Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08370 and BWI76_RS08375 are separated by 9 nucleotides BWI76_RS08375 and BWI76_RS08380 are separated by 15 nucleotides BWI76_RS08380 and BWI76_RS08385 are separated by 45 nucleotides
BWI76_RS08370: BWI76_RS08370 - membrane protein, at 1,771,176 to 1,772,171
_RS08370
BWI76_RS08375: BWI76_RS08375 - pyroglutamyl-peptidase I, at 1,772,181 to 1,772,825
_RS08375
BWI76_RS08380: BWI76_RS08380 - endonuclease VIII, at 1,772,841 to 1,773,632
_RS08380
BWI76_RS08385: BWI76_RS08385 - citrate (Si)-synthase, at 1,773,678 to 1,774,961
_RS08385
Position (kb) 1772
1773 Strain fitness (log2 ratio) -1
0
1 at 1771.234 kb on - strand at 1771.286 kb on + strand, within BWI76_RS08370 at 1771.330 kb on + strand, within BWI76_RS08370 at 1772.016 kb on + strand, within BWI76_RS08370 at 1772.108 kb on + strand at 1772.116 kb on + strand at 1772.627 kb on - strand, within BWI76_RS08375 at 1772.633 kb on + strand, within BWI76_RS08375 at 1772.904 kb on + strand at 1772.912 kb on - strand at 1773.069 kb on + strand, within BWI76_RS08380 at 1773.220 kb on + strand, within BWI76_RS08380 at 1773.794 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 1,771,234 - -1.4 1,771,286 + BWI76_RS08370 0.11 -0.4 1,771,330 + BWI76_RS08370 0.15 +0.9 1,772,016 + BWI76_RS08370 0.84 +0.1 1,772,108 + -0.4 1,772,116 + -0.2 1,772,627 - BWI76_RS08375 0.69 -0.8 1,772,633 + BWI76_RS08375 0.70 -0.7 1,772,904 + +0.5 1,772,912 - +0.2 1,773,069 + BWI76_RS08380 0.29 -0.8 1,773,220 + BWI76_RS08380 0.48 +0.7 1,773,794 + +1.7
Or see this region's nucleotide sequence