Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05745 and BWI76_RS05750 are separated by 72 nucleotides BWI76_RS05750 and BWI76_RS05755 are separated by 270 nucleotides
BWI76_RS05745: BWI76_RS05745 - hypothetical protein, at 1,208,042 to 1,209,280
_RS05745
BWI76_RS05750: BWI76_RS05750 - thiamine biosynthesis protein ThiJ, at 1,209,353 to 1,210,048
_RS05750
BWI76_RS05755: BWI76_RS05755 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 1,210,319 to 1,211,590
_RS05755
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1209.263 kb on + strand at 1209.461 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.610 kb on + strand, within BWI76_RS05750 at 1209.616 kb on - strand, within BWI76_RS05750 at 1209.639 kb on + strand, within BWI76_RS05750 at 1209.703 kb on + strand, within BWI76_RS05750 at 1209.736 kb on + strand, within BWI76_RS05750 at 1209.817 kb on + strand, within BWI76_RS05750 at 1209.820 kb on - strand, within BWI76_RS05750 at 1209.825 kb on - strand, within BWI76_RS05750 at 1209.870 kb on - strand, within BWI76_RS05750 at 1209.921 kb on - strand, within BWI76_RS05750 at 1209.967 kb on - strand, within BWI76_RS05750 at 1209.994 kb on + strand at 1209.994 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.020 kb on - strand at 1210.105 kb on + strand at 1210.107 kb on + strand at 1210.113 kb on - strand at 1210.116 kb on + strand at 1210.131 kb on + strand at 1210.139 kb on - strand at 1210.175 kb on - strand at 1210.280 kb on - strand at 1210.421 kb on + strand at 1210.428 kb on - strand at 1210.507 kb on - strand, within BWI76_RS05755 at 1210.655 kb on - strand, within BWI76_RS05755 at 1211.043 kb on + strand, within BWI76_RS05755
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 1,209,263 + -0.8 1,209,461 + BWI76_RS05750 0.16 +1.0 1,209,473 + BWI76_RS05750 0.17 +0.3 1,209,473 + BWI76_RS05750 0.17 -1.2 1,209,481 - BWI76_RS05750 0.18 +1.1 1,209,481 - BWI76_RS05750 0.18 -0.1 1,209,481 - BWI76_RS05750 0.18 -2.3 1,209,610 + BWI76_RS05750 0.37 -4.1 1,209,616 - BWI76_RS05750 0.38 -0.3 1,209,639 + BWI76_RS05750 0.41 +0.8 1,209,703 + BWI76_RS05750 0.50 -0.4 1,209,736 + BWI76_RS05750 0.55 +0.2 1,209,817 + BWI76_RS05750 0.67 -1.1 1,209,820 - BWI76_RS05750 0.67 -1.5 1,209,825 - BWI76_RS05750 0.68 +0.4 1,209,870 - BWI76_RS05750 0.74 -1.4 1,209,921 - BWI76_RS05750 0.82 +0.2 1,209,967 - BWI76_RS05750 0.88 +1.5 1,209,994 + -4.7 1,209,994 + -2.9 1,210,012 + -0.7 1,210,012 + -0.5 1,210,012 + -2.5 1,210,020 - -2.8 1,210,105 + +1.9 1,210,107 + +0.1 1,210,113 - -0.7 1,210,116 + -0.6 1,210,131 + +0.2 1,210,139 - -0.6 1,210,175 - -2.0 1,210,280 - -3.1 1,210,421 + +1.6 1,210,428 - -0.9 1,210,507 - BWI76_RS05755 0.15 -0.2 1,210,655 - BWI76_RS05755 0.26 -0.1 1,211,043 + BWI76_RS05755 0.57 +0.3
Or see this region's nucleotide sequence