Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11325

Experiment: K. M5a1 KLEB26 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11315 and BWI76_RS11320 are separated by 38 nucleotidesBWI76_RS11320 and BWI76_RS11325 are separated by 20 nucleotidesBWI76_RS11325 and BWI76_RS11330 are separated by 236 nucleotides BWI76_RS11315: BWI76_RS11315 - tRNA 2-thiouridine(34) synthase MnmA, at 2,385,288 to 2,386,394 _RS11315 BWI76_RS11320: BWI76_RS11320 - NUDIX hydrolase, at 2,386,433 to 2,386,909 _RS11320 BWI76_RS11325: BWI76_RS11325 - 23S rRNA pseudouridine synthase E, at 2,386,930 to 2,387,580 _RS11325 BWI76_RS11330: BWI76_RS11330 - isocitrate dehydrogenase (NADP(+)), at 2,387,817 to 2,389,067 _RS11330 Position (kb) 2386 2387 2388Strain fitness (log2 ratio) -2 -1 0 1at 2387.180 kb on - strand, within BWI76_RS11325at 2387.561 kb on + strandat 2387.608 kb on - strandat 2387.769 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1
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2,387,180 - BWI76_RS11325 0.38 -2.7
2,387,561 + +0.0
2,387,608 - +1.3
2,387,769 + +0.8

Or see this region's nucleotide sequence