Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23010 and BWI76_RS23015 overlap by 4 nucleotides BWI76_RS23015 and BWI76_RS23020 are separated by 8 nucleotides BWI76_RS23020 and BWI76_RS23025 overlap by 14 nucleotides BWI76_RS23025 and BWI76_RS23030 are separated by 1 nucleotides
BWI76_RS23010: BWI76_RS23010 - cobyric acid synthase CobQ, at 4,800,515 to 4,802,038
_RS23010
BWI76_RS23015: BWI76_RS23015 - cobalt transporter ATP-binding subunit, at 4,802,035 to 4,802,850
_RS23015
BWI76_RS23020: BWI76_RS23020 - cobalamin biosynthesis protein CbiQ, at 4,802,859 to 4,803,536
_RS23020
BWI76_RS23025: BWI76_RS23025 - cobalt ABC transporter substrate-binding protein CbiN, at 4,803,523 to 4,803,804
_RS23025
BWI76_RS23030: BWI76_RS23030 - cobalamin biosynthesis protein CbiM, at 4,803,806 to 4,804,543
_RS23030
Position (kb)
4802
4803 Strain fitness (log2 ratio)
-1
0
1
2 at 4801.437 kb on - strand, within BWI76_RS23010 at 4801.464 kb on + strand, within BWI76_RS23010 at 4801.464 kb on + strand, within BWI76_RS23010 at 4801.472 kb on - strand, within BWI76_RS23010 at 4801.472 kb on - strand, within BWI76_RS23010 at 4801.472 kb on - strand, within BWI76_RS23010 at 4801.624 kb on + strand, within BWI76_RS23010 at 4801.687 kb on - strand, within BWI76_RS23010 at 4801.687 kb on - strand, within BWI76_RS23010 at 4801.687 kb on - strand, within BWI76_RS23010 at 4801.714 kb on + strand, within BWI76_RS23010 at 4801.839 kb on - strand, within BWI76_RS23010 at 4801.871 kb on + strand, within BWI76_RS23010 at 4802.113 kb on - strand at 4802.288 kb on + strand, within BWI76_RS23015 at 4802.858 kb on - strand at 4802.875 kb on + strand at 4802.875 kb on + strand at 4803.106 kb on + strand, within BWI76_RS23020 at 4803.114 kb on - strand, within BWI76_RS23020 at 4803.392 kb on - strand, within BWI76_RS23020 at 4803.606 kb on - strand, within BWI76_RS23025 at 4803.768 kb on - strand, within BWI76_RS23025
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 4,801,437 - BWI76_RS23010 0.60 -0.0 4,801,464 + BWI76_RS23010 0.62 +0.2 4,801,464 + BWI76_RS23010 0.62 +0.4 4,801,472 - BWI76_RS23010 0.63 -0.4 4,801,472 - BWI76_RS23010 0.63 -0.4 4,801,472 - BWI76_RS23010 0.63 -0.5 4,801,624 + BWI76_RS23010 0.73 +0.6 4,801,687 - BWI76_RS23010 0.77 +0.2 4,801,687 - BWI76_RS23010 0.77 -0.5 4,801,687 - BWI76_RS23010 0.77 +0.4 4,801,714 + BWI76_RS23010 0.79 -0.9 4,801,839 - BWI76_RS23010 0.87 -0.2 4,801,871 + BWI76_RS23010 0.89 +1.1 4,802,113 - +0.7 4,802,288 + BWI76_RS23015 0.31 -0.7 4,802,858 - +1.2 4,802,875 + -0.4 4,802,875 + -0.8 4,803,106 + BWI76_RS23020 0.36 +2.6 4,803,114 - BWI76_RS23020 0.38 +2.0 4,803,392 - BWI76_RS23020 0.79 -0.8 4,803,606 - BWI76_RS23025 0.29 -0.4 4,803,768 - BWI76_RS23025 0.87 -0.3
Or see this region's nucleotide sequence