Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02005 and BWI76_RS02010 are separated by 36 nucleotides BWI76_RS02010 and BWI76_RS02015 are separated by 151 nucleotides BWI76_RS02015 and BWI76_RS02020 are separated by 146 nucleotides
BWI76_RS02005: BWI76_RS02005 - glutathione S-transferase, at 411,855 to 412,523
_RS02005
BWI76_RS02010: BWI76_RS02010 - hypothetical protein, at 412,560 to 412,760
_RS02010
BWI76_RS02015: BWI76_RS02015 - NCS2 family permease, at 412,912 to 414,261
_RS02015
BWI76_RS02020: BWI76_RS02020 - Na+/H+ antiporter, at 414,408 to 416,054
_RS02020
Position (kb) 412
413
414
415 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 411.943 kb on - strand, within BWI76_RS02005 at 411.951 kb on - strand, within BWI76_RS02005 at 411.953 kb on + strand, within BWI76_RS02005 at 411.959 kb on + strand, within BWI76_RS02005 at 412.056 kb on + strand, within BWI76_RS02005 at 412.078 kb on + strand, within BWI76_RS02005 at 412.085 kb on - strand, within BWI76_RS02005 at 412.145 kb on + strand, within BWI76_RS02005 at 412.150 kb on + strand, within BWI76_RS02005 at 412.213 kb on - strand, within BWI76_RS02005 at 412.253 kb on + strand, within BWI76_RS02005 at 412.261 kb on - strand, within BWI76_RS02005 at 412.285 kb on - strand, within BWI76_RS02005 at 412.322 kb on + strand, within BWI76_RS02005 at 412.461 kb on - strand at 412.463 kb on + strand at 412.863 kb on - strand at 413.015 kb on + strand at 413.016 kb on - strand at 413.130 kb on + strand, within BWI76_RS02015 at 413.138 kb on - strand, within BWI76_RS02015 at 413.390 kb on + strand, within BWI76_RS02015 at 413.769 kb on + strand at 413.813 kb on - strand, within BWI76_RS02015 at 413.874 kb on - strand, within BWI76_RS02015 at 413.935 kb on - strand, within BWI76_RS02015 at 414.012 kb on - strand, within BWI76_RS02015 at 414.098 kb on + strand, within BWI76_RS02015 at 414.143 kb on + strand at 414.151 kb on - strand at 414.191 kb on + strand at 414.199 kb on - strand at 414.201 kb on + strand at 414.209 kb on - strand at 414.209 kb on - strand at 414.335 kb on + strand at 414.347 kb on + strand at 414.439 kb on - strand at 414.439 kb on - strand at 414.507 kb on - strand at 414.525 kb on + strand at 414.528 kb on - strand at 414.530 kb on + strand at 414.533 kb on - strand at 414.565 kb on + strand at 414.602 kb on + strand, within BWI76_RS02020 at 414.727 kb on - strand, within BWI76_RS02020 at 414.798 kb on - strand, within BWI76_RS02020 at 414.808 kb on - strand, within BWI76_RS02020 at 414.808 kb on - strand, within BWI76_RS02020 at 415.019 kb on - strand, within BWI76_RS02020 at 415.074 kb on + strand, within BWI76_RS02020 at 415.099 kb on + strand, within BWI76_RS02020 at 415.099 kb on + strand, within BWI76_RS02020 at 415.107 kb on - strand, within BWI76_RS02020 at 415.166 kb on - strand, within BWI76_RS02020
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 411,943 - BWI76_RS02005 0.13 +0.9 411,951 - BWI76_RS02005 0.14 +0.2 411,953 + BWI76_RS02005 0.15 -1.9 411,959 + BWI76_RS02005 0.16 +0.4 412,056 + BWI76_RS02005 0.30 -3.0 412,078 + BWI76_RS02005 0.33 -1.8 412,085 - BWI76_RS02005 0.34 -0.1 412,145 + BWI76_RS02005 0.43 -2.9 412,150 + BWI76_RS02005 0.44 -2.8 412,213 - BWI76_RS02005 0.54 +1.5 412,253 + BWI76_RS02005 0.59 +0.8 412,261 - BWI76_RS02005 0.61 -2.9 412,285 - BWI76_RS02005 0.64 -0.8 412,322 + BWI76_RS02005 0.70 -1.7 412,461 - -2.2 412,463 + -1.1 412,863 - -0.7 413,015 + -3.8 413,016 - -1.3 413,130 + BWI76_RS02015 0.16 -0.5 413,138 - BWI76_RS02015 0.17 -1.2 413,390 + BWI76_RS02015 0.35 +0.6 413,769 + -0.5 413,813 - BWI76_RS02015 0.67 -0.1 413,874 - BWI76_RS02015 0.71 -0.2 413,935 - BWI76_RS02015 0.76 -0.2 414,012 - BWI76_RS02015 0.81 -2.0 414,098 + BWI76_RS02015 0.88 -1.1 414,143 + -0.3 414,151 - -0.6 414,191 + -1.4 414,199 - -0.7 414,201 + +0.9 414,209 - -0.8 414,209 - -1.0 414,335 + -0.5 414,347 + +0.9 414,439 - +0.1 414,439 - -1.1 414,507 - -1.4 414,525 + +0.2 414,528 - -1.0 414,530 + -0.4 414,533 - -1.2 414,565 + -0.4 414,602 + BWI76_RS02020 0.12 +0.4 414,727 - BWI76_RS02020 0.19 -0.2 414,798 - BWI76_RS02020 0.24 -1.9 414,808 - BWI76_RS02020 0.24 -1.2 414,808 - BWI76_RS02020 0.24 -0.1 415,019 - BWI76_RS02020 0.37 -2.9 415,074 + BWI76_RS02020 0.40 +0.2 415,099 + BWI76_RS02020 0.42 +2.6 415,099 + BWI76_RS02020 0.42 -0.8 415,107 - BWI76_RS02020 0.42 -0.5 415,166 - BWI76_RS02020 0.46 +0.7
Or see this region's nucleotide sequence