Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04420 and BWI76_RS04425 are separated by 15 nucleotides BWI76_RS04425 and BWI76_RS04430 are separated by 160 nucleotides BWI76_RS04430 and BWI76_RS04435 are separated by 278 nucleotides
BWI76_RS04420: BWI76_RS04420 - oxaloacetate decarboxylase, at 912,522 to 914,288
_RS04420
BWI76_RS04425: BWI76_RS04425 - oxaloacetate decarboxylase subunit gamma, at 914,304 to 914,555
_RS04425
BWI76_RS04430: BWI76_RS04430 - citrate carrier protein, at 914,716 to 916,056
_RS04430
BWI76_RS04435: BWI76_RS04435 - [citrate (pro-3S)-lyase] ligase, at 916,335 to 917,363
_RS04435
Position (kb) 914
915
916
917 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 913.825 kb on - strand, within BWI76_RS04420 at 913.835 kb on - strand, within BWI76_RS04420 at 913.863 kb on - strand, within BWI76_RS04420 at 914.101 kb on + strand at 914.511 kb on + strand, within BWI76_RS04425 at 914.540 kb on - strand at 914.630 kb on - strand at 914.820 kb on - strand at 914.820 kb on - strand at 914.836 kb on - strand at 914.861 kb on + strand, within BWI76_RS04430 at 914.866 kb on - strand, within BWI76_RS04430 at 914.868 kb on + strand, within BWI76_RS04430 at 915.014 kb on - strand, within BWI76_RS04430 at 915.019 kb on - strand, within BWI76_RS04430 at 915.121 kb on + strand, within BWI76_RS04430 at 915.129 kb on - strand, within BWI76_RS04430 at 915.136 kb on + strand, within BWI76_RS04430 at 915.214 kb on - strand, within BWI76_RS04430 at 915.244 kb on + strand at 915.324 kb on + strand, within BWI76_RS04430 at 915.342 kb on - strand, within BWI76_RS04430 at 915.389 kb on + strand, within BWI76_RS04430 at 915.397 kb on - strand, within BWI76_RS04430 at 915.400 kb on + strand, within BWI76_RS04430 at 915.406 kb on + strand, within BWI76_RS04430 at 915.411 kb on + strand at 915.423 kb on + strand, within BWI76_RS04430 at 915.423 kb on + strand, within BWI76_RS04430 at 915.425 kb on + strand, within BWI76_RS04430 at 915.430 kb on + strand, within BWI76_RS04430 at 915.431 kb on - strand, within BWI76_RS04430 at 915.431 kb on - strand, within BWI76_RS04430 at 915.438 kb on - strand, within BWI76_RS04430 at 915.478 kb on + strand, within BWI76_RS04430 at 915.497 kb on - strand, within BWI76_RS04430 at 915.497 kb on - strand, within BWI76_RS04430 at 915.531 kb on - strand, within BWI76_RS04430 at 915.532 kb on - strand, within BWI76_RS04430 at 915.532 kb on - strand, within BWI76_RS04430 at 915.534 kb on - strand, within BWI76_RS04430 at 915.557 kb on - strand, within BWI76_RS04430 at 915.571 kb on + strand, within BWI76_RS04430 at 915.576 kb on - strand, within BWI76_RS04430 at 915.579 kb on - strand, within BWI76_RS04430 at 915.589 kb on - strand, within BWI76_RS04430 at 915.601 kb on - strand, within BWI76_RS04430 at 915.601 kb on - strand, within BWI76_RS04430 at 915.621 kb on + strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.629 kb on - strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.651 kb on + strand, within BWI76_RS04430 at 915.658 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.659 kb on - strand, within BWI76_RS04430 at 915.680 kb on + strand, within BWI76_RS04430 at 915.698 kb on + strand, within BWI76_RS04430 at 915.741 kb on + strand, within BWI76_RS04430 at 915.741 kb on + strand, within BWI76_RS04430 at 915.756 kb on + strand, within BWI76_RS04430 at 915.799 kb on + strand, within BWI76_RS04430 at 915.818 kb on + strand, within BWI76_RS04430 at 915.892 kb on - strand, within BWI76_RS04430 at 915.895 kb on + strand, within BWI76_RS04430 at 915.915 kb on + strand, within BWI76_RS04430 at 915.923 kb on - strand at 915.954 kb on + strand at 915.954 kb on + strand at 915.957 kb on - strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.961 kb on + strand at 915.962 kb on - strand at 915.969 kb on - strand at 915.969 kb on - strand at 915.969 kb on - strand at 915.970 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.039 kb on - strand at 916.044 kb on - strand at 916.068 kb on - strand at 916.238 kb on + strand at 916.294 kb on + strand at 916.294 kb on + strand at 916.303 kb on - strand at 916.389 kb on + strand at 916.390 kb on + strand at 916.390 kb on - strand at 916.397 kb on + strand at 916.398 kb on - strand at 916.418 kb on + strand at 916.418 kb on + strand at 916.426 kb on - strand at 916.438 kb on + strand, within BWI76_RS04435 at 916.461 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.503 kb on + strand, within BWI76_RS04435 at 916.526 kb on - strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.552 kb on + strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.560 kb on - strand, within BWI76_RS04435 at 916.565 kb on + strand, within BWI76_RS04435 at 916.571 kb on + strand, within BWI76_RS04435 at 916.585 kb on + strand, within BWI76_RS04435 at 916.830 kb on - strand, within BWI76_RS04435
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 913,825 - BWI76_RS04420 0.74 +0.8 913,835 - BWI76_RS04420 0.74 +0.4 913,863 - BWI76_RS04420 0.76 +0.7 914,101 + +1.5 914,511 + BWI76_RS04425 0.82 -0.0 914,540 - -2.5 914,630 - +0.7 914,820 - +1.1 914,820 - -0.0 914,836 - -0.6 914,861 + BWI76_RS04430 0.11 -0.9 914,866 - BWI76_RS04430 0.11 -0.2 914,868 + BWI76_RS04430 0.11 -2.0 915,014 - BWI76_RS04430 0.22 -1.1 915,019 - BWI76_RS04430 0.23 -0.3 915,121 + BWI76_RS04430 0.30 +0.9 915,129 - BWI76_RS04430 0.31 -1.3 915,136 + BWI76_RS04430 0.31 -0.6 915,214 - BWI76_RS04430 0.37 +2.8 915,244 + +0.2 915,324 + BWI76_RS04430 0.45 -1.1 915,342 - BWI76_RS04430 0.47 -4.0 915,389 + BWI76_RS04430 0.50 -0.3 915,397 - BWI76_RS04430 0.51 +0.7 915,400 + BWI76_RS04430 0.51 -2.1 915,406 + BWI76_RS04430 0.51 -0.0 915,411 + -0.5 915,423 + BWI76_RS04430 0.53 -0.3 915,423 + BWI76_RS04430 0.53 +0.1 915,425 + BWI76_RS04430 0.53 -2.7 915,430 + BWI76_RS04430 0.53 -0.3 915,431 - BWI76_RS04430 0.53 -3.1 915,431 - BWI76_RS04430 0.53 +0.2 915,438 - BWI76_RS04430 0.54 -0.9 915,478 + BWI76_RS04430 0.57 -1.8 915,497 - BWI76_RS04430 0.58 -0.6 915,497 - BWI76_RS04430 0.58 -1.3 915,531 - BWI76_RS04430 0.61 -0.3 915,532 - BWI76_RS04430 0.61 +1.4 915,532 - BWI76_RS04430 0.61 -0.8 915,534 - BWI76_RS04430 0.61 -0.1 915,557 - BWI76_RS04430 0.63 +0.1 915,571 + BWI76_RS04430 0.64 -1.3 915,576 - BWI76_RS04430 0.64 +0.5 915,579 - BWI76_RS04430 0.64 -0.7 915,589 - BWI76_RS04430 0.65 +0.2 915,601 - BWI76_RS04430 0.66 -0.1 915,601 - BWI76_RS04430 0.66 -2.6 915,621 + BWI76_RS04430 0.67 -2.3 915,629 - BWI76_RS04430 0.68 +0.2 915,629 - BWI76_RS04430 0.68 -1.0 915,629 - BWI76_RS04430 0.68 +1.2 915,651 + BWI76_RS04430 0.70 -2.5 915,651 + BWI76_RS04430 0.70 -0.5 915,651 + BWI76_RS04430 0.70 -1.1 915,651 + BWI76_RS04430 0.70 -2.2 915,651 + BWI76_RS04430 0.70 -2.0 915,651 + BWI76_RS04430 0.70 +1.0 915,651 + BWI76_RS04430 0.70 +0.2 915,651 + BWI76_RS04430 0.70 +1.4 915,651 + +2.5 915,651 + BWI76_RS04430 0.70 -1.3 915,651 + BWI76_RS04430 0.70 -1.9 915,651 + BWI76_RS04430 0.70 -0.9 915,651 + BWI76_RS04430 0.70 +0.5 915,651 + BWI76_RS04430 0.70 -0.1 915,658 - BWI76_RS04430 0.70 -1.1 915,659 - BWI76_RS04430 0.70 -0.8 915,659 - BWI76_RS04430 0.70 -1.0 915,659 - BWI76_RS04430 0.70 +2.1 915,659 - BWI76_RS04430 0.70 -1.0 915,659 - BWI76_RS04430 0.70 -1.6 915,680 + BWI76_RS04430 0.72 -0.3 915,698 + BWI76_RS04430 0.73 -1.5 915,741 + BWI76_RS04430 0.76 +0.3 915,741 + BWI76_RS04430 0.76 +1.0 915,756 + BWI76_RS04430 0.78 -1.9 915,799 + BWI76_RS04430 0.81 -2.8 915,818 + BWI76_RS04430 0.82 -0.3 915,892 - BWI76_RS04430 0.88 -1.3 915,895 + BWI76_RS04430 0.88 -2.3 915,915 + BWI76_RS04430 0.89 -1.9 915,923 - +0.9 915,954 + -2.1 915,954 + -1.3 915,957 - -0.8 915,961 + +2.2 915,961 + +0.1 915,961 + -1.0 915,961 + -0.7 915,961 + +0.5 915,962 - +0.9 915,969 - +2.5 915,969 - -1.3 915,969 - +1.4 915,970 - +0.9 916,039 - +0.1 916,039 - -1.5 916,039 - +0.8 916,039 - -2.8 916,039 - -2.8 916,039 - +0.2 916,039 - -1.7 916,044 - -4.2 916,068 - -0.3 916,238 + +1.3 916,294 + +0.2 916,294 + -1.4 916,303 - +0.2 916,389 + +0.6 916,390 + -1.5 916,390 - +1.5 916,397 + +1.2 916,398 - -1.4 916,418 + +0.9 916,418 + -1.4 916,426 - -3.4 916,438 + BWI76_RS04435 0.10 -3.6 916,461 + BWI76_RS04435 0.12 -0.6 916,503 + BWI76_RS04435 0.16 -0.6 916,503 + BWI76_RS04435 0.16 +0.5 916,526 - BWI76_RS04435 0.19 -1.4 916,552 + BWI76_RS04435 0.21 +1.1 916,552 + BWI76_RS04435 0.21 -0.7 916,560 - BWI76_RS04435 0.22 +1.7 916,560 - BWI76_RS04435 0.22 +0.4 916,565 + BWI76_RS04435 0.22 +0.9 916,571 + BWI76_RS04435 0.23 +0.2 916,585 + BWI76_RS04435 0.24 -0.1 916,830 - BWI76_RS04435 0.48 -0.5
Or see this region's nucleotide sequence