Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10465 and BWI76_RS10470 are separated by 261 nucleotides BWI76_RS10470 and BWI76_RS10475 are separated by 23 nucleotides
BWI76_RS10465: BWI76_RS10465 - hypothetical protein, at 2,210,969 to 2,211,493
_RS10465
BWI76_RS10470: BWI76_RS10470 - iron ABC transporter substrate-binding protein, at 2,211,755 to 2,212,771
_RS10470
BWI76_RS10475: BWI76_RS10475 - binding-protein-dependent transport system inner membrane protein, at 2,212,795 to 2,214,369
_RS10475
Position (kb)
2211
2212
2213 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2210.910 kb on - strand at 2210.924 kb on - strand at 2210.939 kb on + strand at 2210.940 kb on + strand at 2210.945 kb on + strand at 2210.953 kb on - strand at 2210.958 kb on - strand at 2210.963 kb on + strand at 2210.963 kb on + strand at 2210.966 kb on - strand at 2210.971 kb on - strand at 2210.971 kb on - strand at 2210.971 kb on - strand at 2210.988 kb on + strand at 2210.988 kb on + strand at 2211.011 kb on + strand at 2211.119 kb on + strand, within BWI76_RS10465 at 2211.192 kb on - strand, within BWI76_RS10465 at 2211.288 kb on + strand, within BWI76_RS10465 at 2211.322 kb on + strand, within BWI76_RS10465 at 2211.330 kb on - strand, within BWI76_RS10465 at 2211.383 kb on - strand, within BWI76_RS10465 at 2211.467 kb on - strand at 2211.488 kb on + strand at 2211.491 kb on - strand at 2211.500 kb on + strand at 2211.501 kb on + strand at 2211.501 kb on + strand at 2211.501 kb on + strand at 2211.508 kb on - strand at 2211.508 kb on - strand at 2211.508 kb on - strand at 2211.509 kb on - strand at 2211.509 kb on - strand at 2211.509 kb on - strand at 2211.601 kb on + strand at 2211.612 kb on + strand at 2211.612 kb on + strand at 2211.612 kb on + strand at 2211.616 kb on + strand at 2211.620 kb on - strand at 2211.682 kb on - strand at 2211.693 kb on + strand at 2211.701 kb on - strand at 2211.718 kb on + strand at 2211.718 kb on + strand at 2211.718 kb on + strand at 2211.718 kb on + strand at 2211.724 kb on + strand at 2211.726 kb on - strand at 2211.726 kb on - strand at 2211.728 kb on - strand at 2211.738 kb on + strand at 2211.738 kb on + strand at 2211.738 kb on + strand at 2211.745 kb on + strand at 2211.746 kb on - strand at 2211.753 kb on - strand at 2211.929 kb on - strand, within BWI76_RS10470 at 2211.937 kb on + strand, within BWI76_RS10470 at 2211.945 kb on - strand, within BWI76_RS10470 at 2212.080 kb on + strand, within BWI76_RS10470 at 2212.687 kb on + strand at 2212.801 kb on + strand at 2212.889 kb on + strand at 2213.006 kb on + strand, within BWI76_RS10475 at 2213.299 kb on + strand, within BWI76_RS10475 at 2213.357 kb on - strand, within BWI76_RS10475
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 2,210,910 - +3.2 2,210,924 - +0.6 2,210,939 + -0.1 2,210,940 + -0.1 2,210,945 + -2.5 2,210,953 - +0.1 2,210,958 - -1.2 2,210,963 + -0.4 2,210,963 + -0.7 2,210,966 - +0.2 2,210,971 - -1.8 2,210,971 - -1.8 2,210,971 - -0.7 2,210,988 + -1.6 2,210,988 + +0.2 2,211,011 + -0.4 2,211,119 + BWI76_RS10465 0.29 +0.7 2,211,192 - BWI76_RS10465 0.42 -2.7 2,211,288 + BWI76_RS10465 0.61 +0.2 2,211,322 + BWI76_RS10465 0.67 -2.3 2,211,330 - BWI76_RS10465 0.69 -0.8 2,211,383 - BWI76_RS10465 0.79 +2.2 2,211,467 - -1.1 2,211,488 + -0.1 2,211,491 - +3.0 2,211,500 + +1.4 2,211,501 + -1.6 2,211,501 + +0.7 2,211,501 + +1.2 2,211,508 - +3.5 2,211,508 - +0.7 2,211,508 - -1.6 2,211,509 - +0.1 2,211,509 - -0.6 2,211,509 - -0.6 2,211,601 + -0.6 2,211,612 + +1.4 2,211,612 + +0.2 2,211,612 + -0.6 2,211,616 + -0.7 2,211,620 - +0.8 2,211,682 - -0.6 2,211,693 + +0.4 2,211,701 - -0.4 2,211,718 + -1.4 2,211,718 + -1.4 2,211,718 + -0.1 2,211,718 + -0.0 2,211,724 + -2.0 2,211,726 - -0.4 2,211,726 - +0.7 2,211,728 - -1.0 2,211,738 + +1.3 2,211,738 + -0.1 2,211,738 + +0.4 2,211,745 + -0.2 2,211,746 - -1.3 2,211,753 - -0.7 2,211,929 - BWI76_RS10470 0.17 +1.2 2,211,937 + BWI76_RS10470 0.18 +0.3 2,211,945 - BWI76_RS10470 0.19 -0.8 2,212,080 + BWI76_RS10470 0.32 -1.3 2,212,687 + +0.1 2,212,801 + +0.3 2,212,889 + -0.6 2,213,006 + BWI76_RS10475 0.13 +1.0 2,213,299 + BWI76_RS10475 0.32 +0.8 2,213,357 - BWI76_RS10475 0.36 +1.6
Or see this region's nucleotide sequence