Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12135 and BWI76_RS12140 are separated by 248 nucleotides BWI76_RS12140 and BWI76_RS12145 overlap by 1 nucleotides BWI76_RS12145 and BWI76_RS12150 are separated by 550 nucleotides BWI76_RS12150 and BWI76_RS12155 are separated by 78 nucleotides
BWI76_RS12135: BWI76_RS12135 - C-5 cytosine-specific DNA methylase, at 2,543,775 to 2,545,757
_RS12135
BWI76_RS12140: BWI76_RS12140 - hypothetical protein, at 2,546,006 to 2,546,659
_RS12140
BWI76_RS12145: BWI76_RS12145 - hypothetical protein, at 2,546,659 to 2,547,765
_RS12145
BWI76_RS12150: BWI76_RS12150 - DNA damage-inducible protein I, at 2,548,316 to 2,548,549
_RS12150
BWI76_RS12155: BWI76_RS12155 - hypothetical protein, at 2,548,628 to 2,548,849
_RS12155
Position (kb)
2546
2547
2548 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.672 kb on + strand at 2545.680 kb on - strand at 2545.681 kb on - strand at 2545.681 kb on - strand at 2545.704 kb on + strand at 2545.709 kb on + strand at 2545.709 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.714 kb on + strand at 2545.717 kb on - strand at 2545.722 kb on - strand at 2545.728 kb on + strand at 2545.733 kb on + strand at 2545.738 kb on + strand at 2545.738 kb on + strand at 2545.741 kb on - strand at 2545.741 kb on - strand at 2545.746 kb on - strand at 2545.786 kb on + strand at 2545.811 kb on + strand at 2545.814 kb on - strand at 2545.814 kb on - strand at 2545.819 kb on - strand at 2545.824 kb on + strand at 2545.832 kb on - strand at 2545.840 kb on - strand at 2545.886 kb on + strand at 2545.886 kb on + strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.894 kb on - strand at 2545.925 kb on - strand at 2545.930 kb on - strand at 2545.930 kb on - strand at 2545.930 kb on - strand at 2546.823 kb on + strand, within BWI76_RS12145 at 2546.914 kb on - strand, within BWI76_RS12145 at 2547.568 kb on - strand, within BWI76_RS12145 at 2547.568 kb on - strand, within BWI76_RS12145 at 2547.788 kb on + strand at 2547.875 kb on + strand at 2547.879 kb on + strand at 2547.883 kb on + strand at 2547.884 kb on + strand at 2547.902 kb on - strand at 2547.909 kb on + strand at 2547.909 kb on + strand at 2547.909 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.944 kb on + strand at 2547.959 kb on - strand at 2547.963 kb on - strand at 2547.969 kb on + strand at 2547.972 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.980 kb on - strand at 2547.985 kb on - strand at 2547.985 kb on - strand at 2547.990 kb on + strand at 2548.004 kb on - strand at 2548.004 kb on - strand at 2548.145 kb on + strand at 2548.145 kb on + strand at 2548.153 kb on - strand at 2548.306 kb on + strand at 2548.362 kb on + strand, within BWI76_RS12150 at 2548.373 kb on + strand, within BWI76_RS12150 at 2548.456 kb on + strand, within BWI76_RS12150 at 2548.457 kb on - strand, within BWI76_RS12150 at 2548.464 kb on - strand, within BWI76_RS12150 at 2548.471 kb on - strand, within BWI76_RS12150 at 2548.587 kb on + strand at 2548.608 kb on - strand at 2548.687 kb on - strand, within BWI76_RS12155 at 2548.687 kb on - strand, within BWI76_RS12155
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 2,545,672 + -0.2 2,545,672 + +0.0 2,545,672 + +1.6 2,545,672 + +0.7 2,545,680 - -1.5 2,545,681 - +0.9 2,545,681 - +0.5 2,545,704 + -0.0 2,545,709 + +0.3 2,545,709 + -2.1 2,545,714 + +0.2 2,545,714 + -1.8 2,545,714 + -1.0 2,545,714 + -0.5 2,545,714 + +0.4 2,545,717 - -1.1 2,545,722 - -0.8 2,545,728 + -0.3 2,545,733 + -0.5 2,545,738 + -0.2 2,545,738 + +0.2 2,545,741 - -2.0 2,545,741 - +0.5 2,545,746 - -0.9 2,545,786 + -0.5 2,545,811 + +0.3 2,545,814 - -0.2 2,545,814 - +0.3 2,545,819 - +0.2 2,545,824 + +1.5 2,545,832 - -1.4 2,545,840 - +0.3 2,545,886 + +1.2 2,545,886 + +0.7 2,545,894 - +0.3 2,545,894 - -0.5 2,545,894 - +0.6 2,545,894 - +0.5 2,545,894 - +1.5 2,545,894 - +2.2 2,545,894 - +0.0 2,545,925 - -1.4 2,545,930 - -0.1 2,545,930 - +1.6 2,545,930 - +0.3 2,546,823 + BWI76_RS12145 0.15 -2.0 2,546,914 - BWI76_RS12145 0.23 -0.0 2,547,568 - BWI76_RS12145 0.82 +1.2 2,547,568 - BWI76_RS12145 0.82 +1.2 2,547,788 + -1.0 2,547,875 + +0.6 2,547,879 + -0.5 2,547,883 + -1.0 2,547,884 + +1.2 2,547,902 - -0.8 2,547,909 + +0.2 2,547,909 + -0.2 2,547,909 + -0.2 2,547,920 + +0.7 2,547,920 + +0.0 2,547,920 + +0.3 2,547,920 + -0.1 2,547,920 + -0.8 2,547,920 + +0.9 2,547,920 + -0.2 2,547,920 + +0.3 2,547,920 + +0.5 2,547,920 + -1.1 2,547,920 + +1.1 2,547,920 + +0.4 2,547,920 + +0.9 2,547,920 + +3.8 2,547,920 + +1.5 2,547,928 - -1.1 2,547,928 - +0.8 2,547,928 - -2.4 2,547,928 - -0.5 2,547,928 - +0.2 2,547,928 - +0.3 2,547,944 + +0.4 2,547,959 - -0.9 2,547,963 - +0.2 2,547,969 + -0.2 2,547,972 + +1.8 2,547,977 + -0.5 2,547,977 + +0.2 2,547,977 + -1.0 2,547,977 + +0.2 2,547,977 + -1.8 2,547,980 - -0.6 2,547,985 - -0.3 2,547,985 - -2.0 2,547,990 + -0.2 2,548,004 - +0.2 2,548,004 - -2.5 2,548,145 + -0.0 2,548,145 + -0.6 2,548,153 - +1.5 2,548,306 + -0.3 2,548,362 + BWI76_RS12150 0.20 +0.4 2,548,373 + BWI76_RS12150 0.24 +0.7 2,548,456 + BWI76_RS12150 0.60 -0.4 2,548,457 - BWI76_RS12150 0.60 +2.0 2,548,464 - BWI76_RS12150 0.63 -1.6 2,548,471 - BWI76_RS12150 0.66 -1.1 2,548,587 + -1.1 2,548,608 - +1.2 2,548,687 - BWI76_RS12155 0.27 +1.2 2,548,687 - BWI76_RS12155 0.27 -0.4
Or see this region's nucleotide sequence