Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06375

Experiment: B3 MOi 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06365 and BWI76_RS06370 overlap by 14 nucleotidesBWI76_RS06370 and BWI76_RS06375 are separated by 289 nucleotidesBWI76_RS06375 and BWI76_RS06380 are separated by 60 nucleotides BWI76_RS06365: BWI76_RS06365 - thiamine-monophosphate kinase, at 1,337,814 to 1,338,791 _RS06365 BWI76_RS06370: BWI76_RS06370 - phosphatidylglycerophosphatase A, at 1,338,778 to 1,339,284 _RS06370 BWI76_RS06375: BWI76_RS06375 - oxidoreductase, at 1,339,574 to 1,340,548 _RS06375 BWI76_RS06380: BWI76_RS06380 - 1-deoxy-D-xylulose-5-phosphate synthase, at 1,340,609 to 1,342,471 _RS06380 Position (kb) 1339 1340 1341Strain fitness (log2 ratio) -1 0 1at 1338.855 kb on - strand, within BWI76_RS06370at 1338.982 kb on - strand, within BWI76_RS06370at 1339.011 kb on - strand, within BWI76_RS06370at 1339.050 kb on + strand, within BWI76_RS06370at 1339.222 kb on + strand, within BWI76_RS06370at 1339.255 kb on + strandat 1339.260 kb on + strandat 1339.398 kb on - strandat 1339.404 kb on - strandat 1339.426 kb on + strandat 1339.523 kb on - strandat 1339.611 kb on + strandat 1339.630 kb on + strandat 1339.718 kb on + strand, within BWI76_RS06375at 1339.739 kb on - strand, within BWI76_RS06375at 1340.120 kb on + strand, within BWI76_RS06375at 1340.586 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction B3 MOi 1
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1,338,855 - BWI76_RS06370 0.15 -0.4
1,338,982 - BWI76_RS06370 0.40 +0.3
1,339,011 - BWI76_RS06370 0.46 -1.4
1,339,050 + BWI76_RS06370 0.54 -0.7
1,339,222 + BWI76_RS06370 0.88 -0.2
1,339,255 + -0.6
1,339,260 + +0.5
1,339,398 - +0.2
1,339,404 - -0.1
1,339,426 + -0.4
1,339,523 - -0.0
1,339,611 + +0.3
1,339,630 + +0.2
1,339,718 + BWI76_RS06375 0.15 +0.2
1,339,739 - BWI76_RS06375 0.17 -0.4
1,340,120 + BWI76_RS06375 0.56 -0.9
1,340,586 - -0.5

Or see this region's nucleotide sequence