Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18830

Experiment: K. M5a1 KLEB26 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18820 and BWI76_RS18825 are separated by 39 nucleotidesBWI76_RS18825 and BWI76_RS18830 are separated by 10 nucleotidesBWI76_RS18830 and BWI76_RS18835 are separated by 322 nucleotides BWI76_RS18820: BWI76_RS18820 - nitrogenase molybdenum-iron protein subunit beta, at 3,903,793 to 3,905,355 _RS18820 BWI76_RS18825: BWI76_RS18825 - protein NifT, at 3,905,395 to 3,905,613 _RS18825 BWI76_RS18830: BWI76_RS18830 - protein NifY, at 3,905,624 to 3,906,286 _RS18830 BWI76_RS18835: BWI76_RS18835 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,906,609 to 3,907,982 _RS18835 Position (kb) 3905 3906 3907Strain fitness (log2 ratio) -3 -2 -1 0 1at 3904.936 kb on + strand, within BWI76_RS18820at 3905.033 kb on - strand, within BWI76_RS18820at 3905.256 kb on - strandat 3905.274 kb on + strandat 3905.675 kb on + strandat 3905.848 kb on - strand, within BWI76_RS18830at 3905.885 kb on + strand, within BWI76_RS18830at 3906.358 kb on + strandat 3906.405 kb on + strandat 3906.470 kb on - strandat 3906.540 kb on + strandat 3906.749 kb on + strand, within BWI76_RS18835at 3906.881 kb on + strand, within BWI76_RS18835at 3906.922 kb on - strand, within BWI76_RS18835at 3907.024 kb on - strand, within BWI76_RS18835at 3907.049 kb on + strand, within BWI76_RS18835

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1
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3,904,936 + BWI76_RS18820 0.73 -0.7
3,905,033 - BWI76_RS18820 0.79 -1.2
3,905,256 - -0.6
3,905,274 + +0.7
3,905,675 + -3.7
3,905,848 - BWI76_RS18830 0.34 -2.1
3,905,885 + BWI76_RS18830 0.39 -1.5
3,906,358 + -3.6
3,906,405 + +0.0
3,906,470 - -2.6
3,906,540 + -1.0
3,906,749 + BWI76_RS18835 0.10 -2.8
3,906,881 + BWI76_RS18835 0.20 -3.2
3,906,922 - BWI76_RS18835 0.23 -2.0
3,907,024 - BWI76_RS18835 0.30 -1.6
3,907,049 + BWI76_RS18835 0.32 +0.0

Or see this region's nucleotide sequence