Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20375

Experiment: B3 MOi 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20370 and BWI76_RS20375 are separated by 131 nucleotidesBWI76_RS20375 and BWI76_RS20380 are separated by 27 nucleotidesBWI76_RS20380 and BWI76_RS20385 overlap by 1 nucleotides BWI76_RS20370: BWI76_RS20370 - acetyl-CoA carboxylase carboxyl transferase subunit beta, at 4,264,098 to 4,265,075 _RS20370 BWI76_RS20375: BWI76_RS20375 - membrane protein, at 4,265,207 to 4,265,866 _RS20375 BWI76_RS20380: BWI76_RS20380 - tRNA pseudouridine(38-40) synthase TruA, at 4,265,894 to 4,266,706 _RS20380 BWI76_RS20385: BWI76_RS20385 - aspartate-semialdehyde dehydrogenase, at 4,266,706 to 4,267,719 _RS20385 Position (kb) 4265 4266Strain fitness (log2 ratio) -1 0 1at 4265.404 kb on + strand, within BWI76_RS20375at 4265.756 kb on - strand, within BWI76_RS20375at 4265.878 kb on + strandat 4265.909 kb on - strandat 4266.308 kb on + strand, within BWI76_RS20380at 4266.788 kb on + strandat 4266.793 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction B3 MOi 1
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4,265,404 + BWI76_RS20375 0.30 -1.7
4,265,756 - BWI76_RS20375 0.83 +1.5
4,265,878 + -0.3
4,265,909 - +1.0
4,266,308 + BWI76_RS20380 0.51 -0.4
4,266,788 + +0.6
4,266,793 + -0.1

Or see this region's nucleotide sequence