Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotides BWI76_RS12860 and BWI76_RS12865 overlap by 8 nucleotides BWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotides BWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides
BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189
_RS12855
BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860
_RS12860
BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022
_RS12865
BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861
_RS12870
BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557
_RS12875
Position (kb)
2680
2681
2682
2683 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2680.044 kb on + strand, within BWI76_RS12855 at 2680.303 kb on + strand, within BWI76_RS12860 at 2680.518 kb on - strand, within BWI76_RS12860 at 2681.019 kb on - strand, within BWI76_RS12865 at 2681.110 kb on - strand, within BWI76_RS12865 at 2681.268 kb on + strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.368 kb on - strand, within BWI76_RS12865 at 2681.518 kb on - strand, within BWI76_RS12865 at 2681.906 kb on + strand at 2681.921 kb on - strand at 2682.117 kb on + strand at 2682.117 kb on + strand at 2682.205 kb on - strand, within BWI76_RS12870 at 2682.302 kb on + strand, within BWI76_RS12870 at 2682.418 kb on + strand, within BWI76_RS12870 at 2682.797 kb on + strand at 2682.911 kb on + strand at 2683.019 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 2,680,044 + BWI76_RS12855 0.66 +1.2 2,680,303 + BWI76_RS12860 0.17 +0.3 2,680,518 - BWI76_RS12860 0.49 -0.6 2,681,019 - BWI76_RS12865 0.14 -1.4 2,681,110 - BWI76_RS12865 0.22 -5.3 2,681,268 + BWI76_RS12865 0.35 -3.1 2,681,368 - BWI76_RS12865 0.44 +0.2 2,681,368 - BWI76_RS12865 0.44 -1.8 2,681,518 - BWI76_RS12865 0.57 +0.9 2,681,906 + +2.7 2,681,921 - -3.1 2,682,117 + +0.2 2,682,117 + -1.7 2,682,205 - BWI76_RS12870 0.19 -0.3 2,682,302 + BWI76_RS12870 0.31 +1.2 2,682,418 + BWI76_RS12870 0.46 +1.8 2,682,797 + -0.3 2,682,911 + -0.1 2,683,019 + -0.1
Or see this region's nucleotide sequence