Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05795 and BWI76_RS05800 overlap by 4 nucleotides BWI76_RS05800 and BWI76_RS05805 are separated by 17 nucleotides BWI76_RS05805 and BWI76_RS05810 overlap by 37 nucleotides
BWI76_RS05795: BWI76_RS05795 - hypothetical protein, at 1,218,815 to 1,219,552
_RS05795
BWI76_RS05800: BWI76_RS05800 - phage transcriptional activator, Ogr/delta, at 1,219,549 to 1,219,794
_RS05800
BWI76_RS05805: BWI76_RS05805 - phage polarity suppression protein, at 1,219,812 to 1,220,378
_RS05805
BWI76_RS05810: BWI76_RS05810 - hypothetical protein, at 1,220,342 to 1,220,527
_RS05810
Position (kb)
1219
1220 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1218.756 kb on + strand at 1218.859 kb on + strand at 1218.920 kb on - strand, within BWI76_RS05795 at 1218.934 kb on - strand, within BWI76_RS05795 at 1219.129 kb on + strand, within BWI76_RS05795 at 1219.137 kb on - strand at 1219.192 kb on - strand, within BWI76_RS05795 at 1219.233 kb on - strand, within BWI76_RS05795 at 1219.320 kb on - strand, within BWI76_RS05795 at 1219.380 kb on + strand, within BWI76_RS05795 at 1219.532 kb on - strand at 1219.752 kb on + strand, within BWI76_RS05800 at 1219.947 kb on - strand, within BWI76_RS05805 at 1219.965 kb on - strand, within BWI76_RS05805 at 1219.980 kb on - strand, within BWI76_RS05805 at 1219.996 kb on + strand, within BWI76_RS05805 at 1220.092 kb on + strand, within BWI76_RS05805 at 1220.105 kb on - strand, within BWI76_RS05805 at 1220.161 kb on + strand, within BWI76_RS05805 at 1220.166 kb on + strand, within BWI76_RS05805 at 1220.166 kb on + strand, within BWI76_RS05805 at 1220.166 kb on + strand, within BWI76_RS05805 at 1220.213 kb on + strand, within BWI76_RS05805 at 1220.221 kb on - strand, within BWI76_RS05805 at 1220.361 kb on + strand at 1220.432 kb on - strand, within BWI76_RS05810 at 1220.628 kb on + strand at 1220.638 kb on + strand at 1220.638 kb on + strand at 1220.656 kb on + strand at 1220.656 kb on + strand at 1220.664 kb on - strand at 1220.672 kb on + strand at 1220.672 kb on + strand at 1220.672 kb on + strand at 1220.677 kb on + strand at 1220.677 kb on + strand at 1220.734 kb on - strand at 1220.774 kb on + strand at 1220.783 kb on + strand at 1220.791 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 1,218,756 + -4.8 1,218,859 + +1.4 1,218,920 - BWI76_RS05795 0.14 -1.4 1,218,934 - BWI76_RS05795 0.16 -1.8 1,219,129 + BWI76_RS05795 0.43 +0.4 1,219,137 - -0.7 1,219,192 - BWI76_RS05795 0.51 -2.4 1,219,233 - BWI76_RS05795 0.57 -1.5 1,219,320 - BWI76_RS05795 0.68 -2.4 1,219,380 + BWI76_RS05795 0.77 +0.8 1,219,532 - -1.4 1,219,752 + BWI76_RS05800 0.83 -2.6 1,219,947 - BWI76_RS05805 0.24 +0.7 1,219,965 - BWI76_RS05805 0.27 -0.4 1,219,980 - BWI76_RS05805 0.30 +0.0 1,219,996 + BWI76_RS05805 0.32 -3.0 1,220,092 + BWI76_RS05805 0.49 -2.1 1,220,105 - BWI76_RS05805 0.52 -3.9 1,220,161 + BWI76_RS05805 0.62 +0.0 1,220,166 + BWI76_RS05805 0.62 -3.0 1,220,166 + BWI76_RS05805 0.62 +0.5 1,220,166 + BWI76_RS05805 0.62 +0.3 1,220,213 + BWI76_RS05805 0.71 -2.9 1,220,221 - BWI76_RS05805 0.72 -0.1 1,220,361 + -0.7 1,220,432 - BWI76_RS05810 0.48 -3.2 1,220,628 + -1.3 1,220,638 + -2.6 1,220,638 + -0.1 1,220,656 + +0.9 1,220,656 + -1.4 1,220,664 - -2.3 1,220,672 + -0.7 1,220,672 + -3.9 1,220,672 + -0.6 1,220,677 + -2.9 1,220,677 + +0.3 1,220,734 - -1.8 1,220,774 + -1.4 1,220,783 + +0.1 1,220,791 - -3.2
Or see this region's nucleotide sequence