Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02220 and BWI76_RS02225 overlap by 4 nucleotides BWI76_RS02225 and BWI76_RS02230 are separated by 0 nucleotides BWI76_RS02230 and BWI76_RS02235 are separated by 204 nucleotides BWI76_RS02235 and BWI76_RS02240 are separated by 160 nucleotides
BWI76_RS02220: BWI76_RS02220 - carbon-phosphorus lyase subunit PhnH, at 459,421 to 460,005
_RS02220
BWI76_RS02225: BWI76_RS02225 - phosphonate C-P lyase system protein PhnG, at 460,002 to 460,454
_RS02225
BWI76_RS02230: BWI76_RS02230 - phosphonate metabolism transcriptional regulator PhnF, at 460,455 to 461,180
_RS02230
BWI76_RS02235: BWI76_RS02235 - VOC family protein, at 461,385 to 461,819
_RS02235
BWI76_RS02240: BWI76_RS02240 - alkylphosphonate utilization operon protein PhnA, at 461,980 to 462,315
_RS02240
Position (kb) 460
461
462 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 459.794 kb on + strand, within BWI76_RS02220 at 459.804 kb on + strand, within BWI76_RS02220 at 459.906 kb on + strand, within BWI76_RS02220 at 460.008 kb on - strand at 460.181 kb on - strand, within BWI76_RS02225 at 460.182 kb on - strand, within BWI76_RS02225 at 460.319 kb on + strand, within BWI76_RS02225 at 460.342 kb on + strand, within BWI76_RS02225 at 460.546 kb on - strand, within BWI76_RS02230 at 460.654 kb on - strand, within BWI76_RS02230 at 460.691 kb on + strand, within BWI76_RS02230 at 460.718 kb on + strand, within BWI76_RS02230 at 460.744 kb on + strand, within BWI76_RS02230 at 460.750 kb on - strand, within BWI76_RS02230 at 460.752 kb on - strand, within BWI76_RS02230 at 460.762 kb on - strand, within BWI76_RS02230 at 460.821 kb on + strand, within BWI76_RS02230 at 460.890 kb on - strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.196 kb on + strand at 461.409 kb on - strand at 461.556 kb on + strand, within BWI76_RS02235 at 461.634 kb on + strand, within BWI76_RS02235 at 461.664 kb on - strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.738 kb on + strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.746 kb on - strand, within BWI76_RS02235 at 461.751 kb on - strand, within BWI76_RS02235 at 461.774 kb on + strand, within BWI76_RS02235 at 461.777 kb on - strand at 461.800 kb on + strand at 461.809 kb on + strand at 461.815 kb on + strand at 461.824 kb on - strand at 461.828 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.830 kb on - strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.853 kb on + strand at 461.854 kb on - strand at 461.861 kb on - strand at 461.861 kb on - strand at 461.895 kb on + strand at 461.895 kb on + strand at 461.900 kb on + strand at 461.901 kb on - strand at 461.908 kb on - strand at 461.914 kb on + strand at 461.919 kb on + strand at 461.919 kb on + strand at 461.921 kb on + strand at 461.921 kb on + strand at 461.927 kb on - strand at 462.021 kb on - strand, within BWI76_RS02240 at 462.040 kb on - strand, within BWI76_RS02240 at 462.055 kb on + strand at 462.056 kb on + strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.064 kb on - strand, within BWI76_RS02240 at 462.073 kb on + strand, within BWI76_RS02240 at 462.102 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.112 kb on + strand, within BWI76_RS02240 at 462.113 kb on + strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.120 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.121 kb on - strand, within BWI76_RS02240 at 462.144 kb on + strand, within BWI76_RS02240
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 459,794 + BWI76_RS02220 0.64 -1.6 459,804 + BWI76_RS02220 0.65 -4.4 459,906 + BWI76_RS02220 0.83 +1.2 460,008 - +0.0 460,181 - BWI76_RS02225 0.40 +0.6 460,182 - BWI76_RS02225 0.40 -3.0 460,319 + BWI76_RS02225 0.70 -0.9 460,342 + BWI76_RS02225 0.75 +0.6 460,546 - BWI76_RS02230 0.13 +0.2 460,654 - BWI76_RS02230 0.27 +1.1 460,691 + BWI76_RS02230 0.33 -0.8 460,718 + BWI76_RS02230 0.36 +0.3 460,744 + BWI76_RS02230 0.40 -1.4 460,750 - BWI76_RS02230 0.41 -1.5 460,752 - BWI76_RS02230 0.41 -1.7 460,762 - BWI76_RS02230 0.42 -0.3 460,821 + BWI76_RS02230 0.50 -3.2 460,890 - BWI76_RS02230 0.60 -0.5 461,027 + BWI76_RS02230 0.79 +0.4 461,027 + BWI76_RS02230 0.79 -0.3 461,035 - BWI76_RS02230 0.80 +0.3 461,035 - BWI76_RS02230 0.80 -1.5 461,196 + -0.6 461,409 - -0.2 461,556 + BWI76_RS02235 0.39 -4.8 461,634 + BWI76_RS02235 0.57 -0.3 461,664 - BWI76_RS02235 0.64 +2.2 461,738 + BWI76_RS02235 0.81 +0.6 461,738 + BWI76_RS02235 0.81 -0.9 461,738 + BWI76_RS02235 0.81 -0.5 461,746 - BWI76_RS02235 0.83 -0.0 461,746 - BWI76_RS02235 0.83 -2.1 461,746 - BWI76_RS02235 0.83 +0.8 461,746 - BWI76_RS02235 0.83 -0.3 461,751 - BWI76_RS02235 0.84 -0.8 461,774 + BWI76_RS02235 0.89 -1.2 461,777 - -1.5 461,800 + +1.0 461,809 + +0.4 461,815 + +0.5 461,824 - +1.2 461,828 - -0.4 461,830 - +1.3 461,830 - +0.3 461,830 - -2.0 461,830 - -0.9 461,853 + -0.1 461,853 + +0.2 461,853 + -0.8 461,854 - +0.2 461,861 - +1.3 461,861 - -3.1 461,895 + +1.6 461,895 + +0.1 461,900 + +0.3 461,901 - -2.1 461,908 - +0.0 461,914 + -0.8 461,919 + -0.4 461,919 + +0.6 461,921 + +0.4 461,921 + -2.0 461,927 - -1.1 462,021 - BWI76_RS02240 0.12 +1.0 462,040 - BWI76_RS02240 0.18 +1.4 462,055 + +1.4 462,056 + BWI76_RS02240 0.23 -0.7 462,064 - BWI76_RS02240 0.25 -0.6 462,064 - BWI76_RS02240 0.25 -0.1 462,064 - BWI76_RS02240 0.25 +0.2 462,064 - BWI76_RS02240 0.25 +0.3 462,064 - BWI76_RS02240 0.25 +0.0 462,064 - BWI76_RS02240 0.25 -1.1 462,064 - BWI76_RS02240 0.25 -0.7 462,073 + BWI76_RS02240 0.28 -0.1 462,102 + BWI76_RS02240 0.36 -1.6 462,112 + BWI76_RS02240 0.39 +0.5 462,112 + BWI76_RS02240 0.39 -1.4 462,113 + BWI76_RS02240 0.40 +1.8 462,120 - BWI76_RS02240 0.42 +1.4 462,120 - BWI76_RS02240 0.42 -0.9 462,120 - BWI76_RS02240 0.42 -1.1 462,121 - BWI76_RS02240 0.42 -0.4 462,121 - BWI76_RS02240 0.42 +0.3 462,144 + BWI76_RS02240 0.49 +0.4
Or see this region's nucleotide sequence