Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12835

Experiment: K. M5a1 KLEB26 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotidesBWI76_RS12830 and BWI76_RS12835 are separated by 23 nucleotidesBWI76_RS12835 and BWI76_RS12840 are separated by 27 nucleotides BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619 _RS12825 BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454 _RS12830 BWI76_RS12835: BWI76_RS12835 - restriction endonuclease subunit S, at 2,674,478 to 2,675,350 _RS12835 BWI76_RS12840: BWI76_RS12840 - amino acid ABC transporter permease/ATP-binding protein, at 2,675,378 to 2,676,898 _RS12840 Position (kb) 2674 2675 2676Strain fitness (log2 ratio) -2 -1 0 1at 2674.325 kb on + strand, within BWI76_RS12830at 2674.345 kb on - strand, within BWI76_RS12830at 2674.382 kb on - strandat 2674.421 kb on + strandat 2674.667 kb on - strand, within BWI76_RS12835at 2674.678 kb on - strand, within BWI76_RS12835at 2674.855 kb on - strand, within BWI76_RS12835at 2674.992 kb on - strand, within BWI76_RS12835at 2675.088 kb on - strand, within BWI76_RS12835at 2675.189 kb on + strand, within BWI76_RS12835at 2675.572 kb on - strand, within BWI76_RS12840at 2675.688 kb on + strand, within BWI76_RS12840at 2675.688 kb on + strand, within BWI76_RS12840at 2675.816 kb on + strand, within BWI76_RS12840at 2675.977 kb on + strand, within BWI76_RS12840at 2675.977 kb on + strand, within BWI76_RS12840at 2676.009 kb on + strand, within BWI76_RS12840at 2676.312 kb on + strand, within BWI76_RS12840at 2676.320 kb on - strand, within BWI76_RS12840

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1
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2,674,325 + BWI76_RS12830 0.84 +1.7
2,674,345 - BWI76_RS12830 0.87 -0.1
2,674,382 - -1.7
2,674,421 + +0.9
2,674,667 - BWI76_RS12835 0.22 -2.7
2,674,678 - BWI76_RS12835 0.23 -1.9
2,674,855 - BWI76_RS12835 0.43 -2.1
2,674,992 - BWI76_RS12835 0.59 -1.2
2,675,088 - BWI76_RS12835 0.70 -0.9
2,675,189 + BWI76_RS12835 0.81 -0.9
2,675,572 - BWI76_RS12840 0.13 -0.2
2,675,688 + BWI76_RS12840 0.20 -0.7
2,675,688 + BWI76_RS12840 0.20 -2.2
2,675,816 + BWI76_RS12840 0.29 -2.3
2,675,977 + BWI76_RS12840 0.39 -0.7
2,675,977 + BWI76_RS12840 0.39 +1.6
2,676,009 + BWI76_RS12840 0.41 +0.1
2,676,312 + BWI76_RS12840 0.61 -0.7
2,676,320 - BWI76_RS12840 0.62 +0.6

Or see this region's nucleotide sequence