Experiment: KLEB24 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18130 and BWI76_RS18135 are separated by 78 nucleotides BWI76_RS18135 and BWI76_RS18140 overlap by 1 nucleotides BWI76_RS18140 and BWI76_RS18145 are separated by 198 nucleotides
BWI76_RS18130: BWI76_RS18130 - zinc ABC transporter substrate-binding protein, at 3,768,651 to 3,769,595
_RS18130
BWI76_RS18135: BWI76_RS18135 - zinc ABC transporter ATP-binding protein ZnuC, at 3,769,674 to 3,770,426
_RS18135
BWI76_RS18140: BWI76_RS18140 - zinc ABC transporter permease, at 3,770,426 to 3,771,211
_RS18140
BWI76_RS18145: BWI76_RS18145 - Holliday junction branch migration DNA helicase RuvB, at 3,771,410 to 3,772,420
_RS18145
Position (kb)
3770
3771
3772 Strain fitness (log2 ratio)
-2
-1
0
1 at 3769.758 kb on - strand, within BWI76_RS18135 at 3769.758 kb on - strand, within BWI76_RS18135 at 3769.778 kb on + strand, within BWI76_RS18135 at 3769.890 kb on - strand, within BWI76_RS18135 at 3769.991 kb on - strand, within BWI76_RS18135 at 3770.110 kb on + strand, within BWI76_RS18135 at 3770.122 kb on + strand, within BWI76_RS18135 at 3770.542 kb on - strand, within BWI76_RS18140 at 3770.578 kb on + strand, within BWI76_RS18140 at 3770.611 kb on - strand, within BWI76_RS18140 at 3771.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB24 MOI 1 remove 3,769,758 - BWI76_RS18135 0.11 -1.5 3,769,758 - BWI76_RS18135 0.11 -1.0 3,769,778 + BWI76_RS18135 0.14 +1.1 3,769,890 - BWI76_RS18135 0.29 -0.4 3,769,991 - BWI76_RS18135 0.42 -0.1 3,770,110 + BWI76_RS18135 0.58 -0.3 3,770,122 + BWI76_RS18135 0.59 +0.2 3,770,542 - BWI76_RS18140 0.15 -1.7 3,770,578 + BWI76_RS18140 0.19 -0.5 3,770,611 - BWI76_RS18140 0.24 -2.6 3,771,435 - +0.9
Or see this region's nucleotide sequence