Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24445 and BWI76_RS24450 are separated by 54 nucleotides BWI76_RS24450 and BWI76_RS24455 are separated by 273 nucleotides BWI76_RS24455 and BWI76_RS24460 are separated by 58 nucleotides
BWI76_RS24445: BWI76_RS24445 - hypothetical protein, at 5,097,065 to 5,097,346
_RS24445
BWI76_RS24450: BWI76_RS24450 - glycogen synthase, at 5,097,401 to 5,097,610
_RS24450
BWI76_RS24455: BWI76_RS24455 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 5,097,884 to 5,099,317
_RS24455
BWI76_RS24460: BWI76_RS24460 - bifunctional glutamine synthetase adenylyltransferase/deadenyltransferase, at 5,099,376 to 5,102,210
_RS24460
Position (kb)
5097
5098
5099
5100 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5096.969 kb on + strand at 5097.035 kb on + strand at 5097.035 kb on + strand at 5097.035 kb on + strand at 5097.040 kb on + strand at 5097.043 kb on - strand at 5097.050 kb on + strand at 5097.058 kb on - strand at 5097.418 kb on + strand at 5097.675 kb on + strand at 5099.498 kb on - strand at 5099.550 kb on - strand at 5099.550 kb on - strand at 5099.627 kb on - strand at 5099.872 kb on + strand, within BWI76_RS24460 at 5100.118 kb on + strand, within BWI76_RS24460 at 5100.281 kb on - strand, within BWI76_RS24460
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 5,096,969 + +5.4 5,097,035 + +0.8 5,097,035 + +1.9 5,097,035 + +0.2 5,097,040 + -0.2 5,097,043 - +1.3 5,097,050 + -1.3 5,097,058 - +2.4 5,097,418 + -0.5 5,097,675 + -0.3 5,099,498 - -1.2 5,099,550 - -1.3 5,099,550 - +0.7 5,099,627 - -0.3 5,099,872 + BWI76_RS24460 0.17 -3.0 5,100,118 + BWI76_RS24460 0.26 -3.5 5,100,281 - BWI76_RS24460 0.32 -0.0
Or see this region's nucleotide sequence