Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS00805

Experiment: Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS00795 and BPHYT_RS00800 are separated by 14 nucleotidesBPHYT_RS00800 and BPHYT_RS00805 overlap by 8 nucleotidesBPHYT_RS00805 and BPHYT_RS00810 overlap by 4 nucleotides BPHYT_RS00795: BPHYT_RS00795 - conjugal transfer protein TraJ, at 180,332 to 181,090 _RS00795 BPHYT_RS00800: BPHYT_RS00800 - Forms the bulk of type IV secretion complex that spans outer membrane and periplasm (VirB9), at 181,105 to 181,932 _RS00800 BPHYT_RS00805: BPHYT_RS00805 - type IV secretion system protein VirB10, at 181,925 to 183,127 _RS00805 BPHYT_RS00810: BPHYT_RS00810 - type IV secretion system protein VirB11, at 183,124 to 184,251 _RS00810 Position (kb) 181 182 183 184Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 181.005 kb on - strand, within BPHYT_RS00795at 181.142 kb on + strandat 181.143 kb on - strandat 181.171 kb on + strandat 181.172 kb on - strandat 181.366 kb on + strand, within BPHYT_RS00800at 181.366 kb on + strand, within BPHYT_RS00800at 181.367 kb on - strand, within BPHYT_RS00800at 181.430 kb on - strand, within BPHYT_RS00800at 181.489 kb on + strand, within BPHYT_RS00800at 181.490 kb on - strand, within BPHYT_RS00800at 181.490 kb on - strand, within BPHYT_RS00800at 181.597 kb on - strand, within BPHYT_RS00800at 181.603 kb on + strand, within BPHYT_RS00800at 181.676 kb on - strand, within BPHYT_RS00800at 182.182 kb on - strand, within BPHYT_RS00805at 182.242 kb on + strandat 182.292 kb on + strand, within BPHYT_RS00805at 182.339 kb on + strand, within BPHYT_RS00805at 182.340 kb on - strand, within BPHYT_RS00805at 182.340 kb on - strand, within BPHYT_RS00805at 182.343 kb on - strand, within BPHYT_RS00805at 182.635 kb on + strand, within BPHYT_RS00805at 182.844 kb on - strand, within BPHYT_RS00805at 182.844 kb on - strand, within BPHYT_RS00805at 182.958 kb on - strand, within BPHYT_RS00805at 182.958 kb on - strand, within BPHYT_RS00805at 183.098 kb on + strandat 183.099 kb on - strandat 183.099 kb on - strandat 183.171 kb on + strandat 183.172 kb on - strandat 183.638 kb on - strand, within BPHYT_RS00810at 183.697 kb on - strand, within BPHYT_RS00810at 183.714 kb on + strand, within BPHYT_RS00810at 183.844 kb on + strand, within BPHYT_RS00810at 183.845 kb on - strand, within BPHYT_RS00810at 183.845 kb on - strand, within BPHYT_RS00810at 183.959 kb on - strand, within BPHYT_RS00810at 184.009 kb on + strand, within BPHYT_RS00810at 184.010 kb on - strand, within BPHYT_RS00810

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
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181,005 - BPHYT_RS00795 0.89 -0.6
181,142 + +1.5
181,143 - +0.6
181,171 + +0.2
181,172 - +1.8
181,366 + BPHYT_RS00800 0.32 -0.1
181,366 + BPHYT_RS00800 0.32 +0.0
181,367 - BPHYT_RS00800 0.32 +1.0
181,430 - BPHYT_RS00800 0.39 -0.4
181,489 + BPHYT_RS00800 0.46 +0.8
181,490 - BPHYT_RS00800 0.46 +0.5
181,490 - BPHYT_RS00800 0.46 +0.3
181,597 - BPHYT_RS00800 0.59 -0.1
181,603 + BPHYT_RS00800 0.60 -0.0
181,676 - BPHYT_RS00800 0.69 +0.5
182,182 - BPHYT_RS00805 0.21 -0.5
182,242 + +3.5
182,292 + BPHYT_RS00805 0.31 +0.8
182,339 + BPHYT_RS00805 0.34 +0.3
182,340 - BPHYT_RS00805 0.34 -0.4
182,340 - BPHYT_RS00805 0.34 -0.4
182,343 - BPHYT_RS00805 0.35 +0.1
182,635 + BPHYT_RS00805 0.59 -0.2
182,844 - BPHYT_RS00805 0.76 +0.1
182,844 - BPHYT_RS00805 0.76 +1.1
182,958 - BPHYT_RS00805 0.86 -1.4
182,958 - BPHYT_RS00805 0.86 +0.5
183,098 + +0.1
183,099 - +3.0
183,099 - -0.3
183,171 + +0.3
183,172 - +1.1
183,638 - BPHYT_RS00810 0.46 -1.5
183,697 - BPHYT_RS00810 0.51 -0.5
183,714 + BPHYT_RS00810 0.52 -0.1
183,844 + BPHYT_RS00810 0.64 +0.2
183,845 - BPHYT_RS00810 0.64 -0.3
183,845 - BPHYT_RS00810 0.64 +1.1
183,959 - BPHYT_RS00810 0.74 +0.2
184,009 + BPHYT_RS00810 0.78 -3.0
184,010 - BPHYT_RS00810 0.79 -0.0

Or see this region's nucleotide sequence