Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01630

Experiment: K. M5a1 KLEB26 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01615 and BWI76_RS01620 are separated by 4 nucleotidesBWI76_RS01620 and BWI76_RS01625 are separated by 142 nucleotidesBWI76_RS01625 and BWI76_RS01630 are separated by 53 nucleotidesBWI76_RS01630 and BWI76_RS01635 are separated by 77 nucleotidesBWI76_RS01635 and BWI76_RS01640 are separated by 181 nucleotides BWI76_RS01615: BWI76_RS01615 - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/inosine monophosphate cyclohydrolase, at 327,829 to 329,418 _RS01615 BWI76_RS01620: BWI76_RS01620 - hypothetical protein, at 329,423 to 329,650 _RS01620 BWI76_RS01625: BWI76_RS01625 - hypothetical protein, at 329,793 to 329,998 _RS01625 BWI76_RS01630: BWI76_RS01630 - 16S ribosomal RNA, at 330,052 to 331,605 _RS01630 BWI76_RS01635: BWI76_RS01635 - tRNA-Glu, at 331,683 to 331,757 _RS01635 BWI76_RS01640: BWI76_RS01640 - 23S ribosomal RNA, at 331,939 to 334,865 _RS01640 Position (kb) 330 331 332Strain fitness (log2 ratio) -3 -2 -1 0 1at 329.130 kb on + strand, within BWI76_RS01615at 329.218 kb on - strand, within BWI76_RS01615at 329.306 kb on + strandat 329.312 kb on - strandat 329.368 kb on - strandat 329.428 kb on - strandat 329.594 kb on + strand, within BWI76_RS01620at 329.594 kb on + strand, within BWI76_RS01620at 329.600 kb on + strand, within BWI76_RS01620at 329.605 kb on + strand, within BWI76_RS01620at 330.515 kb on + strand, within BWI76_RS01630

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1
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329,130 + BWI76_RS01615 0.82 +1.0
329,218 - BWI76_RS01615 0.87 +0.1
329,306 + -1.2
329,312 - -1.6
329,368 - +0.0
329,428 - -2.2
329,594 + BWI76_RS01620 0.75 +0.7
329,594 + BWI76_RS01620 0.75 +0.1
329,600 + BWI76_RS01620 0.78 +0.8
329,605 + BWI76_RS01620 0.80 -1.6
330,515 + BWI76_RS01630 0.30 -3.6

Or see this region's nucleotide sequence