Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18345 and BWI76_RS18350 are separated by 288 nucleotides BWI76_RS18350 and BWI76_RS18355 overlap by 4 nucleotides BWI76_RS18355 and BWI76_RS18360 are separated by 15 nucleotides
BWI76_RS18345: BWI76_RS18345 - transcriptional regulator SdiA, at 3,808,816 to 3,809,538
_RS18345
BWI76_RS18350: BWI76_RS18350 - L-cystine ABC transporter ATP-binding protein YecC, at 3,809,827 to 3,810,579
_RS18350
BWI76_RS18355: BWI76_RS18355 - amino acid ABC transporter permease, at 3,810,576 to 3,811,244
_RS18355
BWI76_RS18360: BWI76_RS18360 - aminocyclopropane-1-carboxylate deaminase/D-cysteine desulfhydrase family protein, at 3,811,260 to 3,812,246
_RS18360
Position (kb)
3809
3810
3811 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3808.904 kb on - strand, within BWI76_RS18345 at 3808.957 kb on + strand, within BWI76_RS18345 at 3808.960 kb on - strand, within BWI76_RS18345 at 3809.027 kb on - strand, within BWI76_RS18345 at 3809.045 kb on + strand, within BWI76_RS18345 at 3809.046 kb on + strand, within BWI76_RS18345 at 3809.063 kb on - strand, within BWI76_RS18345 at 3809.063 kb on - strand, within BWI76_RS18345 at 3809.100 kb on + strand, within BWI76_RS18345 at 3809.109 kb on + strand, within BWI76_RS18345 at 3809.110 kb on + strand, within BWI76_RS18345 at 3809.115 kb on + strand, within BWI76_RS18345 at 3809.156 kb on + strand, within BWI76_RS18345 at 3809.164 kb on - strand, within BWI76_RS18345 at 3809.164 kb on - strand, within BWI76_RS18345 at 3809.164 kb on - strand, within BWI76_RS18345 at 3809.164 kb on - strand, within BWI76_RS18345 at 3809.166 kb on + strand, within BWI76_RS18345 at 3809.174 kb on - strand, within BWI76_RS18345 at 3809.174 kb on - strand, within BWI76_RS18345 at 3809.174 kb on - strand, within BWI76_RS18345 at 3809.174 kb on - strand, within BWI76_RS18345 at 3809.174 kb on - strand, within BWI76_RS18345 at 3809.176 kb on - strand, within BWI76_RS18345 at 3809.178 kb on + strand, within BWI76_RS18345 at 3809.292 kb on + strand, within BWI76_RS18345 at 3809.292 kb on + strand, within BWI76_RS18345 at 3809.295 kb on - strand, within BWI76_RS18345 at 3809.300 kb on - strand, within BWI76_RS18345 at 3809.300 kb on - strand, within BWI76_RS18345 at 3809.300 kb on - strand, within BWI76_RS18345 at 3809.336 kb on + strand, within BWI76_RS18345 at 3809.336 kb on + strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.344 kb on - strand, within BWI76_RS18345 at 3809.349 kb on - strand, within BWI76_RS18345 at 3809.354 kb on + strand, within BWI76_RS18345 at 3809.372 kb on - strand, within BWI76_RS18345 at 3809.372 kb on - strand, within BWI76_RS18345 at 3809.377 kb on - strand, within BWI76_RS18345 at 3809.377 kb on - strand, within BWI76_RS18345 at 3809.387 kb on + strand, within BWI76_RS18345 at 3809.397 kb on + strand, within BWI76_RS18345 at 3809.405 kb on - strand, within BWI76_RS18345 at 3809.405 kb on - strand, within BWI76_RS18345 at 3809.405 kb on - strand, within BWI76_RS18345 at 3809.417 kb on - strand, within BWI76_RS18345 at 3809.469 kb on + strand at 3809.477 kb on - strand at 3809.541 kb on - strand at 3809.548 kb on + strand at 3809.553 kb on - strand at 3809.556 kb on - strand at 3809.556 kb on - strand at 3809.556 kb on - strand at 3809.695 kb on - strand at 3809.698 kb on + strand at 3809.698 kb on + strand at 3809.698 kb on + strand at 3809.706 kb on - strand at 3809.723 kb on + strand at 3809.723 kb on + strand at 3809.723 kb on + strand at 3809.723 kb on + strand at 3809.723 kb on - strand at 3809.731 kb on - strand at 3809.748 kb on - strand at 3809.791 kb on + strand at 3809.799 kb on - strand at 3809.806 kb on + strand at 3809.819 kb on - strand at 3809.861 kb on + strand at 3809.866 kb on + strand at 3810.023 kb on + strand, within BWI76_RS18350 at 3810.198 kb on + strand, within BWI76_RS18350 at 3810.276 kb on + strand, within BWI76_RS18350 at 3810.324 kb on - strand, within BWI76_RS18350 at 3810.357 kb on + strand, within BWI76_RS18350 at 3810.365 kb on - strand, within BWI76_RS18350 at 3810.437 kb on - strand, within BWI76_RS18350 at 3810.518 kb on - strand at 3810.600 kb on + strand at 3810.724 kb on + strand, within BWI76_RS18355 at 3810.737 kb on - strand, within BWI76_RS18355 at 3811.005 kb on - strand, within BWI76_RS18355 at 3811.173 kb on - strand, within BWI76_RS18355 at 3811.226 kb on + strand at 3811.234 kb on - strand at 3811.286 kb on + strand at 3811.486 kb on + strand, within BWI76_RS18360
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 3,808,904 - BWI76_RS18345 0.12 +0.3 3,808,957 + BWI76_RS18345 0.20 -1.5 3,808,960 - BWI76_RS18345 0.20 +0.8 3,809,027 - BWI76_RS18345 0.29 -2.1 3,809,045 + BWI76_RS18345 0.32 -1.5 3,809,046 + BWI76_RS18345 0.32 +1.5 3,809,063 - BWI76_RS18345 0.34 +0.0 3,809,063 - BWI76_RS18345 0.34 -1.3 3,809,100 + BWI76_RS18345 0.39 -0.1 3,809,109 + BWI76_RS18345 0.41 -2.3 3,809,110 + BWI76_RS18345 0.41 +0.7 3,809,115 + BWI76_RS18345 0.41 -0.5 3,809,156 + BWI76_RS18345 0.47 +0.4 3,809,164 - BWI76_RS18345 0.48 +0.3 3,809,164 - BWI76_RS18345 0.48 -0.2 3,809,164 - BWI76_RS18345 0.48 +0.5 3,809,164 - BWI76_RS18345 0.48 +2.1 3,809,166 + BWI76_RS18345 0.48 +2.1 3,809,174 - BWI76_RS18345 0.50 -0.0 3,809,174 - BWI76_RS18345 0.50 +2.2 3,809,174 - BWI76_RS18345 0.50 -0.2 3,809,174 - BWI76_RS18345 0.50 +0.6 3,809,174 - BWI76_RS18345 0.50 +1.1 3,809,176 - BWI76_RS18345 0.50 -0.4 3,809,178 + BWI76_RS18345 0.50 -1.0 3,809,292 + BWI76_RS18345 0.66 -1.0 3,809,292 + BWI76_RS18345 0.66 -0.2 3,809,295 - BWI76_RS18345 0.66 +1.7 3,809,300 - BWI76_RS18345 0.67 -0.1 3,809,300 - BWI76_RS18345 0.67 -0.5 3,809,300 - BWI76_RS18345 0.67 -0.9 3,809,336 + BWI76_RS18345 0.72 -0.5 3,809,336 + BWI76_RS18345 0.72 +0.8 3,809,344 - BWI76_RS18345 0.73 +0.1 3,809,344 - BWI76_RS18345 0.73 +0.8 3,809,344 - BWI76_RS18345 0.73 -0.1 3,809,344 - BWI76_RS18345 0.73 +0.3 3,809,344 - BWI76_RS18345 0.73 +0.3 3,809,344 - BWI76_RS18345 0.73 -0.1 3,809,344 - BWI76_RS18345 0.73 -1.1 3,809,349 - BWI76_RS18345 0.74 +2.1 3,809,354 + BWI76_RS18345 0.74 +0.7 3,809,372 - BWI76_RS18345 0.77 +0.6 3,809,372 - BWI76_RS18345 0.77 +1.1 3,809,377 - BWI76_RS18345 0.78 +0.8 3,809,377 - BWI76_RS18345 0.78 +0.6 3,809,387 + BWI76_RS18345 0.79 -0.9 3,809,397 + BWI76_RS18345 0.80 -0.2 3,809,405 - BWI76_RS18345 0.81 -0.4 3,809,405 - BWI76_RS18345 0.81 -0.7 3,809,405 - BWI76_RS18345 0.81 -0.1 3,809,417 - BWI76_RS18345 0.83 +0.5 3,809,469 + -0.2 3,809,477 - +0.0 3,809,541 - -0.5 3,809,548 + +0.5 3,809,553 - -0.4 3,809,556 - +0.7 3,809,556 - -0.3 3,809,556 - +0.3 3,809,695 - -0.5 3,809,698 + +0.4 3,809,698 + +0.3 3,809,698 + -0.2 3,809,706 - -0.4 3,809,723 + +0.5 3,809,723 + +0.9 3,809,723 + -0.1 3,809,723 + -1.6 3,809,723 - -0.8 3,809,731 - -0.4 3,809,748 - -0.2 3,809,791 + -0.2 3,809,799 - +1.5 3,809,806 + -2.3 3,809,819 - -0.6 3,809,861 + +2.7 3,809,866 + +0.8 3,810,023 + BWI76_RS18350 0.26 -0.9 3,810,198 + BWI76_RS18350 0.49 -0.6 3,810,276 + BWI76_RS18350 0.60 -1.1 3,810,324 - BWI76_RS18350 0.66 -0.5 3,810,357 + BWI76_RS18350 0.70 -0.9 3,810,365 - BWI76_RS18350 0.71 -1.7 3,810,437 - BWI76_RS18350 0.81 -1.0 3,810,518 - +0.4 3,810,600 + -0.4 3,810,724 + BWI76_RS18355 0.22 -0.8 3,810,737 - BWI76_RS18355 0.24 -0.3 3,811,005 - BWI76_RS18355 0.64 +0.9 3,811,173 - BWI76_RS18355 0.89 -0.2 3,811,226 + -2.1 3,811,234 - -0.3 3,811,286 + -0.6 3,811,486 + BWI76_RS18360 0.23 +0.1
Or see this region's nucleotide sequence