Experiment: K. M5a1 KLEB26 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01935 and BWI76_RS01940 are separated by 2 nucleotides BWI76_RS01940 and BWI76_RS01945 are separated by 191 nucleotides BWI76_RS01945 and BWI76_RS01950 are separated by 132 nucleotides BWI76_RS01950 and BWI76_RS01955 are separated by 246 nucleotides BWI76_RS01955 and BWI76_RS01960 are separated by 86 nucleotides
BWI76_RS01935: BWI76_RS01935 - thiamin phosphate synthase, at 394,542 to 394,958
_RS01935
BWI76_RS01940: BWI76_RS01940 - hypothetical protein, at 394,961 to 395,314
_RS01940
BWI76_RS01945: BWI76_RS01945 - hypothetical protein, at 395,506 to 395,753
_RS01945
BWI76_RS01950: BWI76_RS01950 - excinuclease ABC subunit A, at 395,886 to 398,711
_RS01950
BWI76_RS01955: BWI76_RS01955 - ssDNA-binding protein, at 398,958 to 399,485
_RS01955
BWI76_RS01960: BWI76_RS01960 - lytic transglycosylase F, at 399,572 to 401,098
_RS01960
Position (kb) 395
396
397
398
399 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 394.934 kb on - strand at 394.994 kb on + strand at 395.004 kb on + strand, within BWI76_RS01940 at 395.009 kb on + strand, within BWI76_RS01940 at 395.044 kb on + strand, within BWI76_RS01940 at 395.321 kb on + strand at 395.347 kb on - strand at 395.442 kb on + strand at 395.529 kb on - strand at 396.040 kb on - strand at 396.069 kb on - strand at 396.099 kb on - strand at 396.253 kb on - strand, within BWI76_RS01950 at 396.350 kb on - strand, within BWI76_RS01950 at 396.409 kb on + strand, within BWI76_RS01950 at 396.417 kb on - strand, within BWI76_RS01950 at 396.517 kb on - strand, within BWI76_RS01950 at 396.525 kb on + strand, within BWI76_RS01950 at 396.530 kb on + strand, within BWI76_RS01950 at 396.530 kb on + strand, within BWI76_RS01950 at 396.538 kb on - strand, within BWI76_RS01950 at 396.757 kb on + strand, within BWI76_RS01950 at 396.825 kb on - strand, within BWI76_RS01950 at 396.853 kb on - strand, within BWI76_RS01950 at 396.994 kb on + strand, within BWI76_RS01950 at 397.001 kb on + strand, within BWI76_RS01950 at 397.151 kb on + strand, within BWI76_RS01950 at 397.194 kb on + strand, within BWI76_RS01950 at 397.194 kb on + strand, within BWI76_RS01950 at 397.202 kb on - strand, within BWI76_RS01950 at 397.274 kb on + strand, within BWI76_RS01950 at 397.467 kb on + strand, within BWI76_RS01950 at 397.489 kb on - strand, within BWI76_RS01950 at 397.535 kb on - strand, within BWI76_RS01950 at 397.565 kb on + strand, within BWI76_RS01950 at 397.673 kb on - strand, within BWI76_RS01950 at 397.687 kb on + strand, within BWI76_RS01950 at 397.695 kb on - strand, within BWI76_RS01950 at 397.821 kb on + strand at 397.859 kb on - strand, within BWI76_RS01950 at 397.883 kb on - strand, within BWI76_RS01950 at 398.095 kb on - strand, within BWI76_RS01950 at 398.095 kb on - strand, within BWI76_RS01950 at 398.187 kb on + strand, within BWI76_RS01950 at 398.187 kb on + strand, within BWI76_RS01950 at 398.195 kb on - strand, within BWI76_RS01950 at 398.251 kb on - strand, within BWI76_RS01950 at 398.292 kb on - strand, within BWI76_RS01950 at 398.292 kb on - strand, within BWI76_RS01950 at 398.535 kb on + strand at 398.591 kb on - strand at 398.606 kb on - strand at 398.695 kb on + strand at 399.518 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1 remove 394,934 - -1.7 394,994 + -2.1 395,004 + BWI76_RS01940 0.12 -1.7 395,009 + BWI76_RS01940 0.14 -1.2 395,044 + BWI76_RS01940 0.23 -0.9 395,321 + +2.5 395,347 - -3.3 395,442 + -3.9 395,529 - +0.1 396,040 - +0.6 396,069 - -4.3 396,099 - +1.4 396,253 - BWI76_RS01950 0.13 +0.9 396,350 - BWI76_RS01950 0.16 -1.2 396,409 + BWI76_RS01950 0.19 +0.4 396,417 - BWI76_RS01950 0.19 -0.2 396,517 - BWI76_RS01950 0.22 -0.6 396,525 + BWI76_RS01950 0.23 -1.0 396,530 + BWI76_RS01950 0.23 -1.1 396,530 + BWI76_RS01950 0.23 -6.0 396,538 - BWI76_RS01950 0.23 -3.4 396,757 + BWI76_RS01950 0.31 +0.1 396,825 - BWI76_RS01950 0.33 -1.4 396,853 - BWI76_RS01950 0.34 -1.3 396,994 + BWI76_RS01950 0.39 +0.3 397,001 + BWI76_RS01950 0.39 -3.0 397,151 + BWI76_RS01950 0.45 +0.1 397,194 + BWI76_RS01950 0.46 -3.8 397,194 + BWI76_RS01950 0.46 -1.1 397,202 - BWI76_RS01950 0.47 -1.7 397,274 + BWI76_RS01950 0.49 -3.5 397,467 + BWI76_RS01950 0.56 -1.0 397,489 - BWI76_RS01950 0.57 -1.5 397,535 - BWI76_RS01950 0.58 -2.2 397,565 + BWI76_RS01950 0.59 -3.8 397,673 - BWI76_RS01950 0.63 -1.2 397,687 + BWI76_RS01950 0.64 +0.5 397,695 - BWI76_RS01950 0.64 -2.1 397,821 + +0.3 397,859 - BWI76_RS01950 0.70 -2.3 397,883 - BWI76_RS01950 0.71 -0.1 398,095 - BWI76_RS01950 0.78 +1.3 398,095 - BWI76_RS01950 0.78 -0.4 398,187 + BWI76_RS01950 0.81 -0.5 398,187 + BWI76_RS01950 0.81 -0.6 398,195 - BWI76_RS01950 0.82 -1.2 398,251 - BWI76_RS01950 0.84 -1.5 398,292 - BWI76_RS01950 0.85 -4.0 398,292 - BWI76_RS01950 0.85 -2.4 398,535 + -3.7 398,591 - -1.8 398,606 - -2.2 398,695 + -1.3 399,518 - -3.1
Or see this region's nucleotide sequence