Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13210 and BWI76_RS13215 are separated by 295 nucleotides BWI76_RS13215 and BWI76_RS13220 are separated by 243 nucleotides BWI76_RS13220 and BWI76_RS13225 are separated by 86 nucleotides
BWI76_RS13210: BWI76_RS13210 - aldehyde dehydrogenase, at 2,756,501 to 2,757,940
_RS13210
BWI76_RS13215: BWI76_RS13215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 2,758,236 to 2,759,234
_RS13215
BWI76_RS13220: BWI76_RS13220 - N-acetyltransferase, at 2,759,478 to 2,759,972
_RS13220
BWI76_RS13225: BWI76_RS13225 - hypothetical protein, at 2,760,059 to 2,760,985
_RS13225
Position (kb)
2758
2759
2760 Strain fitness (log2 ratio)
-1
0
1
2 at 2757.535 kb on + strand, within BWI76_RS13210 at 2757.580 kb on - strand, within BWI76_RS13210 at 2757.607 kb on + strand, within BWI76_RS13210 at 2757.664 kb on + strand, within BWI76_RS13210 at 2757.687 kb on + strand, within BWI76_RS13210 at 2758.039 kb on - strand at 2758.087 kb on + strand at 2758.335 kb on - strand at 2758.950 kb on - strand, within BWI76_RS13215 at 2759.150 kb on + strand at 2759.150 kb on + strand at 2759.153 kb on + strand at 2759.158 kb on - strand at 2759.204 kb on + strand at 2759.454 kb on + strand at 2759.524 kb on - strand at 2759.681 kb on - strand, within BWI76_RS13220 at 2759.835 kb on + strand, within BWI76_RS13220 at 2760.021 kb on - strand at 2760.034 kb on - strand at 2760.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 2,757,535 + BWI76_RS13210 0.72 +1.0 2,757,580 - BWI76_RS13210 0.75 -0.7 2,757,607 + BWI76_RS13210 0.77 -0.1 2,757,664 + BWI76_RS13210 0.81 +0.4 2,757,687 + BWI76_RS13210 0.82 +0.3 2,758,039 - +0.8 2,758,087 + +2.5 2,758,335 - -0.2 2,758,950 - BWI76_RS13215 0.71 -0.5 2,759,150 + +0.0 2,759,150 + +0.7 2,759,153 + +1.2 2,759,158 - +0.1 2,759,204 + -0.7 2,759,454 + -0.8 2,759,524 - +2.2 2,759,681 - BWI76_RS13220 0.41 +0.2 2,759,835 + BWI76_RS13220 0.72 -0.2 2,760,021 - +1.1 2,760,034 - -0.5 2,760,103 - -0.4
Or see this region's nucleotide sequence