Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24580 and BWI76_RS24585 are separated by 106 nucleotides BWI76_RS24585 and BWI76_RS24590 are separated by 357 nucleotides BWI76_RS24590 and BWI76_RS24595 overlap by 4 nucleotides
BWI76_RS24580: BWI76_RS24580 - PTS-dependent dihydroxyacetone kinase operon transcriptional regulator DhaR, at 5,122,692 to 5,124,605
_RS24580
BWI76_RS24585: BWI76_RS24585 - glycerol dehydrogenase, at 5,124,712 to 5,125,809
_RS24585
BWI76_RS24590: BWI76_RS24590 - hypothetical protein, at 5,126,167 to 5,126,409
_RS24590
BWI76_RS24595: BWI76_RS24595 - dihydroxyacetone kinase subunit DhaK, at 5,126,406 to 5,127,476
_RS24595
Position (kb) 5124
5125
5126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5123.978 kb on + strand, within BWI76_RS24580 at 5124.083 kb on + strand, within BWI76_RS24580 at 5124.281 kb on - strand, within BWI76_RS24580 at 5124.332 kb on + strand, within BWI76_RS24580 at 5124.380 kb on + strand, within BWI76_RS24580 at 5124.466 kb on + strand at 5124.569 kb on - strand at 5124.609 kb on - strand at 5124.705 kb on + strand at 5124.713 kb on - strand at 5124.730 kb on - strand at 5124.749 kb on - strand at 5124.798 kb on - strand at 5124.972 kb on + strand, within BWI76_RS24585 at 5125.136 kb on + strand, within BWI76_RS24585 at 5125.429 kb on - strand, within BWI76_RS24585 at 5125.438 kb on + strand, within BWI76_RS24585 at 5125.467 kb on + strand, within BWI76_RS24585 at 5125.649 kb on - strand, within BWI76_RS24585 at 5125.704 kb on - strand at 5125.826 kb on - strand at 5125.829 kb on + strand at 5125.920 kb on + strand at 5126.439 kb on - strand at 5126.450 kb on + strand at 5126.725 kb on - strand, within BWI76_RS24595 at 5126.783 kb on + strand, within BWI76_RS24595
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 5,123,978 + BWI76_RS24580 0.67 -0.3 5,124,083 + BWI76_RS24580 0.73 +1.1 5,124,281 - BWI76_RS24580 0.83 +1.9 5,124,332 + BWI76_RS24580 0.86 -0.6 5,124,380 + BWI76_RS24580 0.88 +0.9 5,124,466 + +0.5 5,124,569 - +0.4 5,124,609 - +0.2 5,124,705 + -0.6 5,124,713 - -0.3 5,124,730 - -1.0 5,124,749 - -1.7 5,124,798 - -0.5 5,124,972 + BWI76_RS24585 0.24 -3.3 5,125,136 + BWI76_RS24585 0.39 -1.9 5,125,429 - BWI76_RS24585 0.65 +0.8 5,125,438 + BWI76_RS24585 0.66 -1.7 5,125,467 + BWI76_RS24585 0.69 +0.1 5,125,649 - BWI76_RS24585 0.85 -1.1 5,125,704 - +0.5 5,125,826 - -0.5 5,125,829 + +1.7 5,125,920 + +0.0 5,126,439 - -0.1 5,126,450 + -0.5 5,126,725 - BWI76_RS24595 0.30 +0.8 5,126,783 + BWI76_RS24595 0.35 -0.6
Or see this region's nucleotide sequence