Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22765 and BWI76_RS22770 are separated by 346 nucleotides BWI76_RS22770 and BWI76_RS22775 overlap by 72 nucleotides BWI76_RS22775 and BWI76_RS22780 are separated by 56 nucleotides BWI76_RS22780 and BWI76_RS22785 are separated by 459 nucleotides
BWI76_RS22765: BWI76_RS22765 - sulfite reductase subunit alpha, at 4,745,693 to 4,747,492
_RS22765
BWI76_RS22770: BWI76_RS22770 - 6-pyruvoyl tetrahydrobiopterin synthase, at 4,747,839 to 4,748,000
_RS22770
BWI76_RS22775: BWI76_RS22775 - 6-carboxytetrahydropterin synthase QueD, at 4,747,929 to 4,748,291
_RS22775
BWI76_RS22780: BWI76_RS22780 - 7-carboxy-7-deazaguanine synthase QueE, at 4,748,348 to 4,749,019
_RS22780
BWI76_RS22785: BWI76_RS22785 - NAD(P)H-binding protein, at 4,749,479 to 4,750,588
_RS22785
Position (kb)
4748
4749
4750 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4747.376 kb on - strand at 4747.376 kb on - strand at 4747.498 kb on + strand at 4747.519 kb on - strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.612 kb on + strand at 4747.613 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.620 kb on - strand at 4747.677 kb on + strand at 4747.692 kb on - strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.943 kb on + strand at 4747.957 kb on + strand at 4747.968 kb on + strand at 4748.026 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.096 kb on + strand, within BWI76_RS22775 at 4748.165 kb on + strand, within BWI76_RS22775 at 4748.187 kb on - strand, within BWI76_RS22775 at 4748.330 kb on - strand at 4748.401 kb on - strand at 4748.468 kb on - strand, within BWI76_RS22780 at 4748.605 kb on + strand, within BWI76_RS22780 at 4748.613 kb on - strand, within BWI76_RS22780 at 4748.627 kb on + strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.635 kb on - strand, within BWI76_RS22780 at 4748.774 kb on - strand, within BWI76_RS22780 at 4748.779 kb on - strand, within BWI76_RS22780 at 4748.833 kb on + strand, within BWI76_RS22780 at 4748.884 kb on + strand, within BWI76_RS22780 at 4748.972 kb on + strand at 4748.974 kb on - strand at 4748.987 kb on + strand at 4748.988 kb on + strand at 4748.996 kb on - strand at 4749.014 kb on - strand at 4749.030 kb on + strand at 4749.068 kb on + strand at 4749.073 kb on + strand at 4749.073 kb on + strand at 4749.079 kb on + strand at 4749.081 kb on - strand at 4749.082 kb on - strand at 4749.084 kb on + strand at 4749.118 kb on + strand at 4749.126 kb on - strand at 4749.128 kb on + strand at 4749.134 kb on - strand at 4749.134 kb on - strand at 4749.156 kb on - strand at 4749.196 kb on + strand at 4749.204 kb on - strand at 4749.204 kb on - strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.238 kb on + strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.246 kb on - strand at 4749.305 kb on - strand at 4749.305 kb on - strand at 4749.330 kb on + strand at 4749.350 kb on - strand at 4749.389 kb on - strand at 4749.459 kb on + strand at 4749.467 kb on - strand at 4749.473 kb on + strand at 4749.517 kb on + strand at 4749.517 kb on + strand at 4749.517 kb on + strand at 4749.522 kb on + strand at 4749.522 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.527 kb on + strand at 4749.535 kb on - strand at 4749.535 kb on - strand at 4749.572 kb on + strand at 4749.572 kb on - strand at 4749.572 kb on - strand at 4749.574 kb on - strand at 4749.580 kb on - strand at 4749.580 kb on - strand at 4749.587 kb on + strand at 4749.594 kb on + strand, within BWI76_RS22785 at 4749.608 kb on + strand, within BWI76_RS22785 at 4749.612 kb on + strand, within BWI76_RS22785 at 4749.613 kb on + strand, within BWI76_RS22785 at 4749.613 kb on + strand, within BWI76_RS22785 at 4749.619 kb on + strand, within BWI76_RS22785 at 4749.619 kb on + strand, within BWI76_RS22785 at 4749.626 kb on - strand, within BWI76_RS22785 at 4749.626 kb on - strand, within BWI76_RS22785 at 4749.662 kb on + strand, within BWI76_RS22785 at 4749.662 kb on + strand, within BWI76_RS22785 at 4749.664 kb on + strand, within BWI76_RS22785 at 4749.675 kb on + strand, within BWI76_RS22785 at 4749.679 kb on - strand, within BWI76_RS22785 at 4749.681 kb on - strand, within BWI76_RS22785 at 4749.686 kb on - strand, within BWI76_RS22785 at 4749.691 kb on - strand, within BWI76_RS22785 at 4749.752 kb on - strand, within BWI76_RS22785 at 4749.757 kb on - strand, within BWI76_RS22785 at 4749.847 kb on + strand, within BWI76_RS22785 at 4749.897 kb on - strand, within BWI76_RS22785 at 4749.897 kb on - strand, within BWI76_RS22785
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 4,747,376 - +0.1 4,747,376 - +0.5 4,747,498 + -1.4 4,747,519 - -0.3 4,747,612 + +1.6 4,747,612 + -0.4 4,747,612 + -0.1 4,747,613 - -0.7 4,747,620 - +0.6 4,747,620 - +1.6 4,747,620 - +0.0 4,747,620 - -0.8 4,747,677 + -0.2 4,747,692 - +0.0 4,747,943 + -0.2 4,747,943 + -0.6 4,747,943 + +0.1 4,747,957 + +0.3 4,747,968 + +0.1 4,748,026 + BWI76_RS22775 0.27 +0.3 4,748,096 + BWI76_RS22775 0.46 -0.0 4,748,096 + BWI76_RS22775 0.46 +0.0 4,748,165 + BWI76_RS22775 0.65 +0.0 4,748,187 - BWI76_RS22775 0.71 +0.9 4,748,330 - -0.1 4,748,401 - -1.4 4,748,468 - BWI76_RS22780 0.18 +0.5 4,748,605 + BWI76_RS22780 0.38 -0.1 4,748,613 - BWI76_RS22780 0.39 +0.0 4,748,627 + BWI76_RS22780 0.42 -0.6 4,748,635 - BWI76_RS22780 0.43 -0.5 4,748,635 - BWI76_RS22780 0.43 +1.1 4,748,774 - BWI76_RS22780 0.63 +1.4 4,748,779 - BWI76_RS22780 0.64 +0.4 4,748,833 + BWI76_RS22780 0.72 +1.5 4,748,884 + BWI76_RS22780 0.80 +0.7 4,748,972 + +0.8 4,748,974 - -0.8 4,748,987 + +0.1 4,748,988 + +0.8 4,748,996 - +0.8 4,749,014 - +0.1 4,749,030 + +1.0 4,749,068 + -0.0 4,749,073 + -0.6 4,749,073 + +0.7 4,749,079 + -2.4 4,749,081 - +0.0 4,749,082 - -0.3 4,749,084 + -0.3 4,749,118 + +0.4 4,749,126 - -1.1 4,749,128 + +0.4 4,749,134 - -0.4 4,749,134 - +1.4 4,749,156 - +1.0 4,749,196 + +0.1 4,749,204 - -0.3 4,749,204 - +0.1 4,749,238 + +0.1 4,749,238 + -0.0 4,749,238 + -0.8 4,749,246 - +0.2 4,749,246 - +0.5 4,749,246 - +0.2 4,749,246 - -1.2 4,749,305 - +1.8 4,749,305 - -1.2 4,749,330 + +0.4 4,749,350 - +0.4 4,749,389 - -0.2 4,749,459 + -0.3 4,749,467 - -0.1 4,749,473 + -1.3 4,749,517 + +1.7 4,749,517 + +1.4 4,749,517 + -0.4 4,749,522 + -2.4 4,749,522 + -0.9 4,749,527 + +0.6 4,749,527 + -0.9 4,749,527 + -0.1 4,749,527 + -1.0 4,749,527 + +0.4 4,749,527 + -0.2 4,749,527 + +1.1 4,749,535 - -0.9 4,749,535 - +0.8 4,749,572 + -0.2 4,749,572 - +0.6 4,749,572 - +1.4 4,749,574 - -0.3 4,749,580 - +0.2 4,749,580 - +0.3 4,749,587 + +0.3 4,749,594 + BWI76_RS22785 0.10 +0.0 4,749,608 + BWI76_RS22785 0.12 +1.2 4,749,612 + BWI76_RS22785 0.12 -2.4 4,749,613 + BWI76_RS22785 0.12 +0.6 4,749,613 + BWI76_RS22785 0.12 +0.3 4,749,619 + BWI76_RS22785 0.13 -0.7 4,749,619 + BWI76_RS22785 0.13 +0.5 4,749,626 - BWI76_RS22785 0.13 -1.4 4,749,626 - BWI76_RS22785 0.13 -0.4 4,749,662 + BWI76_RS22785 0.16 -0.8 4,749,662 + BWI76_RS22785 0.16 -0.0 4,749,664 + BWI76_RS22785 0.17 +0.9 4,749,675 + BWI76_RS22785 0.18 +1.1 4,749,679 - BWI76_RS22785 0.18 -1.4 4,749,681 - BWI76_RS22785 0.18 +0.2 4,749,686 - BWI76_RS22785 0.19 -0.8 4,749,691 - BWI76_RS22785 0.19 +1.8 4,749,752 - BWI76_RS22785 0.25 -0.1 4,749,757 - BWI76_RS22785 0.25 +0.6 4,749,847 + BWI76_RS22785 0.33 -0.3 4,749,897 - BWI76_RS22785 0.38 +0.2 4,749,897 - BWI76_RS22785 0.38 -0.1
Or see this region's nucleotide sequence