.4 5,247,487 + -3.1 5,247,487 + +1.3 5,247,487 + -0.9 5,247,487 + +0.4 5,247,553 + BWI76_RS25235 0.13 -0.4 5,247,605 - BWI76_RS25235 0.19 +0.8 5,247,633 + BWI76_RS25235 0.22 -1.4 5,247,699 - BWI76_RS25235 0.28 -0.5 5,247,883 - BWI76_RS25235 0.47 -0.2 5,247,883 - +0.3 5,247,998 + BWI76_RS25235 0.59 -0.3 5,248,166 + BWI76_RS25235 0.76 -0.1 5,248,283 + BWI76_RS25235 0.88 +1.9 5,248,369 + +0.4 5,248,369 + -0.1 5,248,377 - +1.9 5,248,409 + -1.0 5,248,489 + +0.1 5,248,489 + -1.2 5,248,694 - BWI76_RS25240 0.28 -0.5 5,248,730 - BWI76_RS25240 0.32 +1.6 5,248,858 - BWI76_RS25240 0.45 +3.3 5,248,882 + BWI76_RS25240 0.47 +1.3 5,248,907 - BWI76_RS25240 0.50 +0.4 5,248,917 + BWI76_RS25240 0.51 +0.8 5,249,215 - BWI76_RS25240 0.81 +2.5 5,249,395 + +0.3
Or see this region's nucleotide sequence