Experiment: B3 MOi 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02815 and BWI76_RS02820 are separated by 175 nucleotides BWI76_RS02820 and BWI76_RS02825 are separated by 64 nucleotides BWI76_RS02825 and BWI76_RS02830 are separated by 162 nucleotides
BWI76_RS02815: BWI76_RS02815 - fructose-bisphosphatase class I, at 574,848 to 575,846
_RS02815
BWI76_RS02820: BWI76_RS02820 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 576,022 to 577,395
_RS02820
BWI76_RS02825: BWI76_RS02825 - putative acyltransferase domain protein, at 577,460 to 578,005
_RS02825
BWI76_RS02830: BWI76_RS02830 - DUF4432 domain-containing protein, at 578,168 to 579,376
_RS02830
Position (kb)
576
577
578 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 575.069 kb on + strand, within BWI76_RS02815 at 575.351 kb on - strand, within BWI76_RS02815 at 575.631 kb on - strand, within BWI76_RS02815 at 575.769 kb on + strand at 575.865 kb on - strand at 575.922 kb on + strand at 575.928 kb on - strand at 575.979 kb on + strand at 576.100 kb on - strand at 576.100 kb on - strand at 576.128 kb on - strand at 576.546 kb on + strand, within BWI76_RS02820 at 576.731 kb on + strand, within BWI76_RS02820 at 576.739 kb on - strand, within BWI76_RS02820 at 576.792 kb on + strand, within BWI76_RS02820 at 576.909 kb on - strand, within BWI76_RS02820 at 577.147 kb on - strand, within BWI76_RS02820 at 577.269 kb on + strand at 577.396 kb on + strand at 577.522 kb on + strand, within BWI76_RS02825 at 577.528 kb on - strand, within BWI76_RS02825 at 577.552 kb on + strand, within BWI76_RS02825 at 577.560 kb on - strand, within BWI76_RS02825 at 577.634 kb on + strand, within BWI76_RS02825 at 577.911 kb on + strand, within BWI76_RS02825 at 578.213 kb on + strand at 578.264 kb on + strand at 578.272 kb on + strand at 578.272 kb on - strand at 578.277 kb on - strand at 578.282 kb on - strand at 578.307 kb on + strand, within BWI76_RS02830 at 578.358 kb on - strand, within BWI76_RS02830
Per-strain Table
Position Strand Gene LocusTag Fraction B3 MOi 10 remove 575,069 + BWI76_RS02815 0.22 +2.1 575,351 - BWI76_RS02815 0.50 +1.3 575,631 - BWI76_RS02815 0.78 +0.5 575,769 + -0.4 575,865 - +0.1 575,922 + +1.0 575,928 - -1.7 575,979 + -0.0 576,100 - -0.4 576,100 - +0.4 576,128 - +1.8 576,546 + BWI76_RS02820 0.38 -0.6 576,731 + BWI76_RS02820 0.52 -0.5 576,739 - BWI76_RS02820 0.52 -1.0 576,792 + BWI76_RS02820 0.56 -0.7 576,909 - BWI76_RS02820 0.65 -0.3 577,147 - BWI76_RS02820 0.82 -0.5 577,269 + -1.1 577,396 + +0.6 577,522 + BWI76_RS02825 0.11 +0.5 577,528 - BWI76_RS02825 0.12 -1.0 577,552 + BWI76_RS02825 0.17 -0.7 577,560 - BWI76_RS02825 0.18 +0.7 577,634 + BWI76_RS02825 0.32 -0.4 577,911 + BWI76_RS02825 0.83 -0.0 578,213 + +0.5 578,264 + +0.6 578,272 + -0.7 578,272 - -0.2 578,277 - -2.2 578,282 - +0.2 578,307 + BWI76_RS02830 0.11 +0.0 578,358 - BWI76_RS02830 0.16 +1.3
Or see this region's nucleotide sequence