Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07610 and BWI76_RS07615 are separated by 14 nucleotides BWI76_RS07615 and BWI76_RS07620 are separated by 10 nucleotides BWI76_RS07620 and BWI76_RS07625 overlap by 84 nucleotides BWI76_RS07625 and BWI76_RS07630 overlap by 4 nucleotides
BWI76_RS07610: BWI76_RS07610 - choline dehydrogenase, at 1,611,459 to 1,613,123
_RS07610
BWI76_RS07615: BWI76_RS07615 - betaine-aldehyde dehydrogenase, at 1,613,138 to 1,614,610
_RS07615
BWI76_RS07620: BWI76_RS07620 - transcriptional regulator BetI, at 1,614,621 to 1,615,214
_RS07620
BWI76_RS07625: BWI76_RS07625 - hypothetical protein, at 1,615,131 to 1,615,346
_RS07625
BWI76_RS07630: BWI76_RS07630 - high-affinity choline transporter BetT, at 1,615,343 to 1,617,376
_RS07630
Position (kb)
1613
1614
1615 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1612.209 kb on - strand, within BWI76_RS07610 at 1612.221 kb on - strand, within BWI76_RS07610 at 1612.354 kb on - strand, within BWI76_RS07610 at 1612.404 kb on - strand, within BWI76_RS07610 at 1612.576 kb on - strand, within BWI76_RS07610 at 1612.678 kb on - strand, within BWI76_RS07610 at 1612.678 kb on - strand, within BWI76_RS07610 at 1612.968 kb on + strand at 1613.568 kb on + strand, within BWI76_RS07615 at 1613.764 kb on - strand, within BWI76_RS07615 at 1613.769 kb on - strand, within BWI76_RS07615 at 1613.777 kb on - strand, within BWI76_RS07615 at 1614.049 kb on + strand, within BWI76_RS07615 at 1614.090 kb on - strand, within BWI76_RS07615 at 1614.458 kb on + strand, within BWI76_RS07615 at 1614.501 kb on + strand at 1614.501 kb on + strand at 1614.605 kb on + strand at 1614.702 kb on + strand, within BWI76_RS07620 at 1614.818 kb on + strand, within BWI76_RS07620 at 1615.001 kb on - strand, within BWI76_RS07620 at 1615.039 kb on + strand, within BWI76_RS07620 at 1615.467 kb on + strand at 1615.478 kb on + strand at 1615.552 kb on + strand, within BWI76_RS07630 at 1615.590 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 1,612,209 - BWI76_RS07610 0.45 -0.9 1,612,221 - BWI76_RS07610 0.46 -0.1 1,612,354 - BWI76_RS07610 0.54 -0.0 1,612,404 - BWI76_RS07610 0.57 +0.7 1,612,576 - BWI76_RS07610 0.67 -0.1 1,612,678 - BWI76_RS07610 0.73 -0.8 1,612,678 - BWI76_RS07610 0.73 +1.5 1,612,968 + -1.3 1,613,568 + BWI76_RS07615 0.29 -3.5 1,613,764 - BWI76_RS07615 0.42 +0.6 1,613,769 - BWI76_RS07615 0.43 -1.2 1,613,777 - BWI76_RS07615 0.43 -0.7 1,614,049 + BWI76_RS07615 0.62 -0.3 1,614,090 - BWI76_RS07615 0.65 +0.4 1,614,458 + BWI76_RS07615 0.90 +0.3 1,614,501 + +0.3 1,614,501 + -0.3 1,614,605 + +1.1 1,614,702 + BWI76_RS07620 0.14 -1.3 1,614,818 + BWI76_RS07620 0.33 +1.8 1,615,001 - BWI76_RS07620 0.64 -0.5 1,615,039 + BWI76_RS07620 0.70 -1.7 1,615,467 + +2.0 1,615,478 + -3.1 1,615,552 + BWI76_RS07630 0.10 +0.8 1,615,590 + +1.6
Or see this region's nucleotide sequence