Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27625

Experiment: B3 MOi 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27615 and BWI76_RS27620 are separated by 185 nucleotidesBWI76_RS27620 and BWI76_RS27625 are separated by 112 nucleotidesBWI76_RS27625 and BWI76_RS27630 are separated by 1 nucleotidesBWI76_RS27630 and BWI76_RS27635 overlap by 4 nucleotides BWI76_RS27615: BWI76_RS27615 - putative Shikimate kinase, at 5,731,743 to 5,732,348 _RS27615 BWI76_RS27620: BWI76_RS27620 - hypothetical protein, at 5,732,534 to 5,733,001 _RS27620 BWI76_RS27625: BWI76_RS27625 - NADPH:quinone reductase, at 5,733,114 to 5,734,121 _RS27625 BWI76_RS27630: BWI76_RS27630 - antibiotic biosynthesis monooxygenase, at 5,734,123 to 5,734,461 _RS27630 BWI76_RS27635: BWI76_RS27635 - AraC family transcriptional regulator, at 5,734,458 to 5,735,411 _RS27635 Position (kb) 5733 5734 5735Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5732.153 kb on + strand, within BWI76_RS27615at 5732.178 kb on - strand, within BWI76_RS27615at 5732.280 kb on - strand, within BWI76_RS27615at 5732.294 kb on + strandat 5732.348 kb on + strandat 5732.356 kb on - strandat 5732.356 kb on - strandat 5732.356 kb on - strandat 5732.356 kb on - strandat 5732.374 kb on + strandat 5732.432 kb on + strandat 5732.440 kb on - strandat 5732.460 kb on - strandat 5732.492 kb on - strandat 5732.568 kb on + strandat 5732.576 kb on - strandat 5732.576 kb on - strandat 5732.587 kb on + strand, within BWI76_RS27620at 5732.624 kb on - strand, within BWI76_RS27620at 5732.631 kb on + strand, within BWI76_RS27620at 5732.639 kb on - strand, within BWI76_RS27620at 5732.639 kb on - strand, within BWI76_RS27620at 5732.925 kb on - strand, within BWI76_RS27620at 5732.956 kb on - strandat 5733.066 kb on + strandat 5733.069 kb on - strandat 5733.070 kb on - strandat 5733.112 kb on + strandat 5733.389 kb on + strand, within BWI76_RS27625at 5733.469 kb on - strand, within BWI76_RS27625at 5733.532 kb on + strand, within BWI76_RS27625at 5733.830 kb on + strand, within BWI76_RS27625at 5733.870 kb on - strand, within BWI76_RS27625at 5733.905 kb on - strand, within BWI76_RS27625at 5733.924 kb on + strand, within BWI76_RS27625at 5734.115 kb on + strandat 5734.123 kb on - strandat 5734.160 kb on + strand, within BWI76_RS27630at 5734.163 kb on - strand, within BWI76_RS27630at 5734.163 kb on - strand, within BWI76_RS27630at 5734.163 kb on - strand, within BWI76_RS27630at 5734.307 kb on + strand, within BWI76_RS27630at 5734.436 kb on + strandat 5734.477 kb on + strandat 5734.498 kb on - strandat 5734.533 kb on + strandat 5734.567 kb on - strand, within BWI76_RS27635at 5734.585 kb on + strand, within BWI76_RS27635at 5735.074 kb on - strand, within BWI76_RS27635at 5735.090 kb on + strand, within BWI76_RS27635

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Per-strain Table

Position Strand Gene LocusTag Fraction B3 MOi 1
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5,732,153 + BWI76_RS27615 0.68 +1.6
5,732,178 - BWI76_RS27615 0.72 -0.3
5,732,280 - BWI76_RS27615 0.89 +0.2
5,732,294 + -1.5
5,732,348 + +0.7
5,732,356 - -1.5
5,732,356 - -0.5
5,732,356 - +1.6
5,732,356 - -0.1
5,732,374 + +0.1
5,732,432 + -0.4
5,732,440 - +0.1
5,732,460 - +0.4
5,732,492 - +0.3
5,732,568 + -0.1
5,732,576 - -1.7
5,732,576 - +0.7
5,732,587 + BWI76_RS27620 0.11 -1.2
5,732,624 - BWI76_RS27620 0.19 -0.6
5,732,631 + BWI76_RS27620 0.21 -0.4
5,732,639 - BWI76_RS27620 0.22 +0.4
5,732,639 - BWI76_RS27620 0.22 +0.7
5,732,925 - BWI76_RS27620 0.84 +1.8
5,732,956 - +0.6
5,733,066 + +0.8
5,733,069 - -0.5
5,733,070 - +0.1
5,733,112 + -0.4
5,733,389 + BWI76_RS27625 0.27 -0.4
5,733,469 - BWI76_RS27625 0.35 -0.3
5,733,532 + BWI76_RS27625 0.41 -0.9
5,733,830 + BWI76_RS27625 0.71 -1.1
5,733,870 - BWI76_RS27625 0.75 -0.4
5,733,905 - BWI76_RS27625 0.78 -1.1
5,733,924 + BWI76_RS27625 0.80 +0.2
5,734,115 + +0.7
5,734,123 - -0.8
5,734,160 + BWI76_RS27630 0.11 -1.6
5,734,163 - BWI76_RS27630 0.12 -0.3
5,734,163 - BWI76_RS27630 0.12 -0.2
5,734,163 - BWI76_RS27630 0.12 -0.3
5,734,307 + BWI76_RS27630 0.54 +2.4
5,734,436 + -0.1
5,734,477 + -0.0
5,734,498 - +1.9
5,734,533 + +0.2
5,734,567 - BWI76_RS27635 0.11 +0.2
5,734,585 + BWI76_RS27635 0.13 -0.5
5,735,074 - BWI76_RS27635 0.65 -1.8
5,735,090 + BWI76_RS27635 0.66 +3.5

Or see this region's nucleotide sequence