Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10055 and BWI76_RS10060 are separated by 51 nucleotides BWI76_RS10060 and BWI76_RS10065 overlap by 4 nucleotides BWI76_RS10065 and BWI76_RS10070 are separated by 89 nucleotides BWI76_RS10070 and BWI76_RS10075 are separated by 267 nucleotides
BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074
_RS10055
BWI76_RS10060: BWI76_RS10060 - acylphosphatase, at 2,131,126 to 2,131,407
_RS10060
BWI76_RS10065: BWI76_RS10065 - sulfurtransferase TusE, at 2,131,404 to 2,131,733
_RS10065
BWI76_RS10070: BWI76_RS10070 - BAX inhibitor protein, at 2,131,823 to 2,132,482
_RS10070
BWI76_RS10075: BWI76_RS10075 - peptide ABC transporter substrate-binding protein, at 2,132,750 to 2,134,378
_RS10075
Position (kb)
2131
2132
2133 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2130.866 kb on + strand, within BWI76_RS10055 at 2131.088 kb on + strand at 2131.358 kb on + strand, within BWI76_RS10060 at 2132.155 kb on + strand, within BWI76_RS10070 at 2132.211 kb on - strand, within BWI76_RS10070 at 2132.355 kb on + strand, within BWI76_RS10070 at 2132.389 kb on + strand, within BWI76_RS10070 at 2132.551 kb on + strand at 2132.561 kb on + strand at 2132.561 kb on + strand at 2132.571 kb on + strand at 2132.707 kb on + strand at 2132.715 kb on - strand at 2132.715 kb on - strand at 2132.717 kb on + strand at 2132.717 kb on + strand at 2132.999 kb on - strand, within BWI76_RS10075 at 2133.201 kb on - strand, within BWI76_RS10075 at 2133.256 kb on - strand, within BWI76_RS10075 at 2133.258 kb on + strand, within BWI76_RS10075 at 2133.266 kb on - strand, within BWI76_RS10075
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 2,130,866 + BWI76_RS10055 0.74 -2.0 2,131,088 + +2.1 2,131,358 + BWI76_RS10060 0.82 +0.2 2,132,155 + BWI76_RS10070 0.50 -0.6 2,132,211 - BWI76_RS10070 0.59 -0.5 2,132,355 + BWI76_RS10070 0.81 -1.5 2,132,389 + BWI76_RS10070 0.86 -0.9 2,132,551 + -1.1 2,132,561 + -0.5 2,132,561 + +1.0 2,132,571 + -0.4 2,132,707 + -0.3 2,132,715 - -1.1 2,132,715 - +2.1 2,132,717 + -0.3 2,132,717 + -0.1 2,132,999 - BWI76_RS10075 0.15 +0.3 2,133,201 - BWI76_RS10075 0.28 -0.6 2,133,256 - BWI76_RS10075 0.31 -0.7 2,133,258 + BWI76_RS10075 0.31 +0.9 2,133,266 - BWI76_RS10075 0.32 +0.1
Or see this region's nucleotide sequence