Experiment: B3 MOi 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01315 and BWI76_RS01320 are separated by 76 nucleotides BWI76_RS01320 and BWI76_RS01325 are separated by 3 nucleotides BWI76_RS01325 and BWI76_RS01330 are separated by 2 nucleotides BWI76_RS01330 and BWI76_RS01335 are separated by 42 nucleotides BWI76_RS01335 and BWI76_RS01340 are separated by 6 nucleotides
BWI76_RS01315: BWI76_RS01315 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 269,971 to 271,611
_RS01315
BWI76_RS01320: BWI76_RS01320 - twin-arginine translocase subunit TatA, at 271,688 to 271,939
_RS01320
BWI76_RS01325: BWI76_RS01325 - twin-arginine translocase subunit TatB, at 271,943 to 272,482
_RS01325
BWI76_RS01330: BWI76_RS01330 - twin-arginine translocase subunit TatC, at 272,485 to 273,258
_RS01330
BWI76_RS01335: BWI76_RS01335 - DNase TatD, at 273,301 to 274,083
_RS01335
BWI76_RS01340: BWI76_RS01340 - transcription/translation regulatory transformer protein RfaH, at 274,090 to 274,578
_RS01340
Position (kb) 272
273
274 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 271.595 kb on + strand at 271.692 kb on - strand at 271.933 kb on + strand at 272.135 kb on - strand, within BWI76_RS01325 at 272.158 kb on - strand, within BWI76_RS01325 at 272.347 kb on - strand, within BWI76_RS01325 at 272.522 kb on + strand at 272.781 kb on + strand, within BWI76_RS01330 at 272.796 kb on - strand, within BWI76_RS01330 at 272.822 kb on + strand, within BWI76_RS01330 at 272.835 kb on + strand, within BWI76_RS01330 at 272.915 kb on + strand, within BWI76_RS01330 at 273.194 kb on - strand at 273.210 kb on - strand at 273.253 kb on + strand at 273.358 kb on + strand at 273.407 kb on + strand, within BWI76_RS01335 at 273.479 kb on + strand, within BWI76_RS01335 at 273.615 kb on - strand, within BWI76_RS01335 at 273.799 kb on - strand, within BWI76_RS01335 at 273.848 kb on + strand, within BWI76_RS01335 at 273.985 kb on + strand, within BWI76_RS01335 at 274.028 kb on + strand at 274.028 kb on - strand at 274.036 kb on - strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.123 kb on + strand at 274.131 kb on - strand at 274.131 kb on - strand at 274.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 1 remove 271,595 + -0.2 271,692 - -2.0 271,933 + +0.5 272,135 - BWI76_RS01325 0.36 -3.0 272,158 - BWI76_RS01325 0.40 -1.4 272,347 - BWI76_RS01325 0.75 +2.8 272,522 + +0.6 272,781 + BWI76_RS01330 0.38 -3.5 272,796 - BWI76_RS01330 0.40 -2.7 272,822 + BWI76_RS01330 0.44 -1.4 272,835 + BWI76_RS01330 0.45 +1.0 272,915 + BWI76_RS01330 0.56 -1.4 273,194 - -3.5 273,210 - +0.0 273,253 + +0.1 273,358 + +0.1 273,407 + BWI76_RS01335 0.14 -0.6 273,479 + BWI76_RS01335 0.23 +0.2 273,615 - BWI76_RS01335 0.40 -0.3 273,799 - BWI76_RS01335 0.64 +0.6 273,848 + BWI76_RS01335 0.70 +0.0 273,985 + BWI76_RS01335 0.87 +0.4 274,028 + +0.4 274,028 - +0.4 274,036 - +1.1 274,123 + -0.0 274,123 + -0.3 274,123 + -0.0 274,123 + -0.4 274,131 - +1.9 274,131 - +0.2 274,131 - +0.1
Or see this region's nucleotide sequence