Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01555 and BWI76_RS01560 are separated by 253 nucleotides BWI76_RS01560 and BWI76_RS01565 are separated by 95 nucleotides BWI76_RS01565 and BWI76_RS01570 are separated by 40 nucleotides
BWI76_RS01555: BWI76_RS01555 - phosphomethylpyrimidine synthase ThiC, at 316,023 to 317,918
_RS01555
BWI76_RS01560: BWI76_RS01560 - Rsd/AlgQ family anti-sigma factor, at 318,172 to 318,669
_RS01560
BWI76_RS01565: BWI76_RS01565 - NADH pyrophosphatase, at 318,765 to 319,538
_RS01565
BWI76_RS01570: BWI76_RS01570 - uroporphyrinogen decarboxylase, at 319,579 to 320,643
_RS01570
Position (kb) 318
319
320 Strain fitness (log2 ratio) -1
0
1 at 317.813 kb on + strand at 317.849 kb on + strand at 317.946 kb on - strand at 317.990 kb on - strand at 318.007 kb on + strand at 318.040 kb on - strand at 318.040 kb on - strand at 318.142 kb on + strand at 318.176 kb on - strand at 318.199 kb on + strand at 318.215 kb on - strand at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.371 kb on + strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.379 kb on - strand, within BWI76_RS01560 at 318.483 kb on - strand, within BWI76_RS01560 at 318.564 kb on + strand, within BWI76_RS01560 at 318.605 kb on - strand, within BWI76_RS01560 at 318.630 kb on + strand at 318.638 kb on - strand at 318.668 kb on - strand at 318.754 kb on + strand at 319.333 kb on + strand, within BWI76_RS01565 at 319.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 317,813 + +0.1 317,849 + +0.2 317,946 - -0.2 317,990 - -0.0 318,007 + -0.3 318,040 - -0.0 318,040 - +0.9 318,142 + +0.3 318,176 - +0.7 318,199 + +0.1 318,215 - +0.5 318,371 + BWI76_RS01560 0.40 +0.6 318,371 + BWI76_RS01560 0.40 +1.6 318,371 + BWI76_RS01560 0.40 +0.5 318,379 - BWI76_RS01560 0.42 -0.5 318,379 - BWI76_RS01560 0.42 -1.7 318,483 - BWI76_RS01560 0.62 -1.0 318,564 + BWI76_RS01560 0.79 -0.2 318,605 - BWI76_RS01560 0.87 +1.0 318,630 + +0.2 318,638 - +0.3 318,668 - -0.4 318,754 + -0.3 319,333 + BWI76_RS01565 0.73 -0.5 319,548 - -0.8
Or see this region's nucleotide sequence