Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25190 and BWI76_RS25195 are separated by 198 nucleotides BWI76_RS25195 and BWI76_RS25200 are separated by 56 nucleotides BWI76_RS25200 and BWI76_RS25205 are separated by 54 nucleotides BWI76_RS25205 and BWI76_RS25210 are separated by 135 nucleotides BWI76_RS25210 and BWI76_RS25215 overlap by 1 nucleotides
BWI76_RS25190: BWI76_RS25190 - octaprenyl-diphosphate synthase, at 5,240,907 to 5,241,878
_RS25190
BWI76_RS25195: BWI76_RS25195 - maltose metabolism regulator, at 5,242,077 to 5,242,349
_RS25195
BWI76_RS25200: BWI76_RS25200 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 5,242,406 to 5,243,665
_RS25200
BWI76_RS25205: BWI76_RS25205 - hypothetical protein, at 5,243,720 to 5,243,974
_RS25205
BWI76_RS25210: BWI76_RS25210 - sulfate transporter, at 5,244,110 to 5,244,400
_RS25210
BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035
_RS25215
Position (kb) 5242
5243
5244 Strain fitness (log2 ratio) -1
0
1 at 5241.881 kb on - strand at 5242.065 kb on - strand at 5242.205 kb on + strand, within BWI76_RS25195 at 5242.213 kb on - strand, within BWI76_RS25195 at 5242.354 kb on + strand at 5242.371 kb on + strand at 5243.761 kb on - strand, within BWI76_RS25205 at 5244.001 kb on - strand at 5244.019 kb on - strand at 5244.039 kb on - strand at 5244.176 kb on + strand, within BWI76_RS25210 at 5244.381 kb on + strand at 5244.440 kb on + strand at 5244.624 kb on + strand, within BWI76_RS25215
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 5,241,881 - +1.2 5,242,065 - -0.0 5,242,205 + BWI76_RS25195 0.47 +0.1 5,242,213 - BWI76_RS25195 0.50 +0.6 5,242,354 + +0.2 5,242,371 + -0.7 5,243,761 - BWI76_RS25205 0.16 +1.0 5,244,001 - -0.4 5,244,019 - +0.8 5,244,039 - +0.4 5,244,176 + BWI76_RS25210 0.23 -1.2 5,244,381 + +0.4 5,244,440 + +0.1 5,244,624 + BWI76_RS25215 0.35 +0.0
Or see this region's nucleotide sequence