Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19535

Experiment: KLEB24 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19525 and BWI76_RS19530 are separated by 174 nucleotidesBWI76_RS19530 and BWI76_RS19535 are separated by 155 nucleotidesBWI76_RS19535 and BWI76_RS19540 are separated by 105 nucleotides BWI76_RS19525: BWI76_RS19525 - D-alanyl-D-alanine endopeptidase, at 4,081,298 to 4,082,245 _RS19525 BWI76_RS19530: BWI76_RS19530 - YIP1 family protein, at 4,082,420 to 4,083,007 _RS19530 BWI76_RS19535: BWI76_RS19535 - putative integral membrane protein, at 4,083,163 to 4,083,732 _RS19535 BWI76_RS19540: BWI76_RS19540 - multidrug transporter permease, at 4,083,838 to 4,085,280 _RS19540 Position (kb) 4083 4084Strain fitness (log2 ratio) -2 -1 0 1at 4082.209 kb on + strandat 4082.217 kb on - strandat 4082.217 kb on - strandat 4082.307 kb on - strandat 4082.309 kb on - strandat 4082.309 kb on - strandat 4082.426 kb on + strandat 4082.455 kb on - strandat 4082.510 kb on + strand, within BWI76_RS19530at 4082.580 kb on + strand, within BWI76_RS19530at 4082.580 kb on + strand, within BWI76_RS19530at 4082.801 kb on - strand, within BWI76_RS19530at 4082.925 kb on - strand, within BWI76_RS19530at 4083.031 kb on + strandat 4083.039 kb on - strandat 4083.120 kb on + strandat 4083.254 kb on - strand, within BWI76_RS19535at 4083.273 kb on - strand, within BWI76_RS19535at 4083.432 kb on - strand, within BWI76_RS19535at 4083.767 kb on - strandat 4083.860 kb on + strandat 4084.216 kb on + strand, within BWI76_RS19540at 4084.613 kb on + strand, within BWI76_RS19540at 4084.636 kb on - strand, within BWI76_RS19540

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB24 MOI 1
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4,082,209 + -2.1
4,082,217 - +0.1
4,082,217 - -1.4
4,082,307 - -0.3
4,082,309 - -0.5
4,082,309 - -0.4
4,082,426 + +0.2
4,082,455 - +1.6
4,082,510 + BWI76_RS19530 0.15 -0.4
4,082,580 + BWI76_RS19530 0.27 -0.8
4,082,580 + BWI76_RS19530 0.27 -0.8
4,082,801 - BWI76_RS19530 0.65 -0.2
4,082,925 - BWI76_RS19530 0.86 +0.9
4,083,031 + -0.4
4,083,039 - -0.7
4,083,120 + -0.4
4,083,254 - BWI76_RS19535 0.16 -2.0
4,083,273 - BWI76_RS19535 0.19 -0.8
4,083,432 - BWI76_RS19535 0.47 -1.4
4,083,767 - -1.6
4,083,860 + +0.1
4,084,216 + BWI76_RS19540 0.26 -0.6
4,084,613 + BWI76_RS19540 0.54 +0.5
4,084,636 - BWI76_RS19540 0.55 -0.3

Or see this region's nucleotide sequence