Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01100

Experiment: K. M5a1 KLEB26 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01095 and BWI76_RS01100 overlap by 4 nucleotidesBWI76_RS01100 and BWI76_RS01105 are separated by 18 nucleotidesBWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822 _RS01095 BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887 _RS01100 BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787 _RS01105 BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439 _RS01110 Position (kb) 226 227 228Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 226.034 kb on - strand, within BWI76_RS01095at 226.533 kb on + strand, within BWI76_RS01095at 226.541 kb on - strand, within BWI76_RS01095at 226.711 kb on + strandat 226.776 kb on + strandat 226.901 kb on + strandat 226.918 kb on + strandat 226.979 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.132 kb on + strand, within BWI76_RS01100at 227.140 kb on - strand, within BWI76_RS01100at 227.142 kb on + strand, within BWI76_RS01100at 227.227 kb on + strand, within BWI76_RS01100at 227.235 kb on - strand, within BWI76_RS01100at 227.642 kb on - strand, within BWI76_RS01100at 227.921 kb on - strandat 228.196 kb on - strand, within BWI76_RS01105at 228.249 kb on + strand, within BWI76_RS01105at 228.263 kb on - strand, within BWI76_RS01105at 228.273 kb on - strand, within BWI76_RS01105at 228.384 kb on + strand, within BWI76_RS01105at 228.654 kb on + strand, within BWI76_RS01105at 228.789 kb on - strandat 228.839 kb on - strand, within BWI76_RS01110

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB26 MOI 0.1
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226,034 - BWI76_RS01095 0.38 +4.1
226,533 + BWI76_RS01095 0.77 +3.7
226,541 - BWI76_RS01095 0.78 +4.1
226,711 + +3.7
226,776 + +4.0
226,901 + +1.1
226,918 + -1.2
226,979 + BWI76_RS01100 0.15 -2.2
227,132 + BWI76_RS01100 0.29 +1.7
227,132 + BWI76_RS01100 0.29 +1.7
227,132 + BWI76_RS01100 0.29 +1.2
227,140 - BWI76_RS01100 0.30 +2.6
227,142 + BWI76_RS01100 0.30 +0.8
227,227 + BWI76_RS01100 0.38 -0.3
227,235 - BWI76_RS01100 0.39 +2.8
227,642 - BWI76_RS01100 0.77 +1.6
227,921 - +0.0
228,196 - BWI76_RS01105 0.33 +2.1
228,249 + BWI76_RS01105 0.39 +1.1
228,263 - BWI76_RS01105 0.40 +2.3
228,273 - BWI76_RS01105 0.42 +2.3
228,384 + BWI76_RS01105 0.54 +1.4
228,654 + BWI76_RS01105 0.85 +1.3
228,789 - +2.1
228,839 - BWI76_RS01110 0.11 +2.9

Or see this region's nucleotide sequence