Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19550

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19540 and BWI76_RS19545 are separated by 119 nucleotidesBWI76_RS19545 and BWI76_RS19550 are separated by 96 nucleotidesBWI76_RS19550 and BWI76_RS19555 are separated by 17 nucleotides BWI76_RS19540: BWI76_RS19540 - multidrug transporter permease, at 4,083,838 to 4,085,280 _RS19540 BWI76_RS19545: BWI76_RS19545 - type 11 methyltransferase, at 4,085,400 to 4,086,134 _RS19545 BWI76_RS19550: BWI76_RS19550 - phospho-cellobiase, at 4,086,231 to 4,087,625 _RS19550 BWI76_RS19555: BWI76_RS19555 - Beta-glucoside phosphotransferase system, IIA/IIB/IIC components (from data), at 4,087,643 to 4,089,502 _RS19555 Position (kb) 4086 4087 4088Strain fitness (log2 ratio) -1 0 1at 4086.341 kb on + strandat 4086.397 kb on - strand, within BWI76_RS19550at 4086.517 kb on + strand, within BWI76_RS19550at 4086.517 kb on + strand, within BWI76_RS19550at 4087.281 kb on - strand, within BWI76_RS19550at 4087.486 kb on + strandat 4087.523 kb on + strandat 4087.533 kb on + strandat 4087.574 kb on + strandat 4088.067 kb on - strand, within BWI76_RS19555at 4088.172 kb on - strand, within BWI76_RS19555at 4088.207 kb on + strand, within BWI76_RS19555at 4088.365 kb on - strand, within BWI76_RS19555

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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4,086,341 + -0.7
4,086,397 - BWI76_RS19550 0.12 -0.6
4,086,517 + BWI76_RS19550 0.21 -1.0
4,086,517 + BWI76_RS19550 0.21 -0.7
4,087,281 - BWI76_RS19550 0.75 -0.8
4,087,486 + -0.9
4,087,523 + +0.0
4,087,533 + +0.0
4,087,574 + +0.1
4,088,067 - BWI76_RS19555 0.23 +0.4
4,088,172 - BWI76_RS19555 0.28 +0.0
4,088,207 + BWI76_RS19555 0.30 -0.2
4,088,365 - BWI76_RS19555 0.39 -0.7

Or see this region's nucleotide sequence